ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-005, May, 2020. © International Scholars Journals
Full Length Research Paper
Morphological, pathological and molecular variability of Colletotrichum capsici causing anthracnose of chilli in the North-east of Thailand
Aphidech Sangdee*, Sarawut Sachan and Surasak Khankhum
Department of Biology, Faculty of Science, Mahasarakham University, Thailand 44150, Thailand.
Accepted 23 January, 2020
Abstract
Anthracnose disease is one of the major economic constraints to chilli production in tropical and subtropical regions. Ten isolates of Colletotrichum capsici causing chilli anthracnose were collected from 10 provinces in the northeast of Thailand. The isolates were evaluated for their morphological and cultural characteristics, pathogenic variability on chilli fruits and genetic characterization using random amplified polymorphic DNA (RAPD-PCR). Based on the morphological traits and cultural characteristics of the C. capsici populations, 10 isolates were categorized into six groups. These were designated, respectively, as CC-I, CC-II, CC-III, CC-IV, CC-V and CC-VI. In Potato Dextrose Agar culture, most of the isolates produced cottony colonies. However, differences were obtained in colony color, shape and size of conidia. Based on the effect of carbendazim, 10 isolates were classified into two groups designated as highly resistant group (<40% inhibition) and highly sensitive group (>90% inhibition). Three virulence degrees of 10 isolates on chilli fruits were evaluated. Molecular polymorphism generated by RAPD confirmed the variation of the different isolates and they were grouped into two clusters. However, morphological, pathological and RAPD grouping of isolates suggested no correlation among the test isolates.
Key words: Colletotrichum capsici, morphological and cultural characteristics, pathogenic variability, random amplified polymorphic DNA (RAPD).
Aphidech Sangdee*, Sarawut Sachan and Surasak Khankhum
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-005, May, 2020. © International Scholars Journals
Full Length Research Paper
Antibacterial effects of probiotics isolated from yoghurts against some common bacterial pathogens
Hami Kaboosi
Department of Food Science, Ayatollah Amoli branch, Islamic Azad University, Amol, Iran. E-mail: [email protected]. Tel: (+98) 912-572-2900. Fax: (+98) 121-255-2782.
Accepted 15 January, 2020
Abstract
According to the definition of probiotics by the Food and Agriculture Organization of the United Nations/World Health Organization, “probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host”. Microorganisms that are probiotics in humans include Enterococci, Bifidobacteria and lactic acid bacteria, such as Lactobacilli, Lactococci and Streptococci. This research was conducted to determine the presence of antibacterial effects among the probiotics isolated from different bioyoghurts against some common bacterial pathogens. Lactobacillus sp., Streptococcus sp. and Bifidobacterium sp. from yoghurts containing probiotics were isolated and examined for their antibacterial effects against Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. The modified agar overlay method was used for determination of the presence of antibacterial effects among the isolated probiotics. Results showed the presence of antibacterial effects among the probiotics that were isolated from bioyoghurts. The spectrum of their antibacterial effects varied against the selected pathogen. Antibacterial effects are one of the most important selection criteria for probiotics, and the verified antibacterial activity of the probiotics supports the development of these functional foods as a key to the improvement of health in the consuming public.
Key words: Probiotics, bioyoghurts, Lactobacillus spp., Bifidobacterium spp., antibacterial effects.
Hami Kaboosi
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-006, May, 2020. © International Scholars Journals
Full Length Research Paper
Effect of ultraviolet, electromagnetic radiation subtype C (UV-C) dose on biofilm formation by Pseudomonas aeruginosa
Myriam BEN SAID*, Sana KHEFACHA, Lobna MAALEJ, Imen DALY and Abdennaceur HASSEN
Water Treatment and Recycling Laboratory (LTRE); Water Research and Technologies Centre (CERTE). Borj-Cedria, BP 273, Tunis 8020, Tunisia.
Accepted 25 August, 2019
Abstract
Pseudomonas aeruginosa is a commonly found nosocomial opportunistic pathogen that is characterized by its ability to form biofilm to protect itself from various external pressures. Biofilms are problematic in particular food industry sectors and in water distribution systems. In this study, we propose to explore the impact of UVC dose on biofilm production by P. aeruginosa strains. The biofilms densities were assessed by crystal violet assay. A relationship between UVC exposed dose and biofilm density was examined by establishment of new model linking between UVC dose and physiology bacteria response concerning biofilm production (UVC dose/ biofilm production).
Key words: UVC dose, biofilm, P. aeruginosa, quorum sensing, RecA, model.
Imen DALY and Abdennaceur HASSEN, Lobna MAALEJ, Sana KHEFACHA, Myriam BEN SAID*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-009, May, 2020. © International Scholars Journals
Full Length Research Paper
Nanotechnology approach on anticancer and antiviral properties of Consolida Sp.: The study of dipole moment and nuclear magnetic resonance (NMR)
M. Monajjemi1*, R. Hasani2, R. Rasoolzadeh2 and F. Mollaamin3
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Science and Research Branch, Islamic Azad University, Tehran, Iran.
3Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran.
Accepted 09 September, 2019
Abstract
14-O-demethyledelboxine is one of the most important alkaloids in Consolida species. It is toxic and has many effects in body. In this article, we used dipole moment and nuclear magnetic resonance (NMR) to describe this molecule properties. Nanoparameters and chemical shift calculations, geometry optimization and energies have been performed with ab initio method at HF/Sto-3G, B3Lyp/6-31G and B3Lyp/6-31G* levels with magnetic properties of the gauge-including atomic orbital method. According to this study this molecule has polarity, therefore it can highly react with microtubules. So it is recommended that more study in this molecule and the groups can be bound to evaluate these characters so it could be useful. Dipole moment calculations approve ab initio methods according to these results.
Key words: 14-o-demethyldelboxine, nuclear magnetic resonance (NMR), B3lyp, isotropy, anisotropy.
M. Monajjemi*, R. Hasani, R. Rasoolzadeh and F. Mollaamin
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-006, May, 2020. © International Scholars Journals
Full Length Research Paper
Comparison between the conventional and modern techniques used for identification of Mycobacterium tuberculosis complex
Moussa I. M.1*, Kh. F. Mohamed2, Marwah Mohamed3, Nasr E. A.3, Atef M. Shibl4, Mounir M. Salem-Bekhit4 and Hatem M. E.2
1Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460 - Riyadh11451, King Saudi Arabia.
2Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
3Department of Bacterial Diagnostic Products Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
4College of Pharmacy, King Saud University, P. O. Box: 2457, Riyadh 11451, Saudi Arabia.
Accepted 12 October, 2019
Abstract
Polymerase chain reaction (PCR) are rapid and simple means for the differentiation of members of Mycobacterium tuberculosis complex, especially Mycobacterium bovis and M. tuberculosis, where it is important to distinguish between zoonotic sources (cattle and unpasteurized dairy products) and human sources of tubercle disease. This study is aimed to evaluate the recent technique such as PCR and (BACTEC MGIT 960 TM system) for diagnosis of M. tuberculosis complex among cattle in Egypt. 1180 cattle were examined during the period of 2008 to 2010 by single intradermal tuberculin test. 29 animals (2.46%) were positive reactors, the results of isolation and identification using conventional culture method Lowenstein-Jensen medium were 22 mycobacterial isolates (75.9%), 20 (68.97%) M. bovis and 2 (6.9%) unidentified slow grower}. The recovery rate of BACTEC MGIT 960TM system was 82.8%, while in case of Lowenstein-Jensen medium was 75.9%. The mean time for detection of Mycobacterium was 17.8 ± 0.9 days and 46.5 ± 0.4 days for BACTEC MGIT 960TM system and Lowenstein-Jensen medium, respectively. While the contamination rate with BACTEC MGIT 960TM system was 6.9 and 10.3% in Lowenstein-Jensen medium. PCR technique in the present study could differentiates between M. bovis and M. tuberculosis within few h rather than the long period required for the biochemical identification tests. Therefore, the use of PCR in the diagnosis of Mycobacterium in clinical samples is as rapid, more reliable, sensitive and specific techniques and can be used for large scale screening of Mycobacterium in areas where the disease is still a public health hazard as in Egypt.
Key words: Bovine tuberculosis, tuberculin test, Lowenstein-Jensen medium, BACTEC system, PCR.
Mounir M. Salem-Bekhit and Hatem M. E, Atef M. Shibl, Kh. F. Mohamed, Nasr E. A, Moussa I. M*, Marwah Mohamed
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-010, April, 2020. © International Scholars Journals
Full Length Research Paper
Study of most prevalent wheat seed-borne mycoflora and its effect on seed nutritional value
Abdul Rehman1, Kishwar Sultana1, Nisar Minhas3, Muhammad Gulfraz1, Ghazala Kaukab Raja1* and Zahid Anwar2
1Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
2Department of Biochemistry Nawaz Sharif Medical College, University of Gujarat, Pakistan.
3Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
Accepted 25 May, 2019
Abstract
To study most prevalent wheat seed-borne mycoflora and its effect on the nutritional value, freshly harvested three and six months old stored wheat grains were collected from various parts of Pakistan. For isolation of seed-borne fungi, agar plate method was found more effective as compared to standard blotter method. Alternaria alternata was found to be the most prevalent fungus. Chemical analysis of seeds clearly showed a decrease in the carbohydrate, fats and ash contents of stored wheat grains as compared to the freshly harvested seeds with no effect on total proteins. The growth of A. alternata on seeds during storage might have resulted in low nutritional contents.
Key words: Mycoflora, Alternaria alternata, seed-borne fungi, post-harvest storage, pathogens.
Kishwar Sultana, Ghazala Kaukab Raja* and Zahid Anwar, Muhammad Gulfraz, Abdul Rehman, Nisar Minhas
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