ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Short Communication
Differentiation of parasitic and saprophytic leptospirae by oxidase test – A hasty modus operandi
Nagarajan Prabhu1*, Sivasamy Senthil Prabhu1, Mohammed Thanveer1, Nagarajan Revathi1, Narayanan Josna1, Rajaputhran Darsana 1, Danialas Joseph Pushpa Innocent2 and Palaniswamy Chinnaswamy3
1Postgraduate and Research Department of Microbiology, Arts and Science College, An Institution of Kovai Medical Center and Hospital, Coimbatore, India.
2Division of Microbiology, Rajah Muthiah Medical College and Hospital, Annamalai University, Chidambaram, India.
3Institute of laboratory Medicine, Kovai Medical Center and Hospital (KMCH), Coimbatore, India.
Accepted 14 October, 2012
Abstract
The use of the cytochrome oxidase test reagent, as a differential agent to study the pathogenic and saprophytic leptospires was studied. Growth of the leptospiral strains of the saprophytic group was almost involved in the conversion of colorless tetramethyl p-phenelene diamine dihydrochloride solution to dark brown color whereas pathogenic leptospirae strains showed reddish brown color. Saprophytic strains required 20-35 min for oxidation of cytochrome, however, pathogenic strains required more time, about 45 min for cytocrome oxidation. By this preliminary investigation, it is now possible to differentiate and report the saprophytic and parasitic strains based on the differential observation of the cytochrome oxidase test.
Key words: Pathogenic, leptopsiral strains, saprophytic strain, 1% oxidase reagent, rapid detection.
Narayanan Josna, Nagarajan Revathi, Mohammed Thanveer, Danialas Joseph Pushpa Innocent and Palaniswamy Chinnaswamy, Sivasamy Senthil Prabhu, Rajaputhran Darsana, Nagarajan Prabhu*
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Research Article
Qasem A. Jafar*, Al-Zinki Sameer, Al-Marzouk Ahmed and Al -Sharifi Faisal, Al-Amad Samee, Al-Mouqati Salwa
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Review
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Review
Multidrug resistance in Pseudomonas aeruginosa: Insights into the molecular mechanisms
Sriram Seshadri* and Chintan Chhatbar
Department of Biochemistry and Biotechnology, Institute of Science, Nirma university of Science and Technology, Ahmedabad, S G Highway, Ahmedabad - 321481, Gujarat, India.
Accepted 4 May, 2013
Abstract
Pseudomonas aeruginosa is commonly found in chronic lung infections such as cystic fibrosis and others. Intrinsic antibiotic resistance of P. aeruginosa accompanied by its ability to acquire resistance via mutations and adapt to the heterogeneous and dynamic environment of chronically infected lungs are major threats and reasons for the ultimate failure of the current antibiotic therapies in eradicating the infection from lungs. New insights at molecular levels in the process of accumulating such beneficial mutations at faster rates, termed as hypermutation have allowed us to understand the high acquired resistance of this opportunistic pathogen. Also, these understandings will allow us to develop new therapeutic strategies to combat chronic infections.
Key words: Pseudomonas aeruginosa, chronic lung infections, multi drug resistance, hypermutation.
Sriram Seshadri*, Chintan Chhatbar
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Research Article
Ofodile A. C., Abubakar A., Junaid S. A.*, Olabode A. O., Okwori A. E. J. and Adetunji J. A., Echeonwu G. O. N.
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Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Screening for bacteria agents responsible for otitis media and their antibiogram
Oyeleke, S. B.
Department of Microbiology, Federal Universities of Technology, Minna Niger State. E-mail: [email protected].
Accepted 08 December, 2012
Abstract
30 samples (male and female) of ear swab were collected from patients at general hospital Minna for examination of bacteria that causes otitis media. 26 samples were positive for these organisms. The organisms isolated include Pseudomonas sp., Kleblesella sp., Proteus sp., Escherichia coli and Staphylococcus aureus. Pseudomonas sp., (54%) had the highest incidence of occurrence followed by Kleblesella sp. (19%) and Proteus sp (15%) while the least was S. aureus (4%). The organisms’ sensitivity test reveals that they were highly sensitive to gentamycin. The bacteria responsible for otitis media are pathogenic, therefore effort should be made to reduce the factors militating its occurrence of these pathogens in the community.
Key words: Otitis media, eustachian tube, salpingitis, gentamicin, prevalence, pathogenic.
Oyeleke , S. B.
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