ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Short Communication
A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)
D. Puchooa
Faculty of Agriculture, University of Mauritius, Réduit, Mauritius. E-mail: [email protected].
Accepted 16 March 2004
Abstract
A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.
Key words: Litchi chinensis, genomic DNA isolation.
D. Puchooa
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
A Study on the use of carrot juice in the tissue culture of Daucus carota
D. Puchooa* and R. Ramburn
Faculty of Agriculture, University of Mauritius, Réduit, Mauritius.
Accepted 30 March 2004
Abstract
Effects of using carrot juice in the in vitro growth of Daucus carota were investigated. Callus was the only form of growth obtained after five weeks in culture. The increase in fresh weight, dry weight and moisture content of the explants with increasing concentration of carrot juice in the medium was much lower when compared to either the cytokinin benzyladenine (BA) and/or the auxin picloram. Spectrophotometric and chromatographic analyses revealed the presence of both indole-3-acetic acid (IAA) and abscissic acid (ABA) in the carrot juice, while gibberellic acid was absent.
Key words: Carrot juice, auxin, abscissic acid.
R. Ramburn, D. Puchooa*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
Potential of cassava flour as a gelling agent in media for plant tissue cultures
Moses F. A. Maliro1,2* and Grace Lameck1
1Bunda College of Agriculture, University of Malawi, P.O. Box 219, Lilongwe, Malawi. Telephone: 265 1 277 420, Fax: 265 1 277 361
2Joint Centre for Crop Innovation JCCI), Private bag 260, Horsham, Victoria 3401, Australia. Telephone: 61 3 5362 2367 (office) and 61 3 5382 0386 (Home), Fax: 61 3 5362 2388.
Accepted 24 March 2004
Abstract
The potential of a tissue culture medium gelled with cassava flour to support shoot proliferation of stem nodal sections of Uapaca kirkiana and Faidherbia albida was studied. A two factorial experiment was conducted with the first factor as type of gelling agent (80 g/L cassava flour; 80 g/L cassava flour mixed with 3.5 g/L agar; and 7 g/L agar) while the second factor was two plant species. Explants were obtained from 8-months old U. kirkiana and F. albida in vitro raised seedlings. Data included number of shoots and height and analysis of variance was performed. Number of shoots proliferating and shoot height from cultures on medium gelled with cassava flour was the lowest. This was attributed to the degradation of the cassava flour gel after two weeks. Performance of the cassava flour mixed with 3.5 g/L agar gel was better than agar alone. The stability of cassava flour as a gelling agent can therefore be improved by mixing with some agar.
Key words: Gelling agent, cassava flour, agar, shoot proliferation.
Moses F. A. Maliro*, Grace Lameck
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
A morphometric analysis of the genus Ficus Linn. (moraceae)
Mubo, A. Sonibare1*, Adeniyi, A. Jayeola2 and Adeyemi Egunyomi2
1Department of Biological Sciences, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
2Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.
Accepted 21 December 2003
Abstract
Foliar parameters of Ficus in Nigeria were subjected to quantitative analysis. The morphometric analysis is based on ten quantitative parameters of the leaves of species. Principal compound analysis produced six groups whose characters are described. Highly significant positive correlation exists between leaf length and leaf width, leaf length and lamina length, leaf length and petiole length, lamina length and lamina width. Negative correlation was observed between leaf width and leaf length/width ratio, petiole length and fruit length/petiole length ratio. The groups that emerged compared well with existing traditional classification with some sub-sectional discrepancies.
Key words: Ficus, morphometric analysis, numerical classification.
Mubo , A. Sonibare*, A. Jayeola and Adeyemi Egunyomi, Adeniyi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
Nutrients requirements in biological industrial wastewater treatment
Bashaar Y. Ammary
Water and Environmental Engineering Department, Balqa Applied University, Huson College, P.O.Box 50, Huson 21510, JORDAN. Phone: +962-2-7010400, Fax: +962-2-7010379. E-mail: [email protected].
Accepted 30 March, 2004
Abstract
Wastewaters from olive mills and pulp and paper mill industries in Jordan have been characterized and treated using laboratory scale anaerobic and aerobic sequencing batch reactors, respectively. Nutrient requirements for these two industrial wastewaters were found to be less than what is usually reported in the literature for C:N:P ratio of 100:5:1 for aerobic treatment and 250:5:1 for anaerobic treatment. This was ascribed to the low biomass observed yield coefficients and relatively low removal efficiencies in these wastewaters. It was found that for anaerobic treatment of olive mills wastewater COD:N:P ratio of about 900:5:1.7 was able to achieve more than 80% COD removal. The observed biomass yield was about 0.06 kg VSS per kg of COD degraded. For extended aeration aerobic treatment of pulp and paper mill wastewater COD:N:P ratio of about 170:5:1.5 was able to achieve more than 75% COD removal. The observed biomass yield was about 0.31 kg VSS per kg of COD degraded. In both these wastewaters nutrients were not added. A simple formula is introduced to calculate nutrient requirements based on removal efficiency and observed biomass yield coefficient.
Key words: Olive mill wastewater, anaerobic treatment, aerobic treatment, sequencing batch reactor, biomass yield, nutrient requirement.
Bashaar Y. Ammary
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2004
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2004 International Scholars Journals
Full Length Research Paper
Direct genetic transformation of Hibiscus sabdariffa L.
Yaye Kène Gassama-Dia*, Djibril Sané and Mansor Ndoye
Laboratoire de Biotechnologies Département de biologie végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, B.P. 5005, Dakar, Sénégal.
Accepted 1 April, 2004
Abstract
Transgenic Hibiscus sabdariffa plants have been produced by a tissue culture independent method using Agrobacterium tumefasciens transformation procedure. Embryo axes of mature seeds with one cotyledon excised were infected by immersion in a suspension of Agrobacterium LBA 4404 strain culture that carries pBal plasmid with β-glucuronidase p35SGUSINT and plant selectable marker Neomycin Phospho-Transferase gene (nptII). Following a 24 h co-cultivation with Agrobacterium strain and decontamination with cefotaxime, embryos were grown on soil rite containing MS medium added with a killer concentration of kanamycin (100 µg/ml) during 4 weeks at room conditions and thereafter transferred to greenhouse. 54.3% of the seedlings grew well on the selective medium; 68% of the explants excised from putative transformed plants were found to be GUS positive. After 60 days evaluation point, the assessment of the transformation by PCR revealed that H. sabdariffa line tested, carried the nptII gene.
Key words: Hibiscus sabdariffa, genetic transformation.
Yaye Kène Gassama-Dia*, Djibril Sané and Mansor Ndoye
Page: 1 - 10