ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-005, May, 2019. © International Scholars Journals
Full Length Research Paper
In vitro assessment of Inula spp. organic extracts for their antifungal activity against some pathogenic and antagonistic fungi
Faten Omezzine1*, Mejda Daami-Remadi3, Asma Rinez1, Afef Ladhari1 and Rabiaa Haouala2
1Department of Biology, Faculty of Sciences of Bizerte, University of Carthage, Amilcar 1054, Tunisia.
2Department of Biological Sciences and Plant Protection, Higher Agronomic Institute of Chott Meriem,
University of Sousse, Chott Meriem 4042, Tunisia.
3Laboratory of Plant Pathology, Regional Center of Research in Horticulture and Organic Agriculture, University of Sousse, Chott Meriem 4042, Tunisia.
Accepted 16 April, 2019
Abstract
Inula viscosa, Inula graveolens and Inula crithmoïdes (Asteraceae) leaf and flower organic extracts (hexane, chloroform and methanol) were assessed for their antifungal activity against two Trichoderma species (Trichoderma harzianum and Trichoderma viride) and three formae speciales of Fusarium oxysporum. I. viscosa organic extracts showed an important inhibitory activity against all target fungal isolates. Growth inhibition percentage ranged between 17-61, 77-100, and 55-100% in presence of hexane, chloroform and methanol I. viscosa leaf extracts, respectively. Flower organic extracts reduced mycelial growth of all fungi by 32-66, 30-75, and 8-70%, respectively. For I. graveolens, Stem + leaf organic extracts showed more or less important inhibition depending on solvent nature, though, flower organic extracts were found to be the most effective against tested fungi: a total inhibition of growth was recorded with methanol fraction against all target fungi and with hexane fraction against F. oxysporum f. sp. melonis (FOM) and T. viride. In presence of I. crithmoïdes leaf organic extracts, a total growth inhibition was noted with the three extracts against T. harzianum, with hexane and methanol fraction against T. viride and with chloroform fraction against FOM. I. crithmoïdes flower extracts caused a highly significant growth inhibition of about 57-100, 66-100, and 100-100% with hexane, chloroform and methanol extracts, respectively. Therefore, I. viscosa, I. graveolens and I. crithmoïdes could be an important source of biologically active compounds useful for developing environmentally safe antifungal products.
Key words: Antifungal activity, organic extracts, Inula, radial growth.
Asma Rinez, Afef Ladhari and Rabiaa Haouala, Faten Omezzine*, Mejda Daami-Remadi
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-006, May, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and characterization of a biosurfactant-producing bacterium from Daqing oil-contaminated sites
Tong Liu1, Jumei Hou1, Yuhu Zuo1*, Sining Bi1 and Jing Jing2
1Institute of Plant Pathology and Applied Microbiology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P. R. China.
2School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, P. R. China.
Accepted 09 April, 2019
Abstract
Biosurfactants are biodegradable, non-toxic and ecofriendly compounds released by microorganisms. Most of the microorganisms were isolated from contaminated sites by petroleum hydrocarbon and industrial wastes. In this study, a biosurfactant-producing strain was isolated from Daqing oil-contaminated sites in China by enrichment culture, hemolytic activity assay, hydrolyzing oil activity assay, improved degreasing effect assay, and emulsification activity measurement. The strain was identified as Pseudomonas sp. BS1 based on its physiological characteristics and analysis of its 16S rRNA gene sequence. The identified isolate is capable of producing glycolipids or other anionic surfactants as determined in our analysis of a phenotypic assay using CTAB. The biosurfactant was isolated from the culture supernatant and identified as dirhamnolipids (Rha-Rha-C10-C10) by high-performance liquid chromatography–mass spectrometry. The culture filtrate and cell-free supernatant produced by this isolate were found to be highly effective in oil displacement from oil sand, suggesting that the isolate has potential use in enhanced oil recovery.
Key words: Biosurfactant, Pseudomonas sp., dirhamnolipids, microbial enhanced oil recovery.
Yuhu Zuo*, Sining Bi and Jing Jing, Tong Liu, Jumei Hou
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-005, May, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and characterization of EST-SSRs in the Ramie
Jian-Hua Chen1, Ming-Bao Luan1, Shu-Feng Song 2, Zi-Zheng Zou1, Xiao-Fei Wang1, Ying Xu1, and Zhi-Min Sun1
1Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key laboratory of Stem-fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha China.
2Institute of subtropical agricultural Chinese academy of science, Changsha, China.
Accepted 22 March, 2019
Abstract
In order to develop the more EST-SSRs, 320 ESTs of ramie from NCBI were analyzed. 76 SSR primer-pairs developed from 320 ESTs were tested among 62 ramie individuals, and 27 SSR loci were polymorphic. Among the 27 primers, the numbers of alleles per locus ranged from two to five, in which 19 primers amplify two alleles, seven primers amplify three alleles, and only one primer amplifies five alleles. Observed heterozygosity (HO) of overall loci among the 27 primer pairs ranged from 0.16 to 0.93 and expected heterozygosity(HE) ranged from 0.21 to 0.66.Of the 27 loci, 14 displayed significant deviations from Hardy–Weinberg expectations (P < 0.05). No significant linkage disequilibrium (LD) was detected among the 27 loci. The 27 primers have been used in the ramie molecular linkage map and genetic diversity analysis successfully.
Key words: Microsatellite marker, ramie, EST, EST-SSR.
Zi-Zheng Zou, Xiao-Fei Wang, Shu-Feng Song, Ming-Bao Luan, and Zhi-Min Sun, Ying Xu, Jian-Hua Chen
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-004, May, 2019. © International Scholars Journals
Full Length Research Paper
Bacteremia due to multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing Acinetobacter baumannii
Abdollah Bazargani and Zahra Hashemizadeh*
Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Accepted 22 March, 2019
Abstract
Bacteremia due to Acinetobacter baumannii is a common problem in hospitals worldwide. Physicians in the intensive care units (ICUs) encountered a serious challenge of finding a drug to cure the extended-spectrum beta-lactamase (ESBL) producing and multi-drug resistant (MDR) A. baumannii bacteremia. Our aim was to determine the frequency of A. baumannii bacteremia in an Iranian hospital ICUs, their antibiotic susceptibility patterns, and the frequency of ESBLs by a cross-sectional study. A total of 340 patients admitted to ICUs during a 6 month period of study were investigated for bacteremia due to A. baumannii. Bacteria isolates from blood specimens were identified as A. baumannii by API 20NE system. Antimicrobial susceptibility was studied with disk diffusion method. Detection of ESBLs was done by double disk synergy test. Of the 340 patients investigated, bacteremia was found in 114 cases (33%). A. baumannii was diagnosed as the etiological agent of bacteremia in 69 cases (60.5%). All the isolates were multi-drug resistant. Except one, all the remaining isolates (98.6%) were resistant to at least 7 of 13 tested antibiotics. Pandrug-resistance was observed in 4 isolates (5.6%). Of all the isolated A. baumannii, 49 (71%) were found to be resistant to cephalosporins by screening tests and among them 27 isolates (39%) were found to be ESBL producing. Our study showed a high frequency of A. baumannii bacteremia occurrence in our hospital ICUs. An urgent intervention is needed to reduce the MDR bacterial load in these critical units.
Key words: Acinetobacter baumannii, bacteremia, multi-drug resistant, extended-spectrum beta-lactamase.
Zahra Hashemizadeh*, Abdollah Bazargani
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-004, May, 2019. © International Scholars Journals
Full Length Research Paper
Identification of some Penicillium species by traditional approach of morphological observation and culture
Suhaib A. Bandh1*, Azra N. Kamili1 and Bashir A. Ganai2
1Microbiology and Pathology Laboratory, Centre of Research for Development and Department of Environmental Science, University of Kashmir, Srinagar-190006, India.
2Department of Biochemistry, University of Kashmir, Srinagar-190006, India.
Accepted 16 March, 2019
Abstract
Ninety six samples of water collected from Dal Lake, Kashmir at eight different sites for a period of one year from April 2010 to March 2011 were tested for the presence of Penicillium species. Five different species of the said genus were isolated from the samples by serial dilution technique. Out of total 122 fungal colonies isolated, five species of Penicillium viz, Penicillium olivicolor, Penicillium commune, Penicillium chrysogenum, Penicillium funiculosum and Penicillium dimorphosporum were obtained. Among the isolated species P. funiculosum was the most abundant (28.7%) followed by P. chrysogenum (27.04%), P. dimorphosporum (23.77%), P. olivicolor (16.40%) and P. commune (4.09%). Maximum percentage of fungal colonies (27.7%) was observed at site PKB followed by TBN (21.13%), DLG and GB (13.15%) each, NL (8.45%), BHN (7.04%), HB (5.63%) and BD (3.76%).
Key words: Macroscopic, microscopic, Dal Lake, serial dilution, water, Penicillium.
Azra N. Kamili and Bashir A. Ganai, Suhaib A. Bandh*
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-008, May, 2019. © International Scholars Journals
Full Length Research Paper
Molecular cloning and extracellular expression of cyclodextrin glycosyltransferase gene from Bacillus sp. NR5 UPM
Norhayati Ramli1, Suraini Abd-Aziz1*, Mohd Ali Hassan1, Noorjahan Banu Alitheen2, Kamarulzaman Kamaruddin3 and Zoolhilmi Ibrahim3
1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
3Environment and Bioprocess Technology Centre, SIRIM Berhad, No. 1, Persiaran Dato’ Menteri, Section 2, 40911 Shah Alam, Malaysia.
Accepted 16 March, 2019
Abstract
The cloning of a polymerase chain reaction (PCR) gene fragment from Bacillus sp. NR5 UPM isolated from the soil in Malaysia into an Escherichia coli expression vector was successfully carried out. Analysis of the nucleotide sequences revealed the presence of an open reading frame of 2112 bp which encoded a protein containing 704 amino acids with a putative molecular weight of 78.6 kDa. The deduced amino acids sequence showed about 98% homology with the CGTase from Bacillus sp. KC201. Compared to the wild type, the CGTase that was produced in E. coli cells only required one-fourth of culture time and neutral pH to produce CGTase. After 12 h of cultivation, the CGTase activity in the culture medium reached 29.6 U/ml, which was approximately 2.5-fold higher than the CGTase from the parental strain. The CGTase was produced extracellularly by E. coli (94%) indicating the signal peptide was functional in E. coli.
Key words: Molecular cloning, nucleotide sequence, cyclodextrin glycosyltransferase, Bacillus sp. NR5 UPM.
Suraini Abd-Aziz*, Norhayati Ramli, Noorjahan Banu Alitheen, Kamarulzaman Kamaruddin and Zoolhilmi Ibrahim, Mohd Ali Hassan
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