ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Rapid detection of Salmonella enterica in food of animal origins collected from Riyadh, King Saudi Arabia
Moussa I. M.1, Ashgan M. H.2, Mahmoud M. H.3 and Al-Doss A. A.4
1Center of Excellence in Biotechnology, King Saud University, P. O. 2460 Riyadh, King Saudi Arabia.
2College of Applied Studies and Community Service, King Saud University, King Saudi Arabia.
3Deanship of Scientific Research, King Saud University, Riyadh, King Saudi Arabia.
4College of Food and Agriculture Research, King Saud University, Riyadh, King Saudi Arabia.
Accepted 11 January, 2019
Abstract
The present study is aimed to investigate Salmonella species in food of animal origin collected from Riyadh, King Saudi Arabia (KSA) using conventional methods and polymerase chain reaction (PCR), targeting fimA gene specific for members of genus Salmonella. Salmonella isolation revealed 20 Salmonella serovars (8%) out of 250 examined samples. Nine strains (6, 92%) were recovered from 130 examined minced meats and 5 Salmonella strains (8.33%) were recovered from 60 local frozen chickens. Moreover, 6 Salmonella strains (10%) were isolated from 60 examined local chicken cuts. PCR using selective broth culture, Rappaport-Vassiliadis (RV) was used for the detection of different Salmonella species, targeting the fimA gene. All samples revealed positive results with bacteriological examination were positive by PCR -RV, and amplification of 120 bp fragments specific for fimA gene were observed, in addition, to 4 samples (1.6%) previously identified as negative samples with bacteriological examination were positive with PCR using the two primer pairs. The results revealed that the PCR-RV using primers specific for fimA gene could detect more positive samples of Salmonella species than conventional methods for rapid detection of food borne pathogens.
Key words: Salmonella species, fimA gene, polymerase chain reaction- Rappaport-Vassiliadis (PCR-RV), frozen chickens, minced meats.
Mahmoud M. H and Al-Doss A. A, Ashgan M. H, Moussa I. M
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-004, March, 2019. © International Scholars Journals
Full Length Research Paper
Molecular study for detection of Feline Leukemia Virus (FeLV) in Iranian cats
Farzad Shahrani1,2, Abbas Doosti1,2* and Asghar Arshi2
1Islamic Azad University, Shahrekord Branch, Young Researchers Club, Shahrekord, Iran.
2Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Accepted 21 January, 2019
Abstract
Feline leukemia virus (FeLV) is a retrovirus that infects cats. This infectious disease is a form of cancer of blood cells called lymphocytes. FeLV occurs in nature not as a single genomic species but as a family of closely related viruses. The aim of present study was to detect FeLV in Iranian domestic cats Reverse transcription polymerase chain reaction (RT-PCR). Fifty six blood samples were tested for FeLV using molecular techniques and out of 56 samples overall frequency of FeLV infection was 2.2%. The results showed that FeLV is a specific infection and the other common feline infectious pathogens and FeLV seem to be endemic in Iranian cats Vaccination and testing programs have proven to be effective in decreasing FeLV infection in Iran and may potentially totally eliminate it at least in other countries.
Key words: Feline leukemia virus, PCR, Iranian domestic cats.
Abbas Doosti* and Asghar Arshi, Farzad Shahrani
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-008, March, 2019. © International Scholars Journals
Full Length Research Paper
Response surface methodology for optimizing process parameters for the mass production of BEAUVERIA BASSIANA conidiospores
Priyanka Dhar and Gurvinder Kaur*
Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam, 781039, India.
Accepted 07 March, 2019
Abstract
BEAUVERIA BASSIANA is an insect pathogenic fungus and is currently being exploited as an effective commercial mycoinsecticide. Solid state fermentation is the most efficient way to mass produce B. BASSIANA conidiospores on solid substrates. Statistical optimization strategy was adopted to maximize the conidia production through solid state fermentation. Four substrates viz., rice (polished), crushed sorghum, wheat bran and rice bran at variable moisture content and yeast extract concentration were used. A full factorial central composite design was used to design the experiment and response surface method was used to study the optimal parameters required for large scale production. Optimization of the two most important factors like moisture content and yeast extract concentration at varied levels conferred best conidial yield of 28.8 × 109 /gm for the mixed substrate rice + wheat bran at 35% moisture content and 1.5% yeast extract concentration.
Key words: Beauveria bassiana, central composite design, conidiospores, response surface methodology, solid state fermentation.
Priyanka Dhar, Gurvinder Kaur*
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Rapid detection of Salmonella species using an improved gel-based DNA microarray method
Xuhong Ye1,2,3, Yiming Wang1,2 and Xiangui Lin1,2*
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Beijing East Road, 71 Nanjing 210008, PR China.
2Joint Open Laboratory of Soil and the Environment, Hongkong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China.
3Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
Accepted 10 January, 2019
Abstract
Salmonella, widely distributed in nature, is a great human and animal health hazard of a class of pathogens. Culture-based methods may require many days to detect Salmonella. Traditional microbiology could advantageously be replaced by DNA microarray technology. We described an improved 3-D polyacrylamide gel-based DNA microarray assay based on gyrB gene (DNA gyrase B subunit gene) sequences that can be used for the identification of Salmonella species. Primers specific for a gyrB gene region common to all 13 samples were synthesized and used for PCR amplification of purified DNA. An oligonucleotide probe for specific gyrB gene regions was developed for the identification of 7 Salmonella species. Acrylamide-modified oligonucleotides solutions containing acrylamide monomer, glycerol, APS and probe were prepared at the desired concentration. The solutions were spotted on the modified glass slide by ink jet using a microarrayer and then the slide was transferred to a vacuum chamber with TEMED, after that the slide was used for hybridization with fluorescently labeled ssDNA derived from amplified sample DNA to yield a pattern of positive spots. This microarray produced unique hybridization patterns for species of Salmonella and could differentiate closely related bacterial species. The sample preparation and microarray method used in this study increased sensitivity and reduces time-to-result for detection of Salmonella. The described method allowed microarray monitoring for Salmonella contamination of food and manure for aquaculture.
Key words: Salmonella, gyrB gene, PCR, DNA microarray, TEMED.
Yiming Wang and Xiangui Lin*, Xuhong Ye
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
Salt tolerance and mycorrhization of Bacopa monneiri grown under sodium chloride saline conditions
Khaliel A. S.1*, Shine K. 1 and Vijayakumar K.1,2
1Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
2Kerala State Council for Science, Technology and Environment, Thiruvananthapuram, Kerala, India.
Accepted 19 January, 2019
Abstract
Salinity of soil is a serious problem affecting plant growth and is increasing steadily in many parts of the world, particularly in arid and semi-arid areas. Arbuscular mycorrhiza (A M) is the most wide spread and significant mutualistic fungi having universal in their association including plants of agricultural and medicinal importance. A. mycorrhiza fungi have been shown to promote plant growth and salinity tolerance by various mechanisms. The effects of inoculation with two A. mycorrhizal fungi Glomus mosseae and Glomus intraradices have been investigated on B. monneiri, an important medicinal plant grown with five different levels of salinity (0.40, 80, 120 and 160 mM). Root colonization, leaf chlorophyll content and tolerance of the plants to salinity were determined. The results indicated that the A. mycorrhizal fungi could infect and colonize the roots effectively under high salinity levels and increased chlorophyll content. Dry mass production was significantly enhanced in the inoculated plants and the effect was more evident at the high salinity levels. More over, A. mycorrhizal colonization has increased Na+ and Cl- uptake and reduced rhizosphere NaCl level significantly. A. mycorrhizal association significantly increased tolerance of plants to salinity and was found as an effective measure to enhance establishment of the plant and to decrease soil salinity.
Key words: Arbuscular mycorrhiza, Glomus mosseae, Glomus intraradices, Bacopa monneiri, salt tolerance, proline.
Khaliel A. S*, Shine K. and Vijayakumar K
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-003, March, 2019. © International Scholars Journals
Full Length Research Paper
Seroprevalance of Coxiellosis in cows, sheep, goats and humans in Diyarbakir region of Turkey
Neval Berrin Arserim1, Simten Ye ilmen1, Osman Ya ar TEL2, Tuncer Özekinci3, Oktay Keskin2, Hüseyin Pulat4 and Aydin Vural5
1Dicle University, Faculty of Veterinary, Department of Microbiology, Diyarbakir, Turkey.
2Harran University, Faculty of Veterinary, Department of Microbiology, Sanliurfa, Turkey.
3Dicle University, Faculty of Medicine, Department of Microbiology, Diyarbakir, Turkey.
4Dicle University, Faculty of Veterinary, Department of Virolology, Diyarbakir, Turkey.
5Dicle University, Faculty of Veterinary, Department of Food Hygiene and Technology, Diyarbakir, Turkey.
Accepted 05 February, 2019
Abstract
This study aims at determining the seroprevalance of Coxiella burnetii in cows, sheep, goats and staff, working in the stock breeding sector in Diyarbakir region. Therefore, C. burnetii antibodies were investigated in sera samples of 612 sheep, 700 goats, 584 cows and 90 staff by enzyme-linked immunosorbent assays (ELISA). In the study, seropositivity was obtained as 25.4, 38.6, 20.0 and 6.6% in sheep, goats, cows and stockbreeding staff, respectively. Consequently, C. burnetii seropositivity, whether in people or in animals, had a ratio that should not be ignored in Diyarbakir region. Abort cases in ruminant should be assessed from the viewpoint of Coxiellosis. Also, people, especially those who are in risk group, should be made to be conscious of Coxiellosis infection, and measures for preventing this illness should be taken.
Key words: Cow, Coxiella burnetii, goat, human, seroprevalance, sheep.
Neval Berrin Arserim, Osman Ya ar TEL, Hüseyin Pulat and Aydin Vural, Simten Ye ilmen, Tuncer Özekinci, Oktay Keskin
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