ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-008, March, 2019. © International Scholars Journals
Full Length Research Paper
The mRNA quantitative analysis, prokaryotic expression and structure prediction of hepatocarcinogenesis related gene idh3 under AFB1 stress
Zhuang Zhenhong, Zhang Feng, Li Yanyun, Yuan Jun, Yang Yanling, Lin Ling and Wang Shihua*
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, and College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Accepted 19 February, 2019
Abstract
By differential proteomics analysis, it was found that the expression level of IDH3 (Isocitrate dehydrogenase 3 ) in mouse liver was significantly increased under the stress of Aflatoxin B1. To validate the result of differential proteomics analysis, fluorescence quantitative PCR was used to detect the changing trend of idh3 mRNA volume induced by Aflatoxin B1 in mouse liver. The result showed that the expression volume of idh3 mRNA showed an increasing trend with the increase of Aflatoxin B1 concentration, which corresponded with the result of differential proteomics analysis. The protokaryon expression vector for idh3 was constructed in the study with pET28a as a recipient plasmid. The expression vector (pET28a-idh3 ) was used to transform BL21, after which the positive expression strain (Escherichia coli BL21/pET28a-idh3 ) was induced to express with 100 mmol/L IPTG under 28°C for 4 h, and the prokaryotic expression product of IDH3 was successfully detected by SDS-PAGE electrophoresis. The molecule structure of IDH3 was predicted by bioinformatics analysis, and the results showed that the total number of negatively and positively charged residues were 42 and 39, respectively. Five hydrohpobic domains were predicted in the protein, and its average hydrophobicity was -0.069. There was 43% - helix and 20% -pleated sheet in the molecule, and the tertiary structure of IDH3 was constituted by 12 - helices and 12 -pleated sheets. Based on the results of bioinformatics analysis, the fragments of 1- 17 and 112-123 residues of IDH3 were selected as candidates for further effective polyclonal antibody preparation. The results of this study paved way for further exploration of the role of idh3 in the process of liver carceration induced by Aflatoxin B1.
Key words: IDH3 , aflatoxin B1, liver, canceration, bioinformatics analysis.
Yuan Jun, Yang Yanling, Zhang Feng, Lin Ling and Wang Shihua*, Zhuang Zhenhong, Li Yanyun
Research Article
Cytokine genes expression in mice hepatocytes during malaria infection
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Cytokine genes expression in mice hepatocytes during malaria infection
Saad Alkahtani1*, AL-Farraj S. A.1, Saud A. Alarifi2, AL-Eissa Mohammed saad3 and Al-Dahmash B.4
1Department of Biology, Teachers College, King Saud University, Riyadh, Saudi Arabia.
2Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia.
3Department of Biology, Science College, Hail University, Hail, Saudi Arabia.
4Department of Medical Laboratory, College of Health Sciences, King Saud University, Riyadh, Saudi Arabia.
Accepted 11 February, 2019
Abstract
The aim of this study was to investigate the inflammation genes (IL-1 , IL-6, TNF - and iNos) responses to Plasmodium chabaudi malaria in the liver of female C57BL/6 hepatocytes via mRNA expression due to infection with P. chabaudi at different time points. Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1, 4 and 8 respectively). RT-PCR was used to quantify liver inflammation genes. The levels of IL-1 , IL- 6 and TNF were significantly increased at days 1 and 8. The total iNos were significantly increased at all days after infection. In conclusion, present data has shown that infection with P. chabaudi stimulated infalammation genes in the liver. Thus, we suggest the implication of oxidative stress due to outcome of malaria in mice hepatocytes according to its natural function need to be confirmed with a larger number of samples to be used as a reliable inflammation detection method.
Key words: Inflammation, malaria, mice, liver.
AL-Eissa Mohammed saad and Al-Dahmash B, Saad Alkahtani*, AL-Farraj S. A, Saud A. Alarifi
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
An investigation on antibiotic resistance of Neisseria gonorrhoeae isolated from gonorrheal patients in Zahedan, Iran from 2007 to 2010
Mohammad Bokaeian, Mazhar Iqbal Qureshi, Soroush Dabiri and Amir Hossein Mohagheghi Fard*
Research Center for Zahedan Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, I. R. Iran.
Accepted 09 January, 2019
Abstract
Present study was aimed to determine antibiotic susceptibility and penicillinase production by Neisseria gonorrhoeae strains isolated from gonorrheal patients in Zahedan (south-east Iran). In a descriptive study from 2007 to 2010, 400 suspected patients were studied by history review, medical examination, Gram staining and culture in Thayer-Martin medium. Antibiotic susceptibility test of isolated strains was done by disk diffusion method and penicillinase test in penicillin resistant isolates by aciodometric method. The culture of 77(19.2%) of gonorrheal patients were positive. The resistance rate against applied antibiotics was as follow: penicillin (79.2%), ciprofloxacin (53.2%), ceftriaxone (3.8%), spectinomycin (2.5%), cefixim (12.9%), co-trimoxazole (93.5%), tetracycline (88.3%) and gentamicin (29.8%). In the meantime, 83.1% of penicillin resistant isolates produced penicillinase enzyme. Ceftriaxone, spectinomycin and cefixime are the sole antibiotics that could be considered as selective drugs. Quinolones which were regarded as an effective group of antibiotics until recently, haven lost their efficacy. Resistance against other antibiotics is rapidly growing, thus, conducting experimental tests and determination of minimum inhibitory concentration and clinical trial studies at fixed intervals can contribute to diagnosis of resistance of gonococci and rapid and successful treatment of their infections.
Key words: Neisseria gonorrhoeae, gonorrhea, penicillinase (β-lactamase).
Mohammad Bokaeian, Mazhar Iqbal Qureshi, Soroush Dabiri and Amir Hossein Mohagheghi Fard*
Research Article
Mycobacterium tuberculosis TLR2 agonists LprA, LM and Man-LAM induce notch1 and socs3 transcription
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Mycobacterium tuberculosis TLR2 agonists LprA, LM and Man-LAM induce notch1 and socs3 transcription
Erika Nahomy Marino-Marmolejo1, Citlalli Tornez-Benítez2, Vania Elvira Bonifaz-Peña3, Mabel Guzmán-Rodríguez3, Hugo Enrique López-González1, Abel Gutiérrez-Ortega1, Leticia Santos3* and Mario Alberto Flores-Valdez1*
1Centro de Investigación y Asistencia en Tecnología y diseño del Estado de Jalisco, A.C., Guadalajara, Jalisco, 44270 México.
2Laboratorio Estatal de Salud Pública de Guerrero “Galo Soberón y Parra”, Acapulco, Guerrero, México, 39715.
3División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C. San Luis Potosí, San Luis Potosí, 78216 México.
Accepted 20 January, 2019
Abstract
Mycobacterium tuberculosis employs a number of strategies to subvert host signaling events, leading to its persistence within macrophages. Upon infection, Mycobacterium bovis BCG induce the expression of suppressor of cytokine signaling 3 (socs3), in a Toll-like receptor 2 (TLR2)-Notch1-dependent manner. Purified phosphatidyl inositol di-mannosides (a TLR2 agonist) act as an inducer for the Notch1-socs3 pathway. This prompted us to analyze other TLR2 agonists seeking for additional molecules that may affect this pathway. We found that lipoprotein LprA, as well as glycolipids lipomannan (LM), and mannose- capped lipoarabinomannan (ManLAM) treatment of murine macrophages resulted in stimulation of notch1 and socs3 transcription.
Key words: Mycobacterium tuberculosis, Toll-like receptor 2 (TLR2), notch1, socs3, innate immunity.
Citlalli Tornez-BenÃtez, Vania Elvira Bonifaz-Peña, Leticia Santos* and Mario Alberto Flores-, Abel Gutiérrez-Ortega, Mabel Guzmán-RodrÃguez, Erika Nahomy Marino-Marmolejo, Hugo Enrique López-González
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Contamination of cattle carcasses by Escherichia coli shiga like toxin with high antimicrobials resistance
Everlon Cid Rigobelo1*, Renato Pariz Maluta2, Clarissa Araújo Borges2, Lívia Gerbasi Beraldo2, Manoel Victor Franco3, Lemos Sirlei Aparecida Maestá1 and Fernando Antonio de Ávila4
1Campus Experimental de Dracena- Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Brazil.
2Programa de Pós-Graduação em Microbiologia Agropecuária Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Jaboticabal Brazil.
3Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal. Departamento de Biologia Aplicada a Agropecuária, Brazil.
4Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Departamento de Patologia Veterinária, Brazil.
Accepted 15 February, 2019
Abstract
During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be by Escherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between March 2008 and August at 2009. A total of 120 STEC were isolated. All isolates were confirmed as being E. coli by their biochemical analysis and submitted to polymerase chain reaction (PCR) for detection of stx, eae and ehly genes. No strains was isolated being carriers of ehly gene. The number of isolates carriers of eae gene were 48/120. The most frequent resistance was seen against cephalothin (84.0%), streptomycin (45.0%), nalidixic acid (42.0%) and tetracycline (20.0%). Multidrug resistance (MDR) to three or more antimicrobial agents was observed in 46 (38.3%) E. coli isolates. The findings of STEC and MRD show that cattle carcasses may be a reservoir of pathogenic bacterial for the consumer public.
Key words: Multi-drug resistance, Escherichia coli, shiga toxin-producing Escherichia coli (STEC).
Lemos Sirlei Aparecida Maestá and Fernando Antonio de Ãvila, Renato Pariz Maluta, LÃvia Gerbasi Beraldo, Everlon Cid Rigobelo*, Manoel Victor Franco, Clarissa Araújo Borges
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-009, March, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and characterization of high caffeine-tolerant bacterium strains from the soil of tea garden
Fang-Yuan Fan1, Yan Xu1, Yue-Rong Liang1, Xin-Qiang Zheng2, Devajit Borthakur1,3 and Jian-Liang Lu1*
1Tea Research Institute, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
2Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Hangzhou 310058, China.
3Tocklai Experimental Station, Tea Research Association, Jorhat, Assam 785001, India.
Accepted 11 January, 2019
Abstract
Microbial biodegradation is an important and promising decaffeination approach because of its low cost and high security. The efficiency of this approach mainly depends on the characteristics of the strain. Twenty bacterium strains were isolated from soil of tea garden and exhibited high caffeine-tolerance, and these strains could grow on the medium supplemented with 20 g L-1 caffeine as the sole source for nitrogen and carbon. According to the physiological-biochemical characteristics and 16S rRNA gene sequence blast, two of them (CT25 and CT75) were identified as Pseudomonas putida. The results also showed that caffeine was not the preferential nutrition source for growth of strain CT25, and high level caffeine inhibited the bacterium amplification although caffeine could be metabolized by this strain. The CT25 grew well in agitated liquid medium when the incubation temperature was around 30°C. High initial concentration of strain inoculums would improve the efficiency of caffeine degradation.
Key words: Caffeine-tolerant bacterium, identification, 16S rRNA gene, caffeine biodegradation, growth condition.
Devajit Borthakur and Jian-Liang Lu*, Fang-Yuan Fan, Xin-Qiang Zheng, Yan Xu, Yue-Rong Liang