Advanced Journal of Microbiology Research

ISSN 2736-1756

Table of Contents 2019

Short Communication

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-003, February, 2019. © International Scholars Journals

Short Communication

Effect of Tai Chi exercise on older people with nephritis

Cao Yun1 and Niu Aijun2*

1Department of Sport of Zunyi Normal University, Zunyi, Guizhou 563002, China.

2Wushu Department, Guangzhou Sport Universtiy, Guangzhou 510500, China.

Accepted 19 January, 2019

Abstract

To evaluate the effect of Tai Chi exercise on older people with nephritis, 124 older people with nephritis were asked to practice Tai Chi exercise for 40 min once every day. Results showed that Tai Chi” quan exercise significantly decrease high-shear whole blood viscosity, low-shear whole blood viscosity, plasma viscosity, erythrocyte sedimentation rate (ESR), hematocrit (HCT), usea nitrogen, creatinine and creatinine scavenging rate during 12 months of practice. This indicates that Tai Chi exercise is beneficial to prevent and delay nephritis deterioration.

Key words: Tai Chi, nephritis, old people, hematocrit (HCT).

Niu Aijun*, Cao Yun

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-005, February, 2019. © International Scholars Journals

Full Length Research Paper

Potential of Beauveria bassiana as a biological control agent against two important and destructive stored pests’ C. maculatus and S. granarius adults

Golnaz Shams1*, Mohammad Hassan Safaralizadeh2, Sohrab Imani1, Mahmoud Shojai1 and Shahram Aramideh2

1Department of Entomology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2Department of Plant Protection, Agricultural Faculty, Urmia University, Urmia, West Azerbaijan, Iran.

Accepted 03 Jaunary, 2019

Abstract

This research was carried out to evaluate the suppressive efficacy of entomopathogenic fungi Beauveria bassiana against adults of Callosobruchus maculatus (F.) and Sitophilus granarius (L.) on stored grains in darkness (27±2°C and 65±5% RH) . The insects were inoculated by following immersion in five aqueous suspensions with concentrations determined with preliminary tests and compared with untreated wheat and cowpea as control. Probit analysis showed that the lowest LT 50 values in suspensions with highest concentrations (2.3 × 107 conidia ml-1) were 6.63 and 10.45 days for C. maculatus and S. granarius, respectively. On the other hand, the LC50 values on day 9 post-treatment were 3.17 × 106 and 6.08 × 107 con.ml-1 for C. maculatus and S. granarius, respectively. Comparison of Lc50, LT 50 values and mortalities indicated that in both assays, B. bassiana was consistently more virulent for C. maculatus than S. granarius. The overall results showed that these two pests were controlled successfully with entomopathogenic fungi Beauveria bassiana.

Key words: Callosobruchus maculatus, Beauveria bassiana, Sitophilus granarius, stored grain.

Mohammad Hassan Safaralizadeh, Sohrab Imani, Mahmoud Shojai and Shahram Aramideh, Golnaz Shams*

Page: 1 - 5

Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals

Full Length Research Paper

Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)

Ayman S. Daba1, Ghada A. Youssef2*, Sanaa S. Kabeil1 and Elsayed E. Hafez1

1Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt.

2Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.

Accepted 19 January, 2019

Abstract

Successful utilization of Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366) mushroom as a type of edible locally isolated mushroom in Egypt at the Mushroom Research Center (Mubarak City for Scientific Research and Technology Applications), to produce extensive hydrolyzing cellulase complex enzymes. This hydrolysis was approached in submerged culture supplemented with avicel PH101 as a substrate for endo-,exoglucanase production. The avicel concentration 6% yielded the maximum enzyme activities (2.46, 1.80 U/ml) for both endo-and exoglucanase activities on basal medium at 27°C, initial pH value of 5.5 for 12 days on rotary shaker (180 rpm) incubation period. Cellulase enzyme was amplified using specific PCR and the amplicone was cloned using TOPO TA cloning vector. The cellulolytic activity of the recombinant protein was examined and high activity was obtained compared with the standard ones. The avicel was used as a sole carbon source in the fermentation medium and the results revealed that, avicel induced the cellulolytic activity of the examined organism compared with those grown on medium deficient of avicel.

Key words: Pleurotus ostreatus, mushroom, avicel, recombinant cellulolytic enzymes.

Ayman S. Daba, Ghada A. Youssef*, Sanaa S. Kabeil and Elsayed E. Hafez

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals

Full Length Research Paper

Production and oil-emulsion formulation of Cadophora malorum and Alternaria jacinthicola, two biocontrol agents against Water Hyacinth (Eichhornia crassipes)

Karim Dagno1,2*, Rachid Lahlali3, Mamourou Diourté2 and Haïssam M. Jijakli1

1Unité de Phytopathologie. Gembloux Agro-Bio Tech, Passage des Déportés 2, B-5030 Gembloux, Université de Liège - Belgique.

2Programme Sorgho, Centre Régional de Recherche Agronomique de Sotuba. BP : 262, Bamako, Mali.

3Agriculture and Agric-Food Canada, Saskatoon Research Centre, 107 Science Place, S7N 0X2 - Canada.

Accepted 18 January, 2019

Abstract

Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores of these agents on locally available substrates (Water Hyacinth, powdered paddy rice chaff, wheat semolina) was assessed with a view to mass production. The C. malorum isolate sporulated best on Water Hyacinth (4.08 × 107 spores ml-1), followed by wheat (1.06 ×107 spores ml-1), whereas A. jacinthicola produced more spores on paddy rice chaff and wheat (0.24 x 107 spores ml-1). The severity of the damage caused by each pathogen was evaluated in the greenhouse and in the field. Under both greenhouse and field conditions, the biocontrol efficacy of the fungal isolates was improved with (unrefined) Carapa procera (L.) oil or (refined) palm oil, supplemented with soybean lecithin and Tween 20. When such a formulation was used, the incubation time was 4 to 5 days in the greenhouse and 7 to 9 days on the field, and the damage severity (DS) recorded 6 weeks after treatment varied from 87.02 to 93. 13% in the greenhouse and from 59.11 to 63.00% in the field. For unformulated C. malorum and A. jacinthicola respectively, the incubation times were longer and the DS values were only 22.11 and 29. 05% in the greenhouse and 12.05 and 15.15% on the field. Our results highlight good substrates for mass production of these mycoherbicides and demonstrate the ability of vegetable oil formulations to improve their efficacy.

Key words: Alternaria jacinthicola, biocontrol, Cadophora malorum, oil formulation, Water Hyacinth.

Karim Dagno*, Mamourou Diourté and Haïssam M. Jijakli, Rachid Lahlali

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-005, February, 2019. © International Scholars Journals 

Full Length Research Paper

Microbiological assessment of mutton intended for export from Elkadaro export slaughter house, Sudan

Ibrahim Mohammed Elhassan1, Atif Elamin Abdelgadir2* and Abedalaziz Eltayeb Ibrahim2

1Ministry of Animal Resources and Fisheries, Khartoum, Sudan.

2Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum, Sudan.

Accepted 08 January, 2019

Abstract

The study is planned to evaluate the hygienic quality of mutton intended for export from Elkadaro slaughter house on basis of surface bacterial contamination. Seventy five samples were collected during five visits, 15 samples in each visit (five samples for each: slaughter hall, slaughter house chiller and the refrigerated vehicle at Airport after unloading). A metallic triangle was used as a template from which swabs were taken. The results of the bacterial counts revealed relatively high counts (ranges between 1 × 103 - 6 × 106 CFU/ cm 2), but without critical contamination levels according to the cut-off point for fresh meat (106 CFU/ cm2) and chilled meat (107 CFU/ cm2) except in the last visit (2 × 106 CFU/ cm2).

Key words: Sudan, mutton, slaughter house, export.

Atif Elamin Abdelgadir* and Abedalaziz Eltayeb Ibrahim, Ibrahim Mohammed Elhassan

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals

Full Length Research Paper

Antibacterial activity of several Malaysian leaves extracts on the spoilage bacteria of yellow alkaline noodles

Teza Alfindo Rosyid1, Roselina Karim1*, Noranizan Mohd Adzahan1 and Farinazleen Mohd Ghazali2

1Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, My, 43400, Malaysia.

2Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, My, 43400, Malaysia.

Accepted 11 January, 2019

Abstract

Yellow alkaline noodle (YAN) is very susceptible to spoilage and has a short shelf life due to the high moisture content. This study was conducted to isolate and identify spoilage bacteria of YAN in an attempt to apply local plant extracts that possess antibacterial activity in extending the shelf life of YAN. Thirty colonies were isolated from spoiled YAN and were identified using the Biolog GEN III system. Eight bacteria which consisted of five Gram-positive (GP) and three Gram-negative (GN) (Bacillus pumilus, Clavibacter agropyri, Corynebacterium urealyticum, Corynebacterium jeikeium, Enterobacter cloaceae, Pseudomonas aeruginosa, Serratia marcescens and Staphylococcus sciuri) were identified. E. cloaceae and S. sciuri were the most abundant bacteria on YAN with percentage values of 23 and 26%, respectively. The antibacterial activities of ethanol and water extracts of six types of leaves (Centella asiatica, Jasminum sambac, Pereskia bleo, Cosmos caudatus caudatus, Murraya koenigii, and Melicope lunu) against all the identified bacteria were studied. The ethanol extracts of M. koenigii are most effective extract that possess the highest antibacterial activity against all the eight spoilage bacteria studied.

Key words: Yellow alkaline noodle, spoilage bacteria, plant extracts, antibacterial activity.

Teza Alfindo Rosyid, Roselina Karim*, Noranizan Mohd Adzahan and Farinazleen Mohd Ghazali

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