ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-007, January, 2018. © International Scholars Journals
Full Length Research Paper
Isolation and serotyping of Legionella pneumophila from Goreangab Dam and hostel shower heads in Namibia
Kazhila C. Chinsembu*, Hilaria Hakwenye and Jean-Damascène Uzabakiriho
Department of Biological Sciences, Faculty of Science, University of Namibia, P/Bag 13301, Windhoek, Namibia.
Accepted 23 September, 2017
Abstract
This is the first report of Legionella pneumophila serotypes in Namibia. Using modified buffered charcoal extract agar selective medium, L. pneumophila was isolated from Goreangab Dam water and University of Namibia (UNAM) student hostel shower heads. Latex agglutination tests showed that L. pneumophila serotypes 1 - 15 were present in Goreangab Dam water and serotypes 2 - 15 were present in blocks A and B of the UNAM old hostel shower heads. Further studies are needed to help understand L. pneumophila ecology and risk of human infection in Namibia.
Key words: Namibia, Legionella pneumophila, isolation, serotypes, dam water, shower heads.
Kazhila C. Chinsembu*, Hilaria Hakwenye and Jean-Damascène Uzabakiriho
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-006, January, 2018. © International Scholars Journals
Full Length Research Paper
Identification of the CagM's location in cytomembrane of Helicobacter pylori
Honglei Ding1, 2, 3, Tao Liu2, Linping Huang2, Weijun Zhang2, Ping Luo2, Qinghua Xie2, Zhen Liu2, Gang Guo2, Xuhu Mao2* and Quanming Zou1,2
1Institute of Sericulture and System Biology, Southwest University, Chongqing 400715, China.
2Department of Clinical Microbiology and Immunology, Faculty of Medical Laboratory Science, Third Military Medical University and National Engineering Technological Research Center of Immunological Biologicals, Chongqing 400038, China.
3Chongqing Productivity Council, Chongqing, China.
Accepted 19 October, 2017
Abstract
Helicobacter pylori infect nearly half of the world’s population and are associated with a spectrum of gastric maladies. The cytotoxin-associated gene pathogenicity island (cag PAI) encoding a type IV secretion system (T4SS) has been implicated in a series of host responses during infection. The cag PAI contains about 28 - 30 open-reading frames, for most of which the exact function is not well characterized or totally unknown and a delivered effector, CagA that becomes tyrosine phosphorylated upon delivery into host cells and initiates the changes in cell signaling. Here, we cloned one such cag PAI protein, CagM, which is encoded by the gene HP0537 from H. pylori strain 26695 and expressed the gene in Escherichia coli M15. 6xHis-tagged CagM protein was purified with one-step Ni-NTA affinity column chromatography. The IgG antibody against CagM was produced by immuning rabbit and purified by protein A sepharose chromatography, finally, we showed that CagM protein localized to the bacterial inner and outer membrane and the conclusion was consistent to some bioinformatics result.
Key words: Helicobacter pylori, CagM, type IV secretion system, expression, fractionation.
Linping Huang, Honglei Ding, Tao Liu, Xuhu Mao* and Quanming Zou, Gang Guo, Zhen Liu, Weijun Zhang, Ping Luo, Qinghua Xie
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-005, January, 2018. © International Scholars Journals
Full Length Research Paper
Prevalence and antimicrobial resistance patterns of methicillin-resistant staphylococci (MRS) isolated in a Veterinary Teaching Hospital in Brazil
Renato Pariz Maluta*, Ariel Eurides Stella, Everlon Cid Rigobelo, Ana Cláudia de Oliveira, Manoel Victor Franco Lemos and Fernando Antonio de Ávila
Programa de Pós-Graduação em Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil.
Accepted 4 August, 2017
Abstract
We investigated the prevalence of methicillin-resistant staphylococci (MRS) in humans and dogs and evaluated the antimicrobial resistance patterns of these bacteria at a Veterinary Teaching Hospital. Specimens from 50 human subjects and 50 dogs were studied. Isolates were identified by Gram-staining, biochemical reactivity and resistance to antimicrobials. While no isolates of methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-resistant Staphylococcus intermedius (MRSI) were isolated, two (4%) methicillin-resistant coagulase-negative staphylococci (MRCoNS) were isolated from dogs and 18 (36%) were isolated from humans. The percentage of MRCoNS isolates resistant to penicillin (100%), ciprofloxacin (30%), gentamicin (40%), clindamycin (25%), erythromycin (70%), trimethoprim-sulfamethoxazole (20%) or vancomycin (0%) was evaluated. The absence of MRS isolates resistant to vancomycin is of interest because this antimicrobial may be used as an important therapeutic alternative in cases of MRSA infections. Surveillance programs aimed against MRS should therefore be stimulated in veterinary health units.
Key words: Dogs, MRS, nosocomial, veterinary staff.
Renato Pariz Maluta*, Ariel Eurides Stella, Ana Cláudia de Oliveira, Manoel Victor Franco Lemos and Fernando Antonio de Ãvila, Everlon Cid Rigobelo
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-007, January, 2018. © International Scholars Journals
Full Length Research Paper
Antibacterial activity of propolis and honey against Staphylococcus aureus and Escherichia coli
M. Motior Rahman1,2*, Allan Richardson1 and M. Sofian-Azirun2
1Centennial College, Toronto, Canada.
2Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Accepted 6 August, 2017
Abstract
The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureus and Escherichia coli. The growth of S. aureus was inhibited by application of propolis and honey at concentrations of 2.74 to 5.48 mg ml-1 and 375. 0 at mg ml-1, respectively at both MIC and MBC. The greater inhibition zones (13.0 ± 0.09 to 15.0 ± 0.11 mm) were observed from propolis at concentrations of 2.74 to 5.48 mg ml-1 in the disc diffusion method which was closely correlated with the MIC, MBC and gradient-plate technique results. The combined results obtained from the disc diffusion test, MIC, MBC and gradient-plate techniques suggested that propolis at concentrations of 2.74 to 3.5 and 3.5 mg ml-1 was effective to inhibit S. aureus and E. coli, respectively. On the contrary, honey was effective to inhibit S. aureus at the concentration of 375.0 mg ml-1 but failed to inhibit E. coli growth at same concentration. The combined results from all methods indicated that both propolis and honey had antibacterial activity against S. aureus. Present findings also suggested that S. aureus is more susceptible to the effect of the propolis than its Gram-negative counterpart E. coli.
Key words: Antibacterial activity, honey, propolis, MIC, MBC, disc diffusion, Escherichia coli, Staphylococcus aureus.
Allan Richardson and M. Sofian-Azirun, M. Motior Rahman*
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-004, January, 2018. © International Scholars Journals
Full Length Research Paper
Diagnosis of infectious hematopoietic necrosis in rainbow trout hatcheries, Iran
Mehdi Raissy1*, Hasan Momtaz1, Mahsa Ansari2 and Manouchehr Moumeni3
1Faculty of Veterinary Medicine, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran.
2Young Researchers Club, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran.
3Fishery Research Center, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran.
Accepted 12 October, 2017
Abstract
Distribution of infectious hematopoietic necrosis (IHN) was studied in rainbow trout fish farms in Chaharmahal and Bakhtyari Province which is a major trout culture region in Iran. Overall 150 suspected rainbow trout fry with less than 3 g body weight from 30 fish farms in Chaharmahal and Bakhtyari Province, Iran, were studied and fishes of 10 hatcheries (33.3% of all studied farms) found to be infected with IHNV. Reverse transcription Polymerase Chain Reaction (RT-PCR) was applied to the detection of infectious hematopoietic necrosis virus (IHNV) using primer pairs. The product of 371 bp was amplified from the RNA extracts of infected organs of suspected fish. DNA sequencing of the PCR products demonstrated specificity of the amplification. The RT-PCR was found to be a simple, highly specific and sensitive method allowing diagnosis of IHN in a short time.
Key words: Reverse transcription-polymerase chain reaction (RT-PCR), IHN, rainbow trout.
Mahsa Ansari and Manouchehr Moumeni, Hasan Momtaz, Mehdi Raissy*
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (1), pp. 001-006, January, 2018. © International Scholars Journals
Full Length Research Paper
Production, purification and characterisation of extracellular L-asparaginase from a soil isolate of Bacillus sp.
Vidhya Moorthy1, Aishwarya Ramalingam1, Alagarsamy Sumantha1* and Rajesh Tippapur Shankaranaya2
1Department of Biotechnology, DKM College for Women (Autonomous), Sainathapuram, Vellore 632 001, India.
2Azyme Biosciences Private Limited, 1188/20, 3rd Floor, 26th Main, 9th Block, Jaya Nagar, Bengaluru 560 069, India.
Accepted 09 November, 2017
Abstract
L-asparaginase is an anti-neoplastic agent used in lymphoblastic leukaemia chemotherapy. Soil microbial isolates were screened for potential producers of L-asparaginase using a phenol red indicator growth medium and the microbe producing the largest hydrolysis zone was selected. The isolate was characterised by biochemical tests and was found to belong to Bacillus sp. The enzyme production was carried out by submerged fermentation. Two different carbon sources, glucose and maltose were used for the enzyme production and glucose was found to be the better carbon source. The enzyme was partially purified by ammonium sulphate precipitation. Dialysis was carried out to remove the excess salt and complete purification was achieved by ion -exchange chromatography. The characterised enzyme exhibited maximal enzyme activity at pH 7 and temperature 37°C. The enzyme was activated by MgCl2 and inhibited by EDTA. Protein profiling by SDS-PAGE revealed the molecular weight of the protein to be 45 kDa.
Key words: L-asparaginase, Bacillus, submerged fermentation, enzyme, purification.
Aishwarya Ramalingam, Vidhya Moorthy, Alagarsamy Sumantha* and Rajesh Tippapur Shankaranaya
Page: 1 - 6