ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-005, February, 2018. © International Scholars Journals
Full Length Research Paper
Bacillus pumilus, a new pathogen on potato tubers in storage in Mali
Hamed Bathily2, Amadou Hamadoun Babana1* and Fassé Samaké1
1Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Bamako, Bamako, Mali BP E3206.
2Laboratory of soils Microbiology, Faculty of Science and Technology, University of Bamako, Bamako, Mali BP E3206.
Accepted 12 December, 2017
Abstract
Soft rot occurred severely in potato tubers stored in traditional and ameliored storehouses at Sikasso, Mali. 17 infective bacterial isolates were isolated from potato rot tissues (Solanum tuberosum L var. Odessa). Out of all, the isolate Od23 was found pathogenic and was characterized as rod-shaped, Gram positive, endo-spore formers and yellow pigment producers. This isolate which was found to be the principal organism responsible for potato rot in storage in Sikasso, grew at a temperature range from 5 to 45°C, with optimum temperature of 30 - 35°C. However, it showed strong pathogenicity to potato tubers at 30°C at 3 days. Furthermore, the 16S DNA analysis confirmed that the obtained isolate was Bacillus pumilus. All Potato varieties cultivated in Mali responded to infection with B. pumilus. Potato var. Sahel was the most susceptible, while Pamina appeared the most resistant potato variety from Mali. According to literature review, this is the first report on the occurrence of B. pumilus as a causal agent of potato soft rot in storage in the region of Sikasso, Mali.
Key words: Potato, storage, Bacillus pumilus, soft rot, Mali.
Hamed Bathily, Amadou Hamadoun Babana* and Fassé Samaké
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-012, February, 2018. © International Scholars Journals
Full Length Research Paper
Assessment of the temporal change in groundwater quality when stored at different temperatures in household conditions, in the equatorial region of Central Africa
Moïse Nola1*, Ernest Djarmaila1, Norbert Kemka1,2, Serge H. Zébazé Togouet1, Nour-Eddine Chihib3, François Krier3, Pierre Servais4, Jean-Pierre Hornez3 and Thomas Njiné1
1Laboratory of General Biology, Faculty of Sciences, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon.
2Hydrology Research Centre, Mining and Geological Research Institute, P. O. Box 4110 Nlongkak, Yaounde, Cameroon.
3Département de Génie Université Biologique, des Sciences et Technologies de Lille 1, IUT « A », Laboratoire ProBioGEM, Bd Paul Langevin – Cité Scientifique, B. P. 179 – 59 653 Villeneuve d’Ascq cedex France.
4Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Campus de la Plaine CP 221, Boulevard du Triomphe, 1050 Bruxelles, Belgique.
Accepted 04 December, 2018
Abstract
The study carried out aimed at assessing the impact of groundwater storage temperature at household conditions on the temporal evolution of electrical conductivity and the future of heterotrophic aerobe bacteria (HAB). The storage duration was 7 days and the considered temperatures were 3, 10, 18 and 25°C. The electrical conductivity during storage reached 829 µS/cm at 3 - 18°C and 850 µS/cm at 25°C. The maximum HAB abundance was 9 x 103 cfu/ml at 3°C, 41 x 103 cfu/ml at 10°C, 44 x 103 cfu/ml at 18°C and 93 x 103 cfu/ml at 25°C. At the 3rd and 7th days storage at 3°C, changes in bacterial abundances values were significantly in the same direction as those of electrical conductivity (P < 0.05). The highest cell apparent growth rate at the 3rd day storage was 0.249 d-1 at 3°C, 0.559 d-1 at 10°C, 0.924 d-1 at 18°C and 1.233 d-1 at 25°C. However, at the 7th day storage, it was 0.362 d-1 at 3°C, 0.497 d-1 at 10°C, 0.690 d-1 at 18°C and 0.672 d-1 at 25°C. At the 3rd day storage, a decrease in cell abundance was noted in 90% of samples at 3°C and the cell apparent inhibitory rate varied from 0.012 to 0.989 d-1. The storage of groundwater in households’ conditions for a long period would alter its bacteriological quality.
Key words: Bacterial abundance’s evolution, electrical conductivity, groundwater, storage temperature, duration.
Norbert Kemka, Nour-Eddine Chihib, François Krier, Moïse Nola*, Pierre Servais, Ernest Djarmaila, Jean-Pierre Hornez and Thomas Njiné, Serge H. Zébazé Togouet
Page: 1 - 12
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-007, February, 2018. © International Scholars Journals
Full Length Research Paper
Inhibitory activity of Paenibacillus macerans and Paenibacillus polymyxa against Ralstonia solanacearum
Bin Li1*, Ting Su1, Rongrong Yu2, Zhongyun Tao1, Zhiyi Wu3, Soad A. E. Algam1, Guanlin Xie1, Yanli Wang4 and Guochang Sun4
1State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
2School of Education Science and Technology, Zhejiang University of Technology, Hangzhou 310032, China.
3Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310012, China.
4Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Accepted 11 November, 2017
Abstract
The inhibitory activities of seven Paenibacillus polymyxa strains and nine Paenibacillus macerans strains against Ralstonia solanacearum strains were examined. Result from this study indicated that the growth of all R. solanacearum strains except strain E406 were inhibited by P. macerans MB02-992 and P. polymyxa MB02-1007, while the other fourteen Paenibacillus strains had no in vitro inhibitory effect against R. solanacearum strains. In addition, suspensions of the two antagonistic bacteria showed antibacterial activities against R. solanacearum under different treatments and reduced the disease incidence and severity of tomato bacterial wilt. Overall, this study clearly demonstrates that antagonistic substances may play an important role in biocontrol of the two antagonistic bacteria. However, antimicrobial activities of P. macerans and P. polymyxa depend on the Paenibacillus strains and the target pathogen. This is the first report about the antibacterial activities of Paenibacillus strains against R. solanacearum strains isolated from different host plants.
Key words: Paenibacillus, antagonistism, biovar, Ralstonia solanacearum, tomato wilt.
Yanli Wang and Guochang Sun, Zhongyun Tao, Guanlin Xie, Soad A. E. Algam, Zhiyi Wu, Bin Li*, Ting Su, Rongrong Yu
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-005, February, 2018. © International Scholars Journals
Full Length Research Paper
A microbiologic evaluation of patients with chronic prostatitis and relation with clinical symptoms
Fahriye Eksi1*, Ahmet Erbagci2, Sakip Erturhan2, Iclal Balci1, Mehmet Solakhan2 and Faruk Yagci2
1Gaziantep University, Faculty of Medicine, Department of Medical Microbiology, Gaziantep, Turkey.
2Gaziantep University, Faculty of Medicine, Department of Urology, Gaziantep, Turkey.
Accepted 13 July, 2017
Abstract
In this study urine and prostatic secretion samples of patients with chronic prostatitis were investigated for various prostatitis pathogens and the correlation between clinical symptoms. A total of 60 patients with chronic prostatitis were examined, prostatic secretion and urine specimens were evaluated under direct microscopy after staining with Giemsa and Gram. The selective media were used to investigate the presence of bacterial pathogens, Mycoplasma hominis and Ureaplasma urealyticum. Based on the laboratory findings of patients, 11(18.3%) were found to have chronic bacterial prostatitis, 49 (81.7%) were found to have chronic pelvic pain syndrome. Organisms isolated in patients with chronic bacterial prostatitis included Escherichia coli in four cases, Staphylococcus aureus in two cases and Pseudomonas aeruginosa, Klebsiella pneumoniae, coagulase- negative staphylococci, Enterococcus faecalis and diphtheroids in one case each. Of the 49 patients with chronic pelvic pain syndrome, U. urealyticum was isolated in seven and M. hominis in one of the patients. Premature ejaculation was more frequently detected in the patient group with chronic bacterial prostatitis than the patient group with chronic pelvic pain syndrome. Analysis of etiology of chronic prostatitis in our patients showed that U.urealyticum and E. coli were common pathogens of chronic prostatitis. We concluded that chronic bacterial prostatitis may increase the risk of premature ejaculation.
Key words: Chronic prostatitis, etiology, Ureaplasm urealyticum, Mycoplasma hominis, premature ejaculation.
Mehmet Solakhan and Faruk Yagci, Sakip Erturhan, Fahriye Eksi*, Ahmet Erbagci, Iclal Balci
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-009, February, 2018. © International Scholars Journals
Full Length Research Paper
Antioxidant and antimicrobial activities of Turkish endemic Achillea species
Ismail Turkoglu1, Semra Turkoglu2*, Sait Celik2 and Mustafa Kahyaoglu3
1Department of Biology Education, Education Faculty, Fırat University, Elazı , Turkey.
2Department of Chemistry, Faculty of Science and Arts, Fırat University, Elazı , Turkey.
3Department of Primary Education, Faculty of Education, Siirt University, Siirt, Turkey.
Accepted 23 July, 2017
Abstract
This study was undertaken to examine in vitro antioxidant, radical scavenging and antimicrobial activities of extracts of Achillea schischkinii Sosn. and Achillea teretifolia Waldst. and Kitt (Asteraceae). The plant materials were extracted in methanol, water and chloroform using rotary evaporator apparatus. The extracts were screened for antioxidant activity using the ABTS radical scavenging capacity, DPPH radical scavenging capacity, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities and compared to standard antioxidants. The results obtained in this study indicate that A. schischkinii and A. teretifolia are potential sources of natural antioxidants, antimicrobial activity screening was performed by the disc diffusion method against 6 bacteria strains and 2 yeast species. A. teretifolia displayed strong inhibitory effects against Pseudomonas aeruginosa. The A. teretifolia extract also showed antimicrobial activity against Saccharomyces cerevisae. I contrast, the A. schischkinii extract showed no antimicrobial activity against gram-positive and gram-negative bacteria nor against yeast.
Key words: Achillea schischkinii, Achillea teretifolia, antioxidant, antimicrobial.
Sait Celik and Mustafa Kahyaoglu, Semra Turkoglu*, Ismail Turkoglu
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-005, February, 2018. © International Scholars Journals
Full Length Research Paper
Detection of deep fungal infection in SLE patients with multiplex fluorescent real-time PCR
Ledong Sun, Caimei Zhong, Mingyu Chen, Bing Wang, Xueyan Liu, Xiufen Zheng, Fengjiao He, Zaigao Zhou and Kang Zeng*
Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Accepted 13 August, 2017
Abstract
To establish a rapid, sensitive and specific detection method for deep fungal infection, we detected the deep infection of Candida albicans and Aspergillus flavus in patients with systemic lupus erythematosus by using multiplex fluorescent real-time PCR (MFRT-PCR). Firstly, the positive rate, sensitivity and specificity of multiplex fluorescent quantitative PCR for detecting the fungal infection was tested in 20 specimens from systemic lupus erythematosus patients with C. albicans and A. flavus infections. Then, 20 specimens from SLE patients with suspected deep fungal infections and 20 other microorganism infections were detected with MFRT-PCR. Results showed the positivity rate and specificity of both 100% for detecting the deep C. albicans and A. flavus infection. Its detecting sensitivity for deep fungal infection was 75%, which was significantly higher than that of fugal culture method (40%) (P < 0.05). MFRT-PCR is a better method for detecting deep C. albicans and A. flavus infections, with higher sensitivity and specificity than fungal culture.
Key words: Systemic lupus erythematosus, deep fungal infection, multiplex fluorescent quantitative PCR.
Ledong Sun, Bing Wang, Zaigao Zhou and Kang Zeng*, Fengjiao He, Caimei Zhong, Xueyan Liu, Xiufen Zheng, Mingyu Chen
Page: 1 - 5