ISSN 2736-1756
Research Article
Ademola O. Olaniran* and Balakrishna Pillay, Simphiwe P. Buthelezi
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Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756, Vol. 17 (3), pp. 001-004, March, 2023. © International Scholars Journals
Full Length Research Paper
Characterization and Detection of the Scuticociliate Philasterides dicentrarchi in Indo-Pacific Seahorses Hippocampus kuda
Sang Phil Shin1, Jee Eun Han1, Dennis K. Gomez2, Ji Hyung Kim1, Casiano H. Choresca Jr.1, Jin Woo Jun1 and Se Chang Park1*
1Laboratory of Aquatic Animal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151 to 742, Republic of Korea.
2Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines in the Visayas, Miagao, Iloilo 5023, Republic of the Philippines.
Accepted 17 February, 2023
Abstract
The “indo-pacific seahorses”, Hippocampus kuda which had been reared in one of the private commercial aquaria for exhibition were sent to the laboratory for diagnosis. Feces and intestinal contents from 5 seahorses were examined microscopically and revealed numerous scuticociliates. We identified the species of scuticociliate by light (wet mount) and scanning electron microscopy, PCR (polymerase chain reaction) and sequencing. This paper reports the first identification of scuticociliate Philasterides dicentrarchi from seahorse in Korea.
Key words: Indo-pacific seahorse, Philasterides dicentrarchi, scanning electron microscopy, polymerase chain reaction, sequencing.
Sang Phil Shin, Jin Woo Jun and Se Chang Park*, Jee Eun Han, Dennis K. Gomez, Casiano H. Choresca Jr., Ji Hyung Kim
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Research Article
Hossein Khavari-Daneshvar and Sara Jamali, Monireh Rahimkhani*
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Research Article
Sultan Ayesh Mohammed Saghir, Anwar Kassem Al-Madhagi and Khaled Abdulkarim Al-Moyed, Abdulrahman Ali Mohammed Zabad*
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Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756, Vol. 17 (1), pp. 001-007, January, 2023. © International Scholars Journals
Full Length Research Paper
Molecular Characterization of CTX-M ESBLs among Pathogenic Enterobacteriaceae isolated from different regions in Sudan
Hisham N Altayb1*, Mohamed A M Siddig2, Nagwa M El Amin3, Ahmed Ibrahim Hashim1 Maowia M. Mukhtar4
1College of medical laboratory science, Sudan University of science and technology, Sudan.
2Botany department, Faculty of Science, University of Khartoum, Sudan.
3Department of microbiology, Faculty of medicine, university of Khartoum, Sudan.
4Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
Accepted 07 November, 2022
Abstract
This was a cross sectional study conducted to detect and characterize CTX-M genes among extended spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolated from different regions in Sudan. A total of 305 of Enterobacteriaceae clinical isolates were collected from different regions in Sudan. ESBLs production was initially screened by cefotaxime, cefepime and Ceftazidime, then confirmed by disk combination method and PCR. DNA sequencing was done to differentiate betwen blaCTX-M genotypes. Escherichia coli was the most predominant isolate (58%), followed by Klebsiella pneumoniae (26.6%), Citrobacter freundii (3.6%), Enterobacter species (6.2%) and Proteus species (5.6%). ESBLs were detected by disk combination method in 128/305 (42%) of the tested isolates; Khartoum State 23/36 (64%), Gizera State 54/100 (54%), Sinnar State 49/92 (53%) and White Nile State 2/77 (2.6%). Three quarters of the ESBLs producers (96/128) were positive for blaCTX-M genes by PCR. The blaCTX-M-15 gene was the most predominant gene 18/23 (78.3%), followed by blaCTX-M-14 3/23(13%), blaCTX-M-27 1/23 (4.3%) and blaCTX-M-98 1/23 (4.3%). There was a transition mutation (substitution of A with G at position 25) in the blaCTX-M gene (ID: KP309815), that affected protein structure. In conclusion blaCTX-M-15 was the most commonly encountered gene and widely spread in different Sudanese regions.
Keywords: blaCTX-M; Beta-Lactamases Genes; Enterobacteriaceae; Sudan
Ahmed Ibrahim Hashim and Maowia M. Mukhtar, Mohamed AM Siddig, Nagwa M El Amin, Hisham N Altayb*
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Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756, Vol. 16 (6), pp. 001-005, June, 2022. © International Scholars Journals
Full Length Research Paper
Investigatıon of Epstein-Barr virus serology and DNA in bone marrow transplant recipients
Inci M.1*, Gokahmetoglu S.2, Kaynar L.3, Ercal B. D.2, Durmaz S.2 and Buyukoglan R.3
1Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey.
2Erciyes University, School of Medicine, Department of Medical Microbiology, Kayseri, Turkey.
3Erciyes University, School of Medicine, Department of Hematology-Oncology, Kayseri, Turkey.
Accepted 25 April, 2022
Abstract
The aim of this study was to investigate Epstein-Barr virus (EBV) serology and EBV DNA in the patients with bone marrow transplantation. In the current study, 128 patients with bone marrow transplantation (BMT) were included in Hematology-Oncology Department of Medical Faculty, Erciyes University between June 2005 and October 2009. In order to evaluate EBV serology and DNA, 357 samples obtained from the patients were studied with enzyme-linked immunosorbent assay and real-time polymerase chain reaction (PCR). The components of EBV serology were anti-Ebstein-Barr nuclear antigen (EBNA)-1 IgG, anti- viral capsid antigen (VCA) IgG and IgM. EBV seropositivity was found in 98.4% of the samples. EBV DNA positivity was detected in 13.3% of the patients. High viral load was only seen in one patient among all the EBV DNA positivity. No patient showed any symptoms regarding EBV virus and admitted with post-transplant lymphoproliferative disease. In conclusion, for the diagnosis and follow up of EBV infections in patients with BMT, detection of EBV DNA by PCR method is also beneficial in addition to serological tests regarding EBV.
Key words: Epstein-Barr virus, serological markers, real-time PCR, bone marrow transplant recipients.
Ercal B. D, Gokahmetoglu S, Inci M*, Durmaz S and Buyukoglan R, Kaynar L
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