ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-007, December, 2018. © International Scholars Journals
Full Length Research Paper
Pathogens change in spontaneous bacterial peritonitis patients with cirrhosis
Yanzi Gou1*, Fangxin Zhang1, Zhenbin Liang2, Xuefan Bai3, Lei Pan3, Wei Wang1, Bo Liu3, Jiuping Wang3 and Dingcheng Wang4
1Department of Gastroenterology, Lan Zhou General Hospital of People Liberation Army, Lanzhou, 730050, China.
2Surgery, 68302 Military Hospital, Shaanxi, 710000, China.
3Centre of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China.
4Clinical Laboratory Department, Tangdu Hospital, the Fourth Military Medical University Xi’an 710038, China.
Accepted 23 September, 2018
Abstract
The aim of this study was to determine the distribution of clinical microorganisms isolated from spontaneous bacterial peritonitis patients. Ascitic fluid was collected from these patients and cultured for pathogens. The patients were placed into three groups, Group A (01/1996 to 12/2000), B (01/2001 to 12/2005) and C (01/2006 to 06/2010) and the clinical data were compared among these groups. There was a significant difference in the ratio of pathogens (Gram- positive bacteria/ isolated pathogens, Gram-negative bacteria/ isolated pathogens) between groups A and C (P < 0.001). Spontaneous bacterial peritonitis patients with Gram-positive bacteria used significantly more antibiotics within 30 days compared to those with Gram-negative bacteria (P < 0.001) . In recent years, the types of isolated pathogens have significantly changed in Northern China. Such changes have also been observed in other countries and have been attributed to long-term antibiotic therapy and invasive procedures. Changes in the epidemiology of pathogens that cause spontaneous bacterial peritonitis must be monitored for optimal treatment.
Key words: Pathogens, gram-positive bacteria, spontaneous bacterial peritonitis, cirrhotic patients.
Bo Liu, Lei Pan, Jiuping Wang and Dingcheng Wang, Fangxin Zhang, Wei Wang, Yanzi Gou*, Zhenbin Liang, Xuefan Bai
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-006, December, 2018. © International Scholars Journals
Full Length Research Paper
A magnetic microparticle-based immunoassay for hepatitis C virus NS3 antigen
Li Xie1*, Yue-ping Guang2, Fang Liu1, Hong-bo Shi1, Li Yan1 and Jin-li Lou1
1Beijing You’an Hospital, Capital Medical University, Beijing 100069, China.
2University of Science and Technology Beijing, Beijing 100083, China.
Accepted 04 December, 2018
Abstract
In order to detect Hepatitis C virus NS3 antigen (HCAg-NS3) in human serum, we developed a magnetic microparticle (MMP) based immunoassay by using biotin-streptavidin amplification, MMP in combination with immunoassay. Results showed that the HCV NS3 monoclonal antibody had a high immobility rate (87.1%) on the surface of MMPs; the assay procedure required smaller volumes of reagents (only 20 l of immune magnetic bead); the detection could be completed within 30 min; this method could detect 10 pg/ml HCAg-NS3 (sensitivity: 5.2 pg/ml) and had favorable stability; the mean coefficient of variances (CVs) of intra-assay and inter-assay were 5.35 and 7.15%, respectively. The results of this method were also related to those in HCV RNA detection with a linear correlation coefficient of 0.570 (P < 0.01). These findings demonstrate this assay which has high specificity, sensitivity, precision and stability may be promising in clinical application.
Key words: Hepatitis C virus NS3 antigen, magnetic microparticle, biotin-streptavidin sytem (ABS), magnetic immunoassay.
Li Yan and Jin-li Lou, Fang Liu, Yue-ping Guang, Hong-bo Shi, Li Xie*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-008, December, 2018. © International Scholars Journals
Full Length Research Paper
Immunomodulatory effects of novel bifidobacterium and lactobacillus strains on murine macrophage cells
Jun Zhu1,2, Liang Zhao1,2, Huiyuan Guo1,2, Lu Jiang1,2 and Fazheng Ren1,2*
1College of Food Science and Nutritional Engineering, Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
2Beijing Higher Institution Engineering Research Center of Animal Product, Beijing 100083, China.
Accepted 21 December, 2018
Abstract
The principle purpose of this study was to investigate the immunomodulatory ability of three microbial strains isolated from Bama County centenarians in activating macrophages, and to characterize the effective component of these strains. A murine macrophage cell line, RAW264.7, was cultured in presence of intact bacterial cells, bacterial cell wall (CW) or cell free extract (CFE) . NO, IL-6, TNF-production and phagocytic activity of co-cultured macrophages were tested. Bifidobacterium adolescentis BBMN23, Bifidobacterium longum BBMN68 and Lactobacillus saliva Ren were demonstrated to enhance the activities of macrophages by increasing the phagocytic activity and NO, IL-6, TNF- production. The immunomodulatory activities of these microbial strains are mainly due to the CW fraction, although their CFE were also effective. The results of this study indicated that specific substrains of bifidobacterium and lactobacillus from guts of healthy centenarians (Bama County, China) may be of immunomodulatory value, and thus may have the potential to be used as probiotics.
Key words: Bifidobacterium, lactobacillus; RAW 264.7 cells, immunomodulatory activities, bacterial fractions.
Jun Zhu, Liang Zhao, Lu Jiang and Fazheng Ren*, Huiyuan Guo
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-004, December, 2018. © International Scholars Journals
Full Length Research Paper
A study on prostate specific antigen (PSA) with the ratio of free to total PSA
Chavan Atish1, Suman Diggi Prasad1, Rawal Mukesh1, Kandasamy Surendran2, Sivapatham Sundaresan3* and Thangarajan Thangapannerselvem4
1Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India- 603203, India.
2Central Laboratory, Department of Biochemistry, SRM Medical College Hospital and Research Centre, SRM University, Kattankulathur, Tamil Nadu, India- 603203, India.
3Department of Medical Research, SRM Medical College Hospital and Research Centre, SRM University, Kattankulathur, Tamil Nadu, India- 603203, India.
4Department of Biochemistry, SRM Medical College Hospital and Research Centre, SRM University, Kattankulathur, Tamil Nadu, India- 603203, India.
Accepted 10 September, 2018
Abstract
Prostate specific antigen (PSA) is a glycoprotein produced as a marker by prostate gland for prostate cancer, benign prostate hyperplasia and prostatitis. Study objective is to evaluate f/t PSA ratio to distinguish the Benign Prostate Hyperplasia (BPH) and prostate cancer patients in and around SRM University. To define the age specific reference ranges of PSA in control and test group at Chennai, India. Healthy men aged 40 - 75 years in and around SRM Medical College and Research Centre, Chennai, India were selected and grouped as control. Blood samples were collected from patients who attended Cancer Hospital, Adiyar underwent rectal examination revealed prostate enlargement. Results of our study showed that they were diagnosed as BPH and as Cancer, using PSA determination. The free to total PSA ratio were decreased significantly in cancer patients than BPH. PSA was increased linearly with age and observations were associated with the claims of National Academy of Clinical Biochemistry guideline reported that the clinical decisions limits should be decreased for younger patients (age below 50) and should be increased for older patients (age above 50). PSA should be used more appropriately to distinguish (BPH) and prostate cancer and to detect cancer prostate at an early stage. The age specific reference ranges and different forms of PSA have the potential to make serum PSA, a more discriminating tumor marker for detecting cancer prostate significantly in men.
Key words: Prostate specific antigen, free PSA prostate cancer, benign prostate hyperplasia, free to total PSA ratio, complex PSA, percentage of free PSA.
Chavan Atish, Rawal Mukesh, Kandasamy Surendran, Suman Diggi Prasad, Sivapatham Sundaresan* and Thangarajan Thangapannerselvem
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-005, December, 2018. © International Scholars Journals
Full Length Research Paper
Effect of egusi melon oil on lecithin: Cholesterol acyltransferase activity in rats fed a cholesterol diet
G. C. Ojieh1, G. O. Eidangbe1 and O. M. Oluba2*
1Department of Medical Biochemistry, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.
2Department of Biochemistry, University of Benin, Benin-City, Nigeria.
Accepted 29 October, 2018
Abstract
The effect of feeding egusi melon oil as a supplement to a cholesterol-based diet on serum lecithin: cholesterol acyltransferase activity of rats was evaluated. The rats were divided into two groups designated: control and test respectively. Rats in the test group were fed 5% cholesterol diet supplemented with 5% egusi melon oil while the control rats received 5% cholesterol diet without egusi melon oil. After 6 weeks of diet feeding, the enzyme activity was decreased significantly (p < 0.05) in the egusi melon oil- fed rats compared with the control. The test group also showed relative significant decreases in the serum levels cholesteryl ester and lysolecithin (p < 0.05) and increase levels of lecithin (p < 0.05). Significant decreases (p < 0.05) were also observed in serum total and free cholesterol in the egusi melon oil treated group compared to the control group. The implications of these results are discussed with respect to hypercholesterolemia.
Key words: Egusi melon oil, cholesterol diet, cholesteryl ester, LCAT, supplementation.
G. C. Ojieh, G. O. Eidangbe and O. M. Oluba*
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-004, December, 2018. © International Scholars Journals
Full Length Research Paper
Effects of soaking and boiling and autoclaving on the nutritional quality of Mucuna flagellipes (“ukpo”)
E. A. Udensi1, N. U. Arisa2* and E. Ikpa1
1Department of Food Technology, Bells University of Technology, Ota, Ogun State, Nigeria.
2Department of Food Science and Technology Abia State University Uturu, Abia State, Nigeria.
Accepted 30 October, 2018
Abstract
The effect of soaking and boiling, and autoclaving on nutritional factors (proximate and mineral) and anti-nutritional qualities of the legume, Mucuna flagellipes were studied. Batches of seeds were soaked for 6, 12, 18 and 24 h in distilled water at room temperature, then boiled in water for 30, 45, 60 or 90 min respectively. Another batch of M. flagellipes was autoclaved for different duration of 30, 45, 90 and 120 min respectively. Results showed that soaking followed by boiling produced products with lower crude fibre content (10% for soaking for 24 h followed by boiling for 90 min). However it increased the carbohydrates. Autoclaving resulted in products with lower mineral contents (1042.5 mg/100 g phosphorous for autoclaving for 120 min.
Key words: Legumes, Mucuna flagellipes, nutritional factors, soaking, boiling, autoclaving.
E. A. Udensi, N. U. Arisa* and E. Ikpa
Page: 1 - 4