ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-013, April, 2020. © International Scholars Journals
Full Length Research Paper
Comparative analysis on the curing effect of acridine orange (mutagen) on beta - lactamase producing Staphylococus aureus, from bovine and human origin
Onyenwe, N. E.1, Mbata T. I.2* and Adeleke, O. E.1
1Department of Pharmaceutical Microbiology, University of Ibadan, Oyo state, Nigeria.
2Department of Microbiology, Federal Polytechnic Nekede, Owerri, Nigeria.
Accepted 17 October, 2019
Abstract
The effect of the mutagen (acridine orange) on the multi-resistant antibiotics beta lactamase producing Staphylococcus aureus strain of bovine and human origin was investigated and tested individually using iodiometric (cell supervision) method for the level of beta-lactamase produced by each strain and antibiotic – sensitivity screening using single disc agar diffusion method. Analyses revealed that 80% of the isolates from bovine origin produced beta-lactamase while only 20% showed absence of the enzyme, while 68% of the human isolates produced beta-lactamase while only 32% showed absence of the enzyme sensitivity to cefotaxime, cefuroxime and ceftriaxine were observed in both strains of bovine and human origin. At high concentrations of the mutagen (250 µg/ml), zones of inhibitions were low for penicillin, amoxicillin and augmentine at reference MIC’s of 0.25 µg/ml, while the cephalosporins recorded high zones of inhibition at 0.5 and 1.0 µg/ml reference MIC’s in all the strains at 250 and 200 µg/ml of the concentration of the mutagen (acridine orange). The resistance bovine strains producing beta-lactamase resisted curing with acridine orange than the human strains of S. aureus.
Key words: Acridine orange, Staphylococcus aureus, beta-lactamase, iodometric.
N. E., Mbata T. I.* and Adeleke, O. E., Onyenwe
Page: 1 - 13
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-005, April, 2020. © International Scholars Journals
Full Length Research Paper
Determination of the effect of light and salt concentrations on Aphanocapsa algal population
Ifeanyi, V. O.1*, B. N. Anyanwu2, J. N.Ogbulie2, R. N. Nwabueze2, W. Ekezie1 and O. S. Lawal1
1Microbiology Department, Anambra State University, Uli. Anambra State, Nigeria.
2Industrial Microbiology Department, Federal University of Technology, Owerri.Imo State, Nigeria.
Accepted 19 May, 2019
Abstract
The research was on the determination of the effect of light and salt concentrations on Aphanocapsa algal population. Microbiological standards were observed in the isolation of Aphanocapsa sp from water sample collected from Shell Petroleum flow station. The effect of 5,000, 3500 and 2,000 lux light intensities and 10 g/L through 50 g/L salt concentrations on the growth and proliferation of Aphanocapsa sp was determined by a spectrophotometer at 340 nm. At Day 9, the algal species gave the highest optical density of 0.495 nm at 5000 lux. The optimum salinity on the algal growth was 10 g/L and this gave 1.23 nm optical density at Day 9. The control gave 0.45 nm on Day 9. This research monitored the effect various light and salt concentrations had on Aphanocapsa sp growth and the optimum light intensity and salt concentration were identified.
Key words: Algae, environmental parameters, light intensity, salt concentration, growth, population.
J. N.Ogbulie, Ifeanyi , V. O.*, W. Ekezie and O. S. Lawal, B. N. Anyanwu, R. N. Nwabueze
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-006, April, 2020. © International Scholars Journals
Full Length Research Paper
Detection of ecotone environment based on satellite and field techniques (A case study; Northern Alborz, Iran)
Karim Solaimani* and Fatemeh Shokrian
GIS & RS Centre, University of Agriculture and Natural Resources, P. O. Box 737, Sari-Iran.
Accepted 10 May, 2019
Abstract
Environmental change and socio-economic pressure are expected to have significant impacts on Northern Alborz vegetation, particularly along ecotone such as the treeline. Remote sensing may be well suited to monitoring recent changes across the treeline because it captures integrated changes of all vegetation life forms over large spatial extents. This research examines treeline vegetation composition and change along the Nojmeh treeline using a high resolution, ETM+ 2000. In this paper we investigate the use of satellite data to produce a classification of a treeline ecotone in Northern Alborz Mountains which has supported the field investigation as ground truth data collected in the summers from 2008 to 2010. The maximum correlation is related to the Bands 3 and 4 of Landsat ETM+ with shrubs canopy cover that indicated in r of 0.34 and 0.37 respectively. The maximum rate of correlation in respect to the indices were recognized for the vegetation cover of moisture stress index (MSI) for forbs cover (r= 0.62). The ratio vegetation index (RVI) for grasses cover was indicated (r= 0.53). In conclusion, the results are specified that the ETM+ sensor is the significant data with the elevated competence to discrimination of ecotone using vegetation indices.
Key words: ETM+, classification, ecotone, treeline, Alborz, Iran.
Karim Solaimani*, Fatemeh Shokrian
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-007, April, 2020. © International Scholars Journals
Full Length Research Paper
Biofilm formation ability of Paenibacillus polymyxa and Paenibacillus macerans and their inhibitory effect against tomato bacterial wilt
Bin Li1, Rongrong Yu2, Qiaomei Tang1, Ting Su1, Xiaoling Chen1, Bo Zhu1, Yanli Wang3, Guanlin Xie1 and Guochang Sun3*
1State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
2Zhejiang University of Technology, Hangzhou 310032, China.
3State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Accepted 17 October, 2019
Abstract
The potential of biofilm formation of 16 Paenibacillus strains and their inhibitory effect against bacterial wilt of tomato seedlings were examined in this study. The crystal violet assay indicated that all strains of Paenibacillus except strain MB02-1202 formed biofilm after 96 and 144 h of incubation while there was not significant difference in biofilm formation between strains of Paenibacillus polymyxa and strains of Paenibacillus macerans. However, the increase level of biofilm formation was associated with the increase of the incubation time and the initial inoculum density. In addition, all Paenibacillus strains except strain MB02-428 reduced wilt incidence in tomato seedlings inoculated with Ralstonia solanacearum while the cell numbers of R. solanacearum in rhizosphere soil was reduced by all Paenibacillus strains compared to the pathogen control. In general, most strains of Paenibacillus were able to both form biofilm and protect tomato seedlings from bacterial wilt, indicating that biofilm formation may play an important role in the biocontrol of Paenibacillus. This is first study regarding the relationship between in vitro biofilm formation ability of Paenibacillus strains and their inhibitory activity against R. solanacearum.
Key words: Biofilm, Paenibacillus, Ralstonia solanacearum, tomato.
Ting Su, Bo Zhu, Rongrong Yu, Bin Li, Guanlin Xie and Guochang Sun*, Qiaomei Tang, Yanli Wang, Xiaoling Chen
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-007, April, 2020. © International Scholars Journals
Full Length Research Paper
Copepod parasites of gills of 14 teleost fish species caught in the gulf of Annaba (Algeria)
Boualleg C.1, Kaouachi N.1, Seridi M.1, Ternango S.2 and Bensouilah M. A.1
1Laboratoire Ecobiologie des Milieux Marins et Littoraux, Faculté des Sciences. Université Badji Mokhtar Annaba. BP: 12 El- Hadjar. Annaba. 23000. Algérie.
2Laboratoire Parasites et Ecosystèmes Méditerranéens, Faculté des Sciences et Techniques, Université de Corse, BP 52, 20250 Corte, France.
Accepted 07 January, 2020
Abstract
A parasitic copepods study of Algerian teleost fish, report 25 copepod species belonging to eight families harvested from the gills of 14 fish species. The analysis of species richness according to some ecological, biological and taxonomical variables (Diet, Displacement, Way of life, Family) of the whole of hosts are reported in this study, the differences between mean specific richness (MSR) of all variables is statically significant according to Kruskal-Walis test using sigma stat software. Parasitic specificity is discussed, infestation parameters were described.
Key words: Gulf of Annaba, copepods, species richness, parasitic indices, parasitic ecology, teleost fishes.
Seridi M, Ternango S and Bensouilah M. A., Kaouachi N, Boualleg C
Page: 1 - 8
Review
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-005, April, 2020. © International Scholars Journals
Review
A review of the prevalence of emerging Bartonellosis in humans and animals in China
Congbin Yao, Caixia Zhu, XueYin Pan, Xiuguo Hua and Congli Yuan*
Laboratory of Zoonosis and Comparative Medicine, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, People’s Republic of China.
Accepted 20 November, 2019
Abstract
The genus Bartonella is comprised of 20 species and subspecies, in which 10 species are responsible for human infections. The identification of the causative agent of cat-scratch disease, bacillary angiomatosis, urban trench fever, and Carrion's disease has raised the emerging medical importance of Bartonella spp. This article provides an overview of bartonellosis with emphasis on its prevalence in China.
Key words: Bartonella, cat-scratch disease, wild animal, prevalence.
XueYin Pan, Caixia Zhu, Xiuguo Hua and Congli Yuan*, Congbin Yao
Page: 1 - 5