ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Amplification of 1-amino- cyclopropane-1-carboxylic (ACC) deaminase from plant growth promoting rhizobacteria in Striga-infested soil
Olubukola O. Babalola1,2*, Ellie O. Osir2 , Abiodun I. Sanni1, George D. Odhiambo3, and Wallace D. Bulimo2,Ψ
1Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria
2International Centre of Insect Physiology and Ecology, Nairobi, Kenya
3Kenya Sugar Research Foundation, Kisumu, Kenya.
Accepted 28 May 2003
Abstract
Experiments were conducted in pots to determine the growth effect of different rhizobacteria on maize under Striga hermonthica infestation. Three bacteria were selected based on their plant growth promoting effects. Whole bacterial cells of the rhizobacteria were used to amplify 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase gene by polymerase chain reaction (PCR). Each bacterial inoculation increased agronomic characteristics of maize although not always to a statistically significant extent. The extent of growth enhancement differs between the isolates. Enterobacter sakazakii 8MR5 had the ability to stimulate plant growth, however in the PCR study, ACC deaminase was not amplified from this isolate, indicating that not all plant growth-promoting rhizobacteria contain the enzyme ACC deaminase. In contrast, an ACC deaminase specific product was amplified from Pseudomonas sp. 4MKS8 and Klebsiella oxytoca 10MKR7. This is the first report of ACC deaminase in K. oxytoca.
Key words: 1-amino-cyclopropane-1-carboxylic acid, ACC deaminase, PCR, rhizobacteria, Striga hermonthica.
Olubukola O. Babalola*, Ellie O. Osir, George D. Odhiambo, and Wallace D. Bulimo, Abiodun I. Sanni
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD)
Bahy Ahmed Ali
Nucleic Acid Research Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), Mubarak City For Scientific Research & Technology Applications, Alexandria, Egypt. E-mail: [email protected] or [email protected], Fax: 203 4593323
Accepted 12 May 2003
Abstract
RAPD technique was used in this study to detect DNA band variations between both normal and abnormal male of broiler chicken based on RAPD marker. DNA polymorphisms between normal and mutant birds were detected using fifteen oligonucleiotide primers. Using these primers, DNA band loss ranged from 25 to 75%. Data demonstrated that RAPD marker could detect DNA alterations.
Keywords: DNA alteration, RAPD, abnormal phenotype, male, broiler chicken.
Bahy Ahmed Ali
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob
OJUMU, Tunde Victor1*, SOLOMON, Bamidele Ogbe2,e, BETIKU, Eriola2, , LAYOKUN, Stephen Kolawole 2, and AMIGUN, Bamikole3
1Engineering Materials Development Institute, P.M.B 611, Akure Nigeria.
2Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria.
3Department of Food Science and Technology, Federal Polytechnic Ado-Ekiti, Ekiti State, Nigeria.
Accepted 23 May 2003
Abstract
Bagasse, corncob and sawdust were used as lignocellulosic substrates for the production of cellulase enzyme using Aspergillus flavus after ballmilling and pretreatment with caustic soda. From the fermentation studies, sawdust gave the best result with an enzyme activity value of 0.0743IU/ml while bagasse and corncob gave 0.0573IU/ml and 0.0502IU/ml respectively. The three lignocellulosics gave their maximum enzyme activities at about the twelfth hour of cultivation, suggesting that the 12th hour is the optimum time when the enzyme may be harvested.
Key words: Aspergillus flavus, cellulase activity, lignocellulosics.
Stephen Kolawole and AMIGUN, SOLOMON , Tunde Victor*, OJUMU , Bamikole , BETIKU , LAYOKUN , Bamidele Ogbe, Eriola
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Production of poly- -hydroxybutyrate (PHB) and differentiation of putative Bacillus mutant strains by SDS-PAGE of total cell protein
Hikmet Katırcıoğlu1*, Belma Aslım2, Zehra Nur Yüksekdað2, Nazime Mercan3, Yavuz Beyatlı2
1*Department of Biology Education, Gazi University, Ankara, Turkey
2Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey
3Department of Biology, Faculty of Science, Pamukkale University, Denizli, Turkey
Accepted 16 May 2003
Abstract
In this study, the putative mutant strains of Bacillus megaterium Y6, B. subtilis K8, B. sphaericus X3 and B. firmus G2 were studied for their poly- -hydroxybutyrate (PHB) production capacities. Mutations were induced by using UV light, acriflavin and 5-bromourasil. Total cell proteins were extracted from 59 strains and compared using SDS-PAGE. For each strain, percentage yield of PHB according to cell dry weight was determined in a range of 1.46-63.45%. PHB production of 8 mutant strains were found to increase in comparison with parental strains. However, no increase in PHB production of mutant strains of B. sphaericus X3 was found. It was also determined that the protein profiles of the mutant strains with high PHB yield generally differed from the protein profiles of parental strains.
Key words: Bacillus, poly- -hydroxybutyrate, PHB, total cell protein.
Zehra Nur Yüksekdað, Hikmet Katırcıoğlu*, Belma Aslım, Nazime Mercan, Yavuz Beyatlı
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Bacillus pumilus BpCRI 6, a promising candidate for cellulase production under conditions of catabolite repression
Kotchoni O.S.1*†, Shonukan O.O.1 and Gachomo W.E.2
1Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
2Institute for Plant Diseases, University of Bonn, Nussallee 9, D-53115 Germany.
Accepted 16 May 2003
Abstract
Cellulose degrading organisms have been used for the conversion of cellulolytic materials into soluble sugars or solvents in several biotechnological and industrial applications. In this report, a mutant of Bacillus pumilus was obtained after chemical mutagenesis and screened for cellulase production. This mutant named BpCRI 6 was selected for its ability to produce cellulase under catabolite repression. Cellulase yield by BpCRI 6 was four times higher than that of the wild type under optimum growth conditions (pH 6.5, 25°C and Ca2+ 1mM). In shaking flask cultures, production of cellulase by the wild type was completely repressed in the presence of 25 mM glucose, while BpCRI 6 strain still exhibited a residual cellulase production of 80 and 40% at 25 mM and 40 mM of glucose concentrations respectively. The mutant strain is stable and grows rapidly in liquid and solid media. Under conditions of catabolite repression (40 mM of glucose), the production of cellulase by this mutant is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition. This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus.
Key words: Cellulase, Bacillus pumilus, BpCRI 6, Catabolite repression.
Kotchoni O.S, Shonukan O.O and Gachomo W.E
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
DNA Sequences of RAPD Fragments in the Egyptian cotton Gossypium barbadense
Abdel Ghany A. Abdel Ghany1 and Essam A. Zaki2,*
1Institute of Efficient Productivity, Zagazig University, Zagazig, Egypt.
2Genetic Engineering and Biotechnology Research Institute, GEBRI, Research Area, Borg El Arab, Post Code 21934, Alexandria, Egypt.
Accepted 4 April, 2003
Abstract
Random Amplified Polymorphic DNAs (RAPDs) is a DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. Despite the fact that the RAPD technique has become a very powerful tool and has found use in numerous applications, yet, the nature of molecular variation(s) uncovered by the RAPD technique is still unclear. The aim of the following study, therefore, was to investigate the molecular nature of RAPD DNA fragments in four Gossypium barbadense cultivars. Five RAPD DNA fragments, generated by improved RAPD-PCR technique, and representing polymorphic and nonpolymorphic bands were analyzed at the molecular level using DNA sequence analysis. Nonpolymorphic RAPD DNA fragments showed homologies to previously characterized plant structural genes. Comparative nucleotide sequence analysis of two comigrating nonpolymorphic fragments revealed that these two DNA sequences are highly similar to each other, indicating that similarity of fragment size is a good predicator of homology. Polymorphic RAPD DNA fragments, on the other hand, showed homologies to middle and high-repetitive DNA sequences. These results promote the initiative to integrate these RAPD markers in cotton breeding applications, and DNA fingerprinting.
Key words: Gossypium, MITEs, RAPD-PCR, repetitive DNA, sequence similarity, retrotransposons.
Abdel Ghany A. Abdel Ghany, Essam A. Zaki
Page: 1 - 10