ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-008, June, 2019. © International Scholars Journals
Full Length Research Paper
Identify potential chronic heart failure related transcription factors by network analysis
Wu Gang1*, Xie Qiang2#, Cheng Mian3, Jiang Xuejun1, Yang Bo1 and Huang He1
1Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China.
2Department of Cardiovascular disease, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China.
3Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Accepted 23 April, 2019
Abstract
Chronic heart failure (CHF) is characterized by diminished cardiac output and pooling of blood in the venous system. Using transcriptome data, we constructed a regulation network to identify the potential genes that related to heart failure. In the network, some of transcription factors and its’ target genes have been proved to be related to heart failure in previous study. According to the regulation network, we found some new transcription factors and target genes, which have not been proved to be directly related with; we also found MYC, TP53 and ETS2 regulate each other. Our work demonstrated that regulation of network analysis is useful in identification of the candidate genes in heart failure.
Key words: Chronic heart failure, transcription factors, regulation network.
Cheng Mian, Xie Qiang#, Jiang Xuejun, Wu Gang*, Yang Bo and Huang He
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-004, June, 2019. © International Scholars Journals
Full Length Research Paper
The effect of Ivermectin pour-on administration against natural Nematodirus spathiger infestations and prevalent rate of that in cattle
Khayatnouri Mirhadi1*, Garedaghi Yagoob2 and Safarmashaei Saeid3
1Department of Pharmacology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2Department of Parasitology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3Young Researchers Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Accepted 04 May, 2019
Abstract
These days one of the real problems that cause the prejudice-economy in animal farms yearly is parasitic disease. For that reason and to prevent these problems the use of antiparasitic drugs is necessary. Ivermectin is a broad spectrum antiparasitic agent and is in different dosage forms such as injection, oral and pour-on. The aims of this study was to evaluate the efficacy of ivermectin pour-on administration against natural Nematodirus spathiger nematode infections in cattle and also to determine the prevalent rate of this parasite in Tabriz area. In this study, 120 heads of male and female hybrid cattle were used for LPG (larves per gram of feces) feces test. Willis method was applied for feces test and Stoll method was used for counting nematode larvae (LPG). After confirming worm contamination and counting N. spathiger nematode larves in feces in infected animals, ivermectin (0.5 mg/kg) pour-on was administrated. Feces test was repeated in 1, 7, 21 and 28 days after treatment. Results showed that total prevalence of N. spathiger nematode infections was 33.33% in cattle of Tabriz area. The effects of ivermectin pour-on on N. spathiger nematode were 13.37, 44.96, 83.69 and 99.1% in 1, 7, 21 and 28 days respectively. In conclusion, because the effect of this drug against N. spathiger nematode was >99% (p<0.05), cattle can use this drug in anti-parasitic programme. Of course, the effect of this drug on other nematodes and parasitic infections need more investigation.
Key words: Ivermectin, pouron administration, Nematodirus spathiger, cattle.
Khayatnouri Mirhadi*, Garedaghi Yagoob and Safarmashaei Saeid
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-006, June, 2019. © International Scholars Journals
Full Length Research Paper
In vitro evaluation of antimicrobial activity of methanolic extract from selected species of Cephalopods on clinical isolates
Pasiyappazham Ramasamy1, Namasivayam Subhapradha1, Alagiri Srinivasan2, Vairamani Shanmugam1, Jayalakshmi Krishnamoorthy1 and Annaian Shanmugam1*
1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University Parangipettai – 608 502, India.
2Department of Biophysics, All India Institute of Medical Science, New Delhi – 110029, India.
Accepted 10 March, 2019
Abstract
Bioactive substances from marine biota have been found useful as special tools in pharmacological and biomedical research. In the present study, the in vitro antimicrobial activity of crude methanolic extracts of six species of cephalopods (Sepia kobiensis, Sepiella inermis, Sepioteuthis lessoniana, Octopus aegina, Octopus aerolatus, Octopus dollfusi) from Cuddalore (Southeast coast of India) was studied. The antimicrobial activity was screened against 10 species of clinically isolated human pathogenic bacteria namely Vibrio chlolerae, Pseudomonas aeruginosa, Klebsiella pnemoniae, Vibrio alginolyticus, Staphylococcus aureus, Vibrio parehaemolyticus, Streptococcus sp., Streptococcus pnemoniae, Salmonella sp. and Escherichia coli and five fungal strains such as Alternaria alternata, Candida tropicalis, Pencillium italicum, Fusarium equisetii and Candida albicans. Different concentrations such as 25, 50, 75 and 100% were prepared and tested against the microbial strains for their inhibitory activities, using the disc diffusion method. The minimum inhibitory concentration (MIC) of methanolic extract of cephalopods ranged from 60 to 100 mg/ml. The results were discussed in the light of positive and negative control apart from the concentrations tested.
Keywords: Antimicrobial activity, cephalopods, minimum inhibitory concentration (MIC), pathogenic microorganisms.
Alagiri Srinivasan, Jayalakshmi Krishnamoorthy and Annaian Shanmugam*, Namasivayam Subhapradha, Vairamani Shanmugam, Pasiyappazham Ramasamy
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-006, June, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and evaluation of antibacterial activity of bacteriocin produced by Lactobacillus bulgaricus from yogurt
Maria Tufail1, Shahzad Hussain1*, Farnaz Malik2, Tahira Mirza2, Ghazala Parveen2, Shazia Shafaat2, Amina Wajid2, Rashid Mahmood2, Rafique A. Channa2 and Alia Sadiq4
1Hazara University, Mansehra, Pakistan.
2National Institute of Health, Islamabad-45500, Pakistan.
3Arid Agriculture University, Rawalpindi, Pakistan.
4Department of Microbiology Quaid-i-Azam University, Islamabad, Pakistan.
Accepted 09 March, 2019
Abstract
Lactic acid bacteria (LAB) commonly used in food, as starter cultures are known to produce antimicrobial substances such as bacteriocins and have great potential as food bio-preservatives. Yogurt is a probiotic dairy product having Lactobacillus bulgaricus as natural source for fermentation. The aim of this study was to determine the antimicrobial activity of bacteriocin, produced by Lactobacillus bulgaricus isolated from yogurt and tested against pathogenic organisms. The identification of the culture was based on characteristics of the strains of Lactobacillus spp. on the basis of microscopy (morphology), Gram straining, growth at 37 and 45°C, fermentation of different carbohydrate sources and growth in MRS broth. On the basis of all of the identification tests, one strain isolated from the yogurt was identified as L. bulgaricus. Culture supernatants were obtained from the sixty out of hundred isolates of Lactobacillus spp. exhibited varying degrees of inhibitory activity against strains of Bacillus subtilis, ATCC #6633, Escherichia coli, ATCC, #10536, Salmonella typhi, ATCC# 19430, Staphylococcus aureus, ATCC #6538, Vibrio cholerae, ATCC #25870. Specifically, Vibrio cholerae was found most sensitive to bacteriocin, exhibiting maximum zone of inhibition 18.3 mm.
Key words: Lactic acid bacteria (LAB), Lactobacillus bulgaricus, bacteriocin, yogurt, antimicrobial activity, Pakistan.
Farnaz Malik, Rafique A. Channa and Alia Sadiq, Shahzad Hussain*, Rashid Mahmood, Tahira Mirza, Maria Tufail, Ghazala Parveen, Shazia Shafaat, Amina Wajid
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-007, May, 2019. © International Scholars Journals
Full Length Research Paper
Incidence and antibiotic susceptibility profile of bacteria isolated from mobile phones of food handlers in Birnin Kebbi, Kebbi State, Nigeria
*Y.U. Dabai1, A.H. Kawo2 and R.M. Aliyu3
1Department of Public Health And Preventive Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Sokoto State, Nigeria.
2Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Sokoto State, Nigeria.
3Department of microbiology, Faculty of Science, Bayero University, Kano, Kano State, Nigeria.
Accepted 23 May, 2019
Abstract
This research was aimed to determine the incidence and antibiotic susceptibility profile of bacteria isolated from mobile phones of food handlers in Birnin Kebbi, Kebbi state Nigeria, using aseptic swab technique. Hundred swab samples from mobile phones of food handlers were collected. Bacteria Isolated were enumerated using pour plate method and overall mean aerobic mesophilic bacterial counts of 2.70x106CFU/ml was obtained. Bacterial isolates (n=111) belonging to three genera; S. aureus (n=88) Escherichia coli (n=19) and Salmonella spp (n=4) were identified. Antibiotic susceptibility profile of the isolates indicated that the highest sensitivity of 40mm against S. aureus was ciprofloxacin, 30mm against E. coli and Salmonella spp has 26mm. However, Salmonella spp was resistance to nitrofloxacin and chloramphenicol with 0.0mm. The highest zone of inhibition was recorded against ciprofloxacin with 30 and the least resistant drug was chloramphenicol with 0.0mm respectively. The findings are of public health concern since mobile phone may serve as a vehicle for transmission of various bacterial diseases. Since using mobile phones become day to day activities, food vendors and other common people should be sensitized to adhere to infection control measures, such as hand washing, to avoid possible cross-contamination between mobile phones and food items.
Keywords: Antibiotic susceptibility, S. aureus, Mobile phones, zone of inhibition, food handlers.
Full Length Research Paper
Incidence and antibiotic susceptibility profile of bacteria [h1] isolated from mobile phones of food handlers in Birnin Kebbi, Kebbi State, Nigeria
*Y.U. Dabai1, A.H. Kawo2 and R.M. Aliyu3
1Department of Public Health And Preventive Medicine, Faculty of Veterinary Medicine, U[h2] smanu Danfodiyo University, Sokoto, Sokoto State, Nigeria.
2Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Sokoto State, Nigeria.
3Department of microbiology, Faculty of Science, Bayero University, Kano, Kano State, Nigeria.
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Accepted 23 May, 2019 |
This research was aimed to determine the incidence and antibiotic susceptibility profile of bacteria isolated from mobile phones of food handlers in Birnin Kebbi, Kebbi state Nigeria, using aseptic swab technique. Hundred swab samples from mobile phones of food handlers were collected. Bacteria Isolated were enumerated using pour plate method and overall mean aerobic mesophilic bacterial counts of 2.70x106CFU/ml was obtained. Bacterial isolates (n=111) belonging to three genera; S. aureus (n=88) Escherichia coli (n=19) and Salmonella spp (n=4) were identified. Antibiotic susceptibility profile of the isolates indicated that the highest sensitivity of 40mm against S. aureus was ciprofloxacin, 30mm against E. coli and Salmonella spp has 26mm. However, Salmonella spp was resistance to nitrofloxacin and chloramphenicol with 0.0mm. The highest zone of inhibition was recorded against ciprofloxacin with 30 and the least resistant drug was chloramphenicol with 0.0mm respectively. The findings are of public health concern since mobile phone may serve as a vehicle for transmission of various bacterial diseases. Since using mobile phones become day to day activities, food vendors and other common people should be sensitized to adhere to infection control measures, such as hand washing, to avoid possible cross-contamination between mobile phones and food items.
Keywords: Antibiotic susceptibility, S. aureus, Mobile phones, zone of inhibition, food handlers.
A.H. Kawo and R.M. Aliyu, *Y.U. Dabai
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-009, May, 2019. © International Scholars Journals
Full Length Research Paper
Characterization of an exopolysaccharide-producing marine bacterium, isolate PSEUDOALTEROMONAS sp. AM
Al- Nahas, M. O.1, Darwish, M. M.1, Ali, A. E.2* and Amin, M. A.2
1Department of Chemistry of Natural and Microbial Products, National Research Center, Cairo, Egypt.
2Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Egypt.
Accepted 09 March, 2019
Abstract
A marine exopolysaccharide (EPS) producing bacterium was isolated from a sponge sample at about 16 m depth in red sea (Huraghada, Egypt). Phenotypic characterization demonstrated that, the bacterium is a gram negative rod motile by means of single polar flagellum, showing wide range of carbohydrate utilization. Phylogenetic analysis based on 16S Ribosomal deoxyribonucleic acid (rDNA) sequence revealed 99% homology with PSEUDOALTEROMONAS sp., accordingly the name PSEUDOALTEROMONAS sp. AM was proposed. Maximum EPS productivity was achieved after 7 days at pH 7, agitation of 150 rpm in a fermentation medium containing glucose, meat extract and 3% NaCl. The partially purified EPS had emulsifying activity about 78% and retained its stability for about 13 days. Extended emulsion stability for up to 22 days was shown by increasing the concentration of EPS from 0.01 to 0.1% w/v. The EPS elicited a marked antiviral activity against herpes simplex (HSV-I) and showed lysis of plasma clots comparable to pentosan sulphuric polyester as a standard. Preliminary characterization of EPS by complete acid hydrolysis then analysis on paper chromatography revealed the presence of a single brown spot equidistant with authentic glucose indicating that EPS is mainly composed of glucose units. Further investigations will be carried out to realize the full structure and chemical composition of the produced EPS and other possible biotechnological potential.
Key words: Exopolysaccharide, Pseudoalteromonas sp., emulsifying activity, antiviral activity, fibrinolytic activity.
Al- Nahas, A. E* and Amin, Ali , M. O, M. A, Darwish , M. M
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