ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Short Communication
Isolation and characterization of a Coffea canephora ERF-like cDNA
José Bustamante-Porras1, Michel Noirot2, Claudine Campa2, Serge Hamon2 and Alexandre de Kochko2*
1Instituto Nacional de Investigaciones Agrícolas (INIA), Bramón, Rubio, Táchira, Venezuela.
2Centre IRD, 911 Avenue Agropolis B.P. 5045, 34032 Montpellier Cedex 1, France.
Accepted 26 November, 2004
Abstract
ERFs (Ethylene-Responsive Element Binding Factors) are members of a transcription factors family unique to plants. They contain a well-conserved segment, which interacts specifically with sequences containing AGCCGCC motifs (GCC box) located in the promoter region of genes activated during biotic or environmental stress in plants. A cDNA corresponding to an ERF gene has been isolated from a Coffea canephora fruit cDNA library. The cDNA was 1,317 nucleotides long and has an open reading frame of 987 bp. The predicted polypeptide showed a great similitude with equivalent proteins from others plant species. The binding domain shows 98.3% identity in amino acids sequence with Lycopersicon esculentum ERF. This transcription factor may possibly be involved in differential cell growth or in fruit ripening process. Although it was not possible to isolate it from a leaf cDNA library, its presence in such library was confirmed.
Key words: Ethylene, coffee, Coffea canephora, transcription factor, ERF.
José Bustamante-Porras, Claudine Campa, Serge Hamon and Alexandre de Kochko*, Michel Noirot
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Isolation and characterization of micro satellites in Bambusa arundinacea and cross species amplification in other bamboos
Sumitra Nayak and Gyana Ranjan Rout*
Plant Biotechnology Division, Regional Plant Resource Center, Nayapalli, Bhubaneswar –751 015, Orissa, India
Accepted January 15, 2005
Abstract
Isolation and characterization of microsatellites was analyzed in Bambusa arundinacea and cross species amplification in other bamboos. Six microsatellites, three polymorphic and three monomorphic, were characterized in a bamboo species, Bambusa aruninacea belonging to the family Poaceae. The numbers of alleles per locus ranges form 2 to 6. Allelic diversity ranges from 0.128 to 0.789. Polymorphic Information Content (PIC) values for two loci were > 0.3, as an indicator of polymorphic allele. Cross species amplification has been tested in other 18 bamboo species. Monomorphic simple sequence repeats (SSRs) have been found to be cross amplified in most of the tested species while polymorphic ones in only three to four species. Utilization of the SSR loci in genetic diversity study of B. arundinacea and other cross amplified bamboo species is discussed.
Key words: Bambusa arundinacea, microsatellite marker, cross species amplification, population study, species identification.
Sumitra Nayak, Gyana Ranjan Rout*
Page: 1 - 10
Review
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Review
Crop protection strategies for major diseases of cocoa, coffee and cashew in Nigeria
T. O. Adejumo
Dept. of Microbiology, Adekunle Ajasin University, P.M.B. 01, Akungba-Akoko,Ondo State, Nigeria. E-mail: [email protected].
Accepted 19 December, 2004
Abstract
A great percentage of people in the developing countries are engaged in agriculture, but the yields of their produce are low due to diseases that plague their crops. In Nigeria, crop protection measures that are cheap, simple, cost-effective and sustainable are desirable to combat Phytophthora pod rot (black pod) and cocoa swollen shoot virus diseases of cocoa, coffee leaf rust and coffee berry diseases, inflorescence blight disease of cashew in order to make farming profitable and sustainable. Disease control strategies include the use of resistant cultivars, chemicals, biological, botanicals, cultural, physical controls and application of biotechnology, each of which is discussed in this paper.
Key words: Crop protection, cocoa, coffee, cashew, diseases.
T. O. Adejumo
Page: 1 - 10
Review
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Review
Application of biotechnology for the improvement of Nigerian indigenous leaf vegetables
Opabode, J.T*. and O.C. Adebooye
Department of Plant Science Obafemi Awolowo University Ile-Ife, NIGERIA
Accepted November 2004
Abstract
Nigeria is endowed with many indigenous leaf vegetables (ILVs) species, which spread across the estimated cultivable land area of 71.2 million hectares. These ILVs provide food, income, employment and herbal medicine to the population. Uncollected and uncharacterized germplasm, pests, diseases, anti-nutritional factors, recalcitrant seed, seed dormancy and perishable produce militate against the realization of potentials of the ILVs. This paper discusses biotechnological applications such as meristem culture, in vitro selection, zygotic embryo culture, somatic embryo genesis, protoplast culture, anther culture and genetic engineering that can solve improvement and production problems associated with some selected ILVs. Among the problems envisaged in the application of these biotechnological techniques are lacks of resources, selections of crop for research, attitude of government and weather conditions.
Key words: Indigenous leaf vegetables, biotechnology, production problems.
Opabode , J.T*. and O.C. Adebooye
Page: 1 - 10
Review
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Review
Antioxidant potential of African medicinal plants
S.E. Atawodi
Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria. E-mail: [email protected]; Tel: +234 8033 850 613.
Accepted 21 December, 2004
Abstract
Cellular damage or oxidative injury arising from free radicals or reactive oxygen species (ROS) now appears the fundamental mechanism underlying a number of human neurodegenerative disorders, diabetes, inflammation, viral infections, autoimmune pathologies and digestive system disorders. Free radical are generated through normal metabolism of drugs, environmental chemicals and other xenobiotics as well as endogenous chemicals, especially stress hormones (adrenalin and noradrenalin). Accumulated evidence suggests that ROS can be scavenged through chemoprevention utilizing natural antioxidant compounds present in foods and medicinal plants. Africa is blessed with enormous biodiversity resources, but plagued with several diseases, including those with ROS as the etiological factor. In this review, research on the antioxidant potential of medicinal plants of African origin between 1965 and September, 2004 is considered.
Keywords: Medicinal plant, antioxidant activity, chemoprevention, neurodegenerative diseases, Africa.
S.E. Atawodi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Microbial control of caged population of Zonocerus variegatus using Beauveria bassiana and Metarhizium sp.
O.E. FAGADE1, S.A. BALOGUN2 and C.J. LOMER3
1Environmental and Biotechnology Unit, Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.
2Environmental Microbiology Unit, Department of Microbiology, Adekunle Ajasin University, Akungba-Akoko, Nigeria.
3International Institute for Tropical Agriculture, Biological Control and Functional Biodiversity Centre, BP 08-0932 Cotonou, Benin Republic.
Accepted 13 October, 2004
Abstract
Microbial control of caged populations of Zonocerus variegatus was carried out using indigenous fungal entomopathogens isolated from the grasshopper’s cadaver. Bioassay response indicated a dose-dependent mortality coupled with drastic reduction in food consumption among spores infected grasshoppers. Lethal time (LT50) of 4.6 days for 103 spores/ml and 3.8 days for 107 spores/ml of oil formulation of Beauveria bassiana were observed. While Metarhizium sp recorded LT 50 of 9.0 days and 2.8 days for 103 and 107 spores/ml, respectively. The results obtained were discussed in relation to use of the isolates in the control Z. variegatus infestation in forest agro-ecosystem of south west, Nigeria.
Key words: Microbial control, entomopathogenic fungi, Beauveria bassiana, Zonoccrus variegatus, Metarhizium sp.
S.A. BALOGUN and C.J. LOMER, O.E. FAGADE
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