ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Effect of oil-pulling on dental caries causing bacteria
T. Durai Anand*, C. Pothiraj, R. M. Gopinath and B. Kayalvizhi
PG Department of Microbiology, V. H. N. S. N. College, Virudhunagar- 626 001, India.
Accepted 12 February, 2012
Abstract
The effect of oil-pulling on the reduction of total count of bacteria was determined. There was a remarkable reduction in the total count of bacteria. The process of oil- pulling reduced the susceptibility of a host to dental caries. The in-vitro antibacterial activity of sesame oil against dental caries causing bacteria was determined. Streptococcus mutans and Lactobacillus acidophilus were found to be moderately sensitive to the sesame oil.
Key words: Antibacterial activity, Streptococcus mutans and Lactobacillus acidophilus.
T. Durai Anand*, C. Pothiraj, R. M. Gopinath and B. Kayalvizhi
Page: 1 - 10
Short Communication
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Short Communication
Growth and survival of Salmonella zanzibar in juice and salami stored under refrigerated and room temperature
Chatti Abdelwaheb*, Lamouri Imen and Landoulsi Ahmed
Département des Sciences de la vie, Faculté des Sciences de Bizerte, Zarzouna 7021, Tunisie.
Unité de Biochimie des Lipides et interaction des macromolécules en Biologie, 03/UR/0902.
Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Zarzouna 7021.
Accepted 7 February, 2012
Abstract
The vehicles usually involved in salmonellosis outbreaks are meat, eggs, poultry and milk. Recently, Salmonella outbreaks were reported in some vegetables such as tomatoes and unpasteurized juice. After inoculation of Salmonella zanzibar onto food samples, growth and survival of S. zanzibar in orange juice and salami were monitored. Our results showed that S. zanzibar is able to grow and survive in orange juice and also in salami. However, growth in salami was more important than in the case of orange juice. Storage at 4°C caused a difference in the survival curve when compared to samples (also juice and salami) incubated at room temperature. The present study demonstrated that orange juice and salami can provide a favorable environment for survival and growth of S. zanzibar in spite of their low pH values.
Key words: Salmonella, orange juice, salami, survival, refrigeration.
Lamouri Imen and Landoulsi Ahmed, Chatti Abdelwaheb*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Phytochemical and antibacterial screening of Anogeissus leiocarpus against some microorganisms associated with infectious wounds
A. Mann*, Y. Yahaya, A. Banso and G. O. Ajayi
Department of Science Laboratory Technology, The Federal Polytechnic, P. M. B 55, Bida, Niger State. Nigeria.
Accepted 29 June, 2012
Abstract
Ethanolic extracts of the leaf, stem and root bark and the combination of the three parts of Anogeissus leiocarpus were investigated for in vitro antibacterial activity against clinical isolates of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using agar diffusion techniques. The extracts of the plant parts showed higher antibacterial activity against S. aureus (15.80 ± 0.85) than other tested organisms. The plant parts generally were found to contain important bioactive substances such as glycosides, phenols, tannins, saponins, alkaloids, steroids, ellagic acids and anthraquinones. These agents may be responsible for the antibacterial activity of this plant.
Keywords: Anogeissus leiocarpus, clinical isolates, antibacterial activity, bioactive.
Y. Yahaya, A. Banso and G. O. Ajayi, A. Mann*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Quantitative survey and anti-microbial effect of Piliostigma thonnigii and Khaya ivorensis leaves on traditional dry-yam
Babajide, J. M * and Atanda, O.O
Department of Food Science and Technology, University of Agriculture, P. O. Box 52, Alabata-UNAAB, Abeokuta, Nigeria.
Accepted 20 March, 2012
Abstract
A survey was conducted to determine the quantity of local preservatives (‘Abafe’ Piliostigma thonnigii and ‘Agehu’ Khaya ivorensis leaves) used for parboiling yam and the anti-microbial effect of the leaves on dry- yam ‘Gbodo’. Although, the study revealed that 23 g of abafe, 13 g of agehu and a combination of 12.8 g of abafe and 12.3 g of agehu will be required for 1 kg of yam, there were wide ranges between the quantities of yam as well as the quantities of local preservatives used by the processors. Futhermore, there were reduction in the microbial loads of samples parboiled with the leaves (singly or combined) respectively, immediately after processing and after 3 months of storage compared with the control. Thus, P. thonnigii leaves had bactericidal while K. ivorensis leaves had fungicidal effect on the microorganisms encountered during processing and storage of Gbodo.
Key words: Quantitative survey, anti-microbial, Piliostigma thonningii, Khaya ivorensis, dry-yam.
O.O , J. M * and Atanda, Babajide
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Immunoglobulin profile of some Nigerians with Schistosoma haematobium infection
O. P. G. Nmorsi1*, N. C. D. Ukwandu2, C. Isaac1, A. O. Egwunyenga3 and N. H. Olague1
1Tropical Diseases Research Unit, Department of Zoology, Ambrose Ali University, Ekpoma, Nigeria.
2Department of Medical Microbiology, Ambrose Ali University, Ekpoma, Nigeria.
3Department of Zoology, Delta State University, Abraka, Nigeria.
Accepted 13 May, 2012
Abstract
The preliminary investigation revealed the prevalence of 138(46.9%) out of 294 volunteers screened for the ova of Schistosoma haematobium in their urine samples. Of these, 84(28.6%) had light infection (50 ova/10 ml urine), while 54(18.4%) had heavy infection (> 50 ova/10 ml urine). This difference was statistically significant at ( 2 = 6.52, p > 0.05). The mean immunoglobulin status were as follow: IgE (2141.6 ± 143.7 ng/dL), IgG (13.6 ± 3.53 mg/dL), IgA (3.72 ± 0.149 mg/dL), IgM (2.82 ± 0.48 mg/dL) and IgD (0.12 ± 0.04 mg/dL) . The relationship between the IgM, IgE and the intensities of infection were positively correlated (r = 0.27 and r = 0.65, respectively) . IgG, IgA and IgD showed negative correlation with the intensities of infection (r = -0.65, r = -0.39 and r = - 0.18, respectively). IgG and IgA can be used as markers of light infection, while IgM and IgE can be used as markers for heavy infection. We deduced that the levels of IgG, IgA and IgM, which were depleted in the infected volunteers, compared to the control subjects, which lacked significant protective effects in these infected volunteers. These low levels of IgA, IgG and IgM and high level of IgE may be involved in maintenance of S. haematobium infection in our study area.
Key words: IgA, IgD, IgE, IgG, IgM, Schistosoma haematobium, Nigerians
N. C. D. Ukwandu, A. O. Egwunyenga and N. H. Olague, C. Isaac, O. P. G. Nmorsi*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Potential commercialization of a microbial medium formulated from industrial food waste
Zvidzai C.* 1, Muzhinji N.1, Chidzvondo F.1, Mundembe R.2, and Sithole-Niang I.1
1Biochemistry Department, University of Zimbabwe, Box MP167, Mt. Pleasant, Harare, Zimbabwe.
2Bindura University of Science and Education, P. Bag 1020, Bindura, Zimbabwe.
Accepted 31 April, 2012
Abstract
A microbial medium, designated DYSP medium, was produced from food wastes that included defatted soya, clear beer spent yeast, potato solid waste and opaque beer spent grains. Various combinations of the food wastes were used for the media formulations and evaluated for extent of supporting microbial growth of pure cultures. A 3.0 M sodium hydroxide hydrolysis followed by a neutralization process using concentrated hydrochloric acid has been established and standardized for the preparation of the medium. A dry pulverized medium was produced that could be reconstituted in distilled water (dH2O) without settleable solids.The formulated DYSP medium supported the growth of Escherichia coli, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Aspergillus niger and Saccharomyces cerevisiae. The composition of the medium was 35.45% protein, 5.7% nitrogen, 56.6% ash, 8.6% moisture and 0.012 mg/ml of total reducing sugar. The pH of the DYSP broth was 6.6 when reconstituted in distilled water. Traditional classical microbiological studies demonstrated that the test cultures could grow and retain normal phenotypic and morphological properties when cultured on the formulated medium.The DYSP medium containing ampicillin, isopropyl ß-D-1-thiogalactopyranoside and 5-bromo-4-chloro-3-indolyl-ß-D-galactoside proved to be an equally alternative medium in molecular biology for selection and screening E. coli TG1 cells transformed with pUC18 plasmid. Other preliminary biotechnological results showed that the formulated medium could form a base for studying and optimizing the production of penicillin by P. chrysogenum.
Key words: Food wastes, chemical hydrolysis culturing medium, fermentation and molecular biology.
Zvidzai C.*, Chidzvondo F., and Sithole-Niang I., Mundembe R., Muzhinji N.
Page: 1 - 10