ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Histopathology of the gill and liver tissues of the African catfish Clarias gariepinus exposed to lead
OLOJO, E. A. A. 1*, OLURIN, K. B., 1MBAKA, G. and OLUWEMIMO, A. D.1
1Department of Biological Sciences, Olabisi Onabanjo University, P. M. B. 2002, Ago-Iwoye, Ogun State, Nigeria.
2Department of Anatomy, College of Basic Medical Sciences, Olabisi Onabanjo University, P.M.B. 2002, Ago-Iwoye, Ogun State, Nigeria.
Accepted 21 July, 2004
Abstract
An investigation on the effect of the heavy metal, lead (Pb) on the gill and liver of the African catfish Clarias gariepinus was carried out in the laboratory. One hundred and sixty (160) fingerlings of the fish were exposed to continuous exposure to sub-lethal concentrations (0.006 mg/l and 0.008 mg/l) of lead for a period of three weeks. The liver and gill of fish were removed every 9 days for histological examination. The results showed that the degree of distortion of the gills and liver was proportional to the exposure periods and concentration of the metals was found to be dose and time dependent.
Key words: Lead, Clarias gariepinus, histopathology, gill, liver.
K. B., OLURIN , E. A. A*, A. D, OLOJO , G. and OLUWEMIMO, MBAKA
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Export papaya post -harvest protection by fungicides and the problems of the maximal limit of residues
DEMBELE Ardjouma1*, Traore Sory Karim2, KONE Mamadou2, COULIBALY Dotana Ténébé3
1LABORATOIRE Central d’Agrochimie et d’Ecotoxicologie, LANADA 04 BP 612 Abidjan 04 COTE D’IVOIRE.
2 UFR des Sciences et Gestion de l’environnement, Université d’Abobo - Adjamé 02 B.P. 801 Abidjan 02/COTE D’IVOIRE.
3UFR des Sciences de la Nature/Protection des Végétaux et de l’environnement, Université d’Abobo - Adjamé, 02 B.P. 801 Abidjan 02/COTE D’IVOIRE.
Accepted 28 September, 2004
Abstract
The post-harvest maturity of papaya fruits is influenced by several environmental factors including temperature, light and ventilation of the surrounding wall. The maturity, presence and attacks or rots, and the accumulation of fungicide residues in the papaya was evaluated on papaya fruits treated with different fungicide. Thiabendazole-treated fruits did not rot 21 days after treatment and this fungicide was the most satisfactory with detected residues lower than the European Union’s 2000/48/EC guideline.
Key words: Post-harvest, papaya, fungicides, phytotoxic, thiabendazole, residues maximal limit.
KONE Mamadou and COULIBALY Dotana Ténébé, Traore Sory Karim, DEMBELE Ardjouma*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Retention of-carotene in frozen carrots under varying conditions of temperature and time of storage
Debjani Dutta, Utpal Raychaudhuri, Runu Chakraborty*
Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata – 700032, India.
Accepted 15 August, 2004
Abstract
Carrots were blanched and stored in a freezer to study the retention of - carotene. Retention was found to be better in 3 min blanched samples than in 5 min ones. There was an increase in -carotene content in carrots after 5 days at all storage temperatures: 0, –8, –14, and –18oC. Decrease was found to be insignificant compared to the initial value even after 80 days of storage time at –18oC and for 3 min blanch time.
Key words: Carrots, -carotene, blanching, freezing, activation energy, storage time.
Utpal Raychaudhuri, Debjani Dutta, Runu Chakraborty*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Stability of yam starch gels during processing
N’Guessan Georges Amani1*, Alphonse Kamenan1 Agnès Rolland-Sabaté2, and Paul Colonna2
1Universityof Abobo-adjamé, UFR/STA, 02 BP 801, Abidjan, Côte d'Ivoire.
2INRA, rue de la Géraudière, BP 71627, 44316 Nantes, France.
Accepted 5 December, 2004
Abstract
To evaluate their aptitude to be used as functional ingredients, twenty one varieties of yam starches of Côte d’Ivoire were submitted to different technological stress such as high temperature treatment, long term freezing and refrigeration, high speed shearing and acidic treatment, in comparison with commercial modified starches. The gel of “kangba” starch (D. Cayenensis-rotundata) is the most stable during thermal processing. The cultivar “Daminangba” ( D. alata) which present the clearest gel (63 % of clarity) is also the most stable during refrigeration with a low syneresis (26%) at 4°C.The “Esculenta 7” cultivar (D. esculenta) shows the weakest value of syneresis at -20°C. The gel of D. dumetorum species is the strongest under acidic condition with 8% of viscosity decrease from pH 7 to pH 3, whereas the “Bodo” cultivar ( D. alata) gel shows good resistance to shearing with 31% fall of viscosity from 160 rpm to 900 rpm on the RVA. The “Sopèrè”, “Lopka” and Kponan cultivars (D. Cayenensis–rotundata) present the strongest viscosities in all the technological treatments.
Key words: Yam starch, modified starch, functional properties, gel stability.
N’Guessan Georges Amani*, and Paul Colonna, Alphonse Kamenan Agnès Rolland-Sabaté
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Effect of Nauclea latifolia leaves aqueous extracts on blood glucose levels of normal and alloxan-induced diabetic rats
Gidado, A., Ameh, D.A.* and Atawodi, S.E.
Department of Biochemistry Ahmadu Bello University, Zaria Zaria, Nigeria.
Accepted 17 November, 2004
Abstract
In Northern Nigeria, different parts of Nauclea latifolia (family: Rubiaceae) are prescribed as remedy for diabetes mellitus. This study was designed to evaluate the hypoglycemic property of aqueous extract of the leaves of the plant in normal and alloxan-induced diabetic rats. The aqueous extracts at (200 mg/kg bw.) significantly lowered glucose levels (p<0.05) of the diabetic rats by 45% within 4 h but showed no similar effect in normoglycaemic rats. This finding suggest that aqueous extract of the leaves of N. latifolia posses hypoglycaemic activity to warrant further detailed study to elucidate its therapeutic, toxicological and phytochemical properties.
Key words: Nauclea latifolia, hypoglycaemic activity, alloxan-induced diabetes.
S.E. , D.A.* and Atawodi, A. , Gidado , Ameh
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis
Vipul Gohel, Pranav Vyas and H.S. Chhatpar*
Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, Vadodara - 390 002, Gujarat, India.
Accepted 15 November, 2004
Abstract
A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were separately incorporated in chitin agar plates. After running polyacrylamide gel electrophoresis, the gel was transferred onto chitin agar plate containing different dyes for the activity staining. Thin layer of acetate buffer (0.2 M, pH 5) was pored on the gel, which helps faster diffusion of the enzyme from gel onto the plate. After incubation of about 7 h, bands of chitinase were visible by daylight or UV light. The method is very sensitive since it can detect even 0.5 units of chitinase. Thus, this method is sensitive, rapid, user-friendly, reliable and cost effective.
Key words: Activity staining, chitinase, dyes, sensitivity, stability.
Vipul Gohel, Pranav Vyas and H.S. Chhatpar*
Page: 1 - 10