ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII T30C
Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2
1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.
2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.
Accepted 03 March, 2019
Abstract
Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.
Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
Quick isolation and characterization for the confirmation of a novel Bacillus thuringiensis strains from chicken manure samples
Suguna Muniady1, Xavier Rathinam1 and Sreeramanan Subramaniam2*
1AIMST University, Bukit Air Nasi, Jalan Bedong, 08100, Kedah, Malaysia.
2School of Biological Sciences, Universiti Sains Malaysia, Gelugor, 11800 Penang, Malaysia.
Accepted 25 January, 2019
Abstract
Bacillus thuringiensis (Berliner) (Bt) based pesticides has become the greatest successes among the microbial pesticides. This research was focused on the isolation and characterization of Bt from chicken manure samples as a comprehensive effort to contribute to the establishment of native Bt strains from various types of habitats in Malaysia. Initially, selection was done mainly based on colony morphology, Gram staining and phase contrast microscopy. Total of 85 colonies were identified and isolated based on colony morphology, in which off-white, matte and creamy appearance showed the characteristics of the genus Bacillus. The number reduced to 35 colonies after Gram staining was done. Subsequently, total of 8 isolates were confirmed to be B. thuringiensis after Coomassie Brilliant Blue staining was carried out. The presence of B. thuringiensis strain was confirmed when a blue cap -like structure and a darkly stained oval structure, insecticidal crystal proteins (ICP) appeared in the sporulation phase. An isolate was chosen out of the 8 isolates in order to generate the growth curve of B. thuringiensis bacteria. The similarity of the aspects above confirms that the selected isolate was a Bt strain. Isolates were further characterized by SDS-PAGE analysis to determine the molecular masses of the Bt proteins. These isolates produced two major ranges of polypeptides, the Cry protein which corresponds to a range of 70-150kDa and the Cyt protein profile which corresponds to the range of 18-40kDa. The protein profile obtained correlates the strains to the typical Bt strain. A total of 6 isolate yielded SDS-PAGE products that demonstrated major protein bands of approximately 135kDa and 140kDa, possibly encoded by the genes cryI and cryIV, respectively.
Key words: Bacillus thuringiensis, chicken manure, protein analysis.
Xavier Rathinam and Sreeramanan Subramaniam*, Suguna Muniady
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Toxicity of essential oil from Artemisia argyi against Oryzaephilus surinamensis (Linnaeus) (Coleoptera: Silvanidae)
Jianhua Lü*, Cunrong Wu and Ya Shi
School of Food Science and Technology, Henan University of Technology, Zhengzhou 450052, China.
Accepted 22 March, 2019
Abstract
The repellent, fumigant effect and contact toxicity of essential oil extracted from Artemisia argyi (Asteraceae: Artemisia) plant against Oryzaephilus surinamensis (Linnaeus) (Coleoptera: Silvanidae) was investigated. The A. argyi oil exhibited strong repellent, fumigant effect and contact toxicity against O. surinamensis which progressively increased with increased exposure dosage. Repellency percentage of A. argyi oil against O. surinamensis adults reached IV grade after 48 h exposure period at the dosage of 0.40 µl/cm2, the corrected percentage mortality for fumigant toxicity reached more than 97% at the dosage of 160 µl/l air, and the current population inhibition percentage (CPI) and F1 progeny population inhibition percentage (PPI) reached 77.38 and 96.06% at the dosage of 0.80 µl/g, respectively. The results suggest that the A. argyi oil has great potential for effectively controlling O. surinamensis.
Key words: Natural product, Artemisia argyi, toxicity, Oryzaephilus surinamensis, stored grain insect.
Jianhua Lü*, Cunrong Wu and Ya Shi
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-006, April, 2019. © International Scholars Journals
Full Length Research Paper
Survey beta lactamase production and resistance pattern into beta lactame antibiotics in Bacillus cereus strain isolated from staff hands and hospital environment in Iran
Shilla Jalalpoor
Department of Microbiology, Islamic Azad University Shahreza Branch, Membership of Young Researchers Club, Isfahan, Islamic Republic of Iran. E-mail: [email protected].
Accepted 24 March, 2019
Abstract
Hospital infection is one of the important agent morbidity and mortality in patient. Bacillus cereus is one of the bacteria that cause nosocomial infection. Hospital surfaces and staff hands have important to creation nosocomial infections. Hospital surfaces have potentional capacity to carry bacteria and staff hands are the most reason for the transmission of bacteria to hospital. The present study was performed at one tertiary care hospitals in Isfahan, Iran. During a 30 month period. According to statistical formula, randomly selected 328 samples were needed for study, that from this samples 164 samples was environmental and 164 samples was clinical. Environmental and staff hands samples respectively were randomly selected from different ward of hospital and fingerprint technique, then samples cultured on Blood agar and EMB and were examined by Kirby Bauer Antibiogram and Acidimetric test. Of 328 samples, 21 samples was B. cereus. According to the result the prevalence of B. cereus was 6.4% (p=0.56) so, antibiotic resistance in B. cereus strain for Penicillin, Cefotaxime and Ampicillin were respectively 81, 47.6 and 19%. According to Acidimetric test 100% of isolated B. cereus strains from environmental, and 92.30% from staff hands product –Lactamase. Result demonstrate high prevalence of resistance to –Lactamase antibiotics B. cereus strain. One of the reason to create antibiotic resistant in bacteria is to increase contact of Bacteria.
Key words: Bacillus cereus, –lactamase, antibiotic resistance, nosocomial infection, staff hands, hospital surfaces.
Shilla Jalalpoor
Page: 1 - 6
Review
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-009, April, 2019. © International Scholars Journals
Review
Advances in microbial heterologous production of flavonoids
Fangchuan Du1,2, Fangkai Zhang1,2, Feifei Chen1,3, Anming Wang1*, Qiuyan Wang1, Xiaopu Yin1 and Shuling Wang1
1Research Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou 311121, China.
2College of Biological and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
3College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Accepted 21 February, 2019
Abstract
Flavonoids are important plant specific secondary metabolites that are synthesized from the general phenylpropanoid pathway and have many pharmaceutical and nutraceutical functions. Separation of pure flavonoids from plants material is very difficult and chemical syntheses of flavonoids require extreme reaction conditions and toxic chemicals. However, in the past decades, engineered microbes are becoming increasingly important as recombinant production platforms. Cultivation of the recombinant strains in rich medium offers another choice for the production of flavonoids. In the present review, we cover the main achievements that the multi-gene pathway of phenylpropanoid is introduced as microorganisms to heterologously produce flavonoids, analyze the key factors affecting heterologous production of flavonoids in microbes, and discuss the new research prospect.
Key words: Flavonoids, metabolic engineering, natural products, synthetic biology.
Xiaopu Yin and Shuling Wang, Fangchuan Du, Qiuyan Wang, Fangkai Zhang, Anming Wang*, Feifei Chen
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-003, April, 2019. © International Scholars Journals
Full Length Research Paper
Helicobacter pylori and CagAantibodies in hyperemesis gravidarum (HG)
Fatemeh Abbasalizadeh1*, Shamsi Abbasalizadeh1, Mohammad Reza Bonyadi2 and Parvin Bastani1
1Women’s Reproductive Health Research Center Tabriz University (Medical Sciences), Tabriz, Iran.
2Drug Applied Research Center, Tabriz University (Medical Sciences), Tabriz, Iran.
Accepted 19 March, 2019
Abstract
In order to determine whether infection with Helicobacter pylori and CagA strain is associated with hyperemesis gravidarum (HG), a study was conducted in Al- Zahra obstetrics and gynecology hospital in Tabriz, Iran between May 2007 and February 2008. Forty-four (44) pregnant women with the diagnosis of HG and forty-four (44) normal pregnant women of matched gestational age were included in this prospective study. Serum H. pylori immunoglobulin G antibody titer and CagAantibodies were measured for both groups of women. There was no difference in seropositivity of H. pylori antibody between subjects with hyperemesis when compared with controls. The prevalence of H. pylori infection with CagA + gene was significantly higher among control group when compared with study group. HG seems not to be associated with H. pylori infection. The results of this study suggested higher levels of CagA H. pylori infection in control groups.
Key words: Helicobacter pylori, hyperemesis gravidarum, CagA + gene.
Shamsi Abbasalizadeh, Mohammad Reza Bonyadi and Parvin Bastani, Fatemeh Abbasalizadeh*
Page: 1 - 3