ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Separation and identification of lipase - producing strains and immobilized transesterification efficiency
Jing Li1, 2, Shu Zhang1, Guanghua Liu1, Rui Zhao1, Hui Xu, Dairong Qiao1 and Yi Cao1*
1Microbial and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, 610064, China.
2College of Material and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China.
Accepted 26 January, 2014
Abstract
12 strains of lipase - producing were isolated from 36 samples collected from oil - contaminated soil of machinery - repair plant, dining room, and vegetable market, respectively, of which strain (3 - 2) had the highest hydrolytic activity (17.1 U/ml). Immobilized cell of strain 3 - 2 onto sodium alginate and immobilized crude enzyme solution of strain (3 - 2) onto Sodium alginate were respectively used in two transesterification programs to transfer the Fatty acid methyl ester (FAME). Results showed that both immobilized crude enzyme solution and cell were of high transesterification efficiency for Transesterification Program I, respectively 32.72 and 26.84%. Cycle test done in this Program found that immobilized crude enzyme solution and cell still keep some transesterification efficiency after being recycled thrice at least. Meanwhile, hydrolytic activity of crude enzyme solution had some correlation with transesterification efficiency. However, hydrolytic activity of immobilized cell did not have a clear correlation with the latter. Strain (3 - 2) belonged to Sphingobacterium multivorum. Up to now, the transesterification research has not been reported.
Key words: Lipase, crude enzyme solution, bacterial cell, immobilized, transesterification.
Guanghua Liu, Hui Xu, Jing Li, Shu Zhang, Rui Zhao, Dairong Qiao and Yi Cao*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Improved purification and some properties of a novel phospholipase C from Bacillus mycoides strain 970
Chang Gao Wang1, 2*, Ming Kai Chen3 and Tao Chen1
1Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
2Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei University of Technology, Wuhan 430068, China.
3Renmin Hospital of Wuhan University, Wuhan 430060, China.
Accepted 15 December, 2014
Abstract
A novel microbial phospholipase C from Bacillus mycoides strain 970 was purified to apparent homogeneity by an improved purification process, in which DEAE-Cellulose adsorption was first used to remove bulk of dark brown impurities and then a combination of DEAE-Cellulose ion-exchange, Phenyl Sepharose hydrophobic interaction and Sephacryl S-100 gel filtration chromatography was used to separate the enzyme from remained contaminants. The enzyme appeared to be a single peptide of 75.1 kDa on SDS-PAGE. The optimum temperature and pH of the enzyme were 60°C and 7.0 - 7.5, respectively. The enzyme was stable at temperatures lower than 50°C and pH 5 - 9.5. This purified phospholipase C was characterized as a metallophospholipase C and Ca2+, Mg2+ , Zn2+, Ba2+ were essential for its PLC activity. The enzyme showed no lecithinase activity on egg yolk agar and also no hemolytic activity on blood agar.
Key words: Phospholipase C, purification, metalloenzyme, nonhemolytic activity, Bacillus mycoides.
Ming Kai Chen and Tao Chen, Chang Gao Wang*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Antiviral effect of Hibiscus sabdariffa and Celosia argentea on measles virus
Omilabu A. Sunday1*, A. Bankole Munir5, O. Oyefolu Akeeb3, A. Adesanya Bolanle2 and S. O. Badaru4
1Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Akoka, Lagos, Nigeria.
2Department of Botany and Microbiology, University of Lagos, Akoka, Lagos, Nigeria.
3Department of Microbiology, Lagos State University, Ojo, Lagos, Nigeria.
4Central Public Laboratory, Ministry of Health, Yaba, Lagos, Nigeria.
5Ministry of Health Lagos State, Ikeja, Lagos, Nigeria.
Accepted 17 December, 2014
Abstract
Studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last two decades. The leaves of Hibiscus sabdariffa (red and green leaved) and Celosia argentea were studied for their antiviral activities against Measles Virus (MV) as well as the effects of the extracts on Hep -2 cells. Ethanol extract of the leaves of each of these plants showed no toxicity to the Hep-2 cells at all concentrations used (5, 10 and 15 mg/ml). The pre-inoculative treatment of Hep-2 cells with plant extracts showed that C. argentea had no antiviral activities on MV at all concentrations (5, 10, 15 mg/ml) while H. sabdariffa had antiviral activities only at 10 and 15 mg/ml on MV. The post-inoculative treatment of Hep-2 cells with plant extracts showed that at 5, 10 and 15 mg/ml concentrations, H. sabdariffa had antiviral activities on MV while the antiviral activity of C. argentea could not be established at 10 and 15 mg/ml but did not show any antiviral activity at 5 mg/ml.
Key words: Hibiscus sabdariffa, Celosia argentea, antiviral, toxic, Hep-2 cell, herb, medicinal plant.
A. Adesanya Bolanle and S. O. Badaru, A. Bankole Munir, O. Oyefolu Akeeb, Omilabu A. Sunday*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
In vitro antimicrobial activity of Muntingia calabura extracts and fractions
Z. A. Zakaria1*, A. S. Sufian2, K. Ramasamy2, N. Ahmat3, M. R. Sulaiman1, A. K. Arifah4, A. Zuraini1 and M. N. Somchit1
1Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
2Faculty of Pharmacy, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
3Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
4Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Accepted 5 December, 2014
Abstract
The aim of the present study is to determine the in vitro antimicrobial activity of various extracts, partitions and fractions of Muntingia calabura (Elaeocarpaceae) leaves against a selected panel of microorganisms. The leaves of M. calabura were soaked separately in the aqueous, chloroform and methanol solvent systems in the ratio of 1:20 (w/v) for 72 h and these procedures were repeated three times. Antimicrobial testing was carried out using the micro -broth dilution method. The microbes targeted were Staphylococcus aureus 25923, S. aureus 33591 (a multi-drugs resistant S. aureus (MRSA) isolate), Escherichia coli 35218, Pseudomonas aeruginosa ATCC 27853, Candida albicans 10231 and Microsporum canis ATCC 36299. The methanol extract inhibited MSSA (MIC = 1250 µg/ml; MBC = 1250 µg/ml) and MRSA (MIC = 1250 µg/ml; MBC = 1250 µg /ml) and considered the most effective extract and was further partitioned sequentially using the aqueous, petroleum ether and ethyl acetate. The ethyl acetate partition exhibited effective antibacterial activities with the MIC/MBC value of 156 and 313 µg/ml against S. aureus 25923 and S. aureus 33591, respectively. The ethyl acetate partition underwent fractionation process and yielded 15 fractions (A1-A15) of which only fractions A9 to A15 effectively inhibited the growth of S. aureus 25923 and S. aureus 33591 with MIC/MBC values ranging from 78 to 2500 µg/ml.
Key words: Muntingia calabura, Elaeocarpaceae, antimicrobial activity, micro-broth dilution, ethyl acetate fraction.
K. Ramasamy, A. S. Sufian, Z. A. Zakaria*, A. K. Arifah, N. Ahmat, A. Zuraini and M. N. Somchit, M. R. Sulaiman
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Screening and optimization of nutrients for L-asparaginase production by Bacillus cereus MNTG-7 in SmF by plackett- burmann design
M. Sunitha*, P. Ellaiah and R. Bhavani Devi
Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003, India.
Accepted 17 December, 2014
Abstract
The medium components influencing the enzyme production were screened using Plackett-Burman design. A total of 67 nutrients comprising of fifteen each of carbon, nitrogen, mineral/salt and eleven each of inorganic nitrogen sources and amino acid sources were screened for the production of L-asparaginase by SmF. This design involves screening of up to ‘n-1’ variables in just ‘n’ number of experiments. Regression coefficients and t-values were calculated by subjecting the experimental data to statistical analysis. Among the 67 nutrients based on their performance in terms of product yield and cost, tapioca starch, L-asparagine, ammonium oxalate, gelatin and CaCO3 were identified as most effective and therefore selected for inclusion in surface methodology studies.
Key words: L-asparaginase, nutritional factors, submerged fermentation, Bacillus cereus MNTG-7.
M. Sunitha*, P. Ellaiah and R. Bhavani Devi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Chemical composition of lavender essential oil and its antioxidant activity and inhibition against rhinitis-related bacteria
Lu Hui1, 2, Li He 2, Lu Huan1, Li XiaoLan3* and Zhou AiGuo4
1Teaching and Research Section of Anthropotomy, Medical School of ShiHeZi University, ShiHeZi city, Xinjiang 832008, PR China.
2Department of Anthropotomy and histoembryology, TongJi Medical College of HuaZhong University of Science and Technology, Wuhan, PR China.
3Ear-Nose-Throat (ENT) Department of the First Affiliated Hospital of ShiHeZi University, ShiHeZi city, XinJiang 832008, PR China.
4Acute Surgery Department of the First Affiliated Hospital of ShiHeZi University, ShiHeZi city, PR China.
Accepted 22nd January, 2014
Abstract
In this study we assessed the chemical composition, antioxidant and antibacterial activities of Lavender essential oil. The antioxidant and antibacterial capacity of test sample was assayed by a linoleic acid system and conventional method of bacterial growth inhibition. The results demonstrated that the essential oil consisted of 1,5-Dimethyl-1-vinyl-4-hexenyl but yrate as the most abundant component (43.73%), followed by 1,3,7-Octatriene, 3,7-dimethyl- (25.10%), Eucalyptol (7.32%), and Camphor (3.79%). Lavender essential oil display the stronger antioxidant activity against lipid peroxidation in a linoleic acid model system and good antibacterial activity against four rhinitis-related bacteria including staphylococcus aureus, Micrococcus ascoformans, Proteus vulgaris and Escherichia coli.
Key words: Antibacterial, Lavender; antioxidant, essential oil, extraction, rhinitis.
Lu Huan, Li XiaoLan* and Zhou AiGuo, Lu Hui, Li He
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