Advanced Journal of Microbiology Research

ISSN 2736-1756

Table of Contents 2019

Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals

Full Length Research Paper

Purification and characterization of bacteriocin isolated from Streptococcus thermophilus

Aslam M.1*, Shahid M.1, Rehman F. U.2, Naveed N. H.3, Batool A. I.3, Sharif S.1 and Asia A.1,

1Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan.

2Department of Chemistry, University of Sargodha, Sargodha, Pakistan.

3Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan.

Accepted 11 February, 2019

Abstract

Streptococcus thermophilus is used primarily as starter cultures to counter the harmful bacteria grown in cheese and yogurt making/preservation processes. These bacteria produce some exogenous toxins called bacteriocins having the antimicrobial activities against both Gram positive and Gram negative bacteria. In our study S. thermophilus growth was obtained at pH 5.5 and temperature 40°C. Bacteriocin activities were checked after their treatment with different enzymes, organic solvents, sodium chloride (NaCl) and detergents as well as their heat stability and effect of pH was studied. Bacteriocin activity was found heat stable at 100°C for 30 min and was found stable in the 3-10 pH range but lost the activity after the treatment with proteinase-K and protease enzymes. Activity was lost in treatment with lipase and amylase which shows the presence of lipo-glycolated peptide. Bacteriocin activity was lost on the presence of Dithiothreitol (DTT) and β-mercaptoethanol which showed the presence of disulphide bond present in bacteriocin and essential for its activity. Urea and ethylene diamine tetraacetic acid (EDTA) also affected the bacteriocin activity but found stable to survive in the presence of 6% NaCl. Antibacterial assay showed the strong growth inhibition of test bacteria. Bactericidal activity was further purified to homogeneity by ammonium sulphate precipitation and different chromatographic techniques. Molecular weight was calculated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as 2.7 kDa.

Key words: Streptococcus thermophilus, bacteriocin, antimicrobial peptide, yogurt culture bacteriocin, disc diffusion test.

Shahid M, Aslam M*, Rehman F. U, Naveed N. H, Batool A. I, Sharif S and Asia A

Page: 1 - 7

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-006, April, 2019. © International Scholars Journals

Full Length Research Paper

The domestic wastewater (DWW) influence on secondary metabolite in thyme (Thymus vulgaris L.) under field conditions

Hossein Hassanpour Darvishi1 and Hossein Aliabadi Farahani2

1Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran.

2Young Researchers Club, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.

Accepted 13 February, 2019

Abstract

In order to study the domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters were irrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary drainage water and then, were accumulated by secondary drainage water and we have irrigation 10, 11 and 12 lysimeters by secondary drainage water. In order to compare plants characteristics, in 13, 14 and 15 lysimeters were irrigated by agronomical water. The secondary metabolite in thyme 3.4% increased under irrigation by secondary drainage water into irrigation by agronomical water. The findings may give applicable advice to medicinal and aromatic plants researchers for management and concern on water strategy and estimate of irrigation carefully for increase of quantity and quality yields in medicinal and aromatic plants farming.

Key words: Domestic wastewater, essential oil yield, Thymus vulgaris.

Full Length Research Paper 

 

The domestic wastewater (DWW) influence on secondary metabolite in thyme (Thymus vulgaris L.) under field conditions 

 

Hossein Hassanpour Darvishi1 and Hossein Aliabadi Farahani2 

 

1Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran. 

2Young Researchers Club, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. 

 

Accepted 13 February, 2019 

 

In order to study the domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters were irrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary drainage water and then, were accumulated by secondary drainage water and we have irrigation 10, 11 and 12 lysimeters by secondary drainage water. In order to compare plants characteristics, in 13, 14 and 15 lysimeters were irrigated by agronomical water. The secondary metabolite in thyme 3.4% increased under irrigation by secondary drainage water into irrigation by agronomical water. The findings may give applicable advice to medicinal and aromatic plants researchers for management and concern on water strategy and estimate of irrigation carefully for increase of quantity and quality yields in medicinal and aromatic plants farming. 

 

Key words: Domestic wastewater, essential oil yield, Thymus vulgaris. 

 

 

INTRODUCTION 

 

Wastewater is any water that has been adversely affected in quality by anthropogenic influence. It com-prises liquid waste discharged by domestic residences, commercial properties, industry, and or agriculture and can encompass a wide range of potential contaminants and concentrations. In the most common usage, it refers to the municipal wastewater that contains a broad spectrum of contaminants resulting from the mixing of wastewaters from different sources. Sewage is correctly the subset of wastewater that is contaminated with feces or urine, but is often used to mean any waste water (Mittler, 2002). "Sewage" includes domestic, municipal, or industrial liquid waste products disposed of, usually via a pipe or sewer or similar structure, sometimes in a cesspool emptier. The physical infrastructure, including 

 
 

 

 

 

 


*Corresponding author. E-mail: [email protected]. 

 

Full Length Research Paper
The domestic wastewater (DWW) influence on secondary metabolite in thyme (Thymus vulgaris L.) under field conditions
Hossein Hassanpour Darvishi1 and Hossein Aliabadi Farahani2
1Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran.
2Young Researchers Club, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
Accepted 13 February, 2019
In order to study the domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters were irrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary drainage water and then, were accumulated by secondary drainage water and we have irrigation 10, 11 and 12 lysimeters by secondary drainage water. In order to compare plants characteristics, in 13, 14 and 15 lysimeters were irrigated by agronomical water. The secondary metabolite in thyme 3.4% increased under irrigation by secondary drainage water into irrigation by agronomical water. The findings may give applicable advice to medicinal and aromatic plants researchers for management and concern on water strategy and estimate of irrigation carefully for increase of quantity and quality yields in medicinal and aromatic plants farming.
Key words: Domestic wastewater, essential oil yield, Thymus vulgaris.

Hossein Hassanpour Darvishi, Hossein Aliabadi Farahani

Page: 1 - 6

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals

Full Length Research Paper

Influence of microbial inoculants on soil response to properties with and without straw under different temperature regimes

Shaukat Abro1, Xiaohong Tian2*, Donghai You1, Yuling Ba2, Meng Li2, and Faqi Wu2

1College of Resources and Environment, Northwest University, China.

2College of Resources and Environment, Open Laboratory for Plant Nutrition and Agricultural Resources and in Northwest of Agriculture Ministry of China, Yangling, 712100 Shaanxi, P. R. China.

Accepted 12 March, 2019

Abstract

A laboratory study was conducted to investigate efficacy of locally produced microbial inoculants with different concentrations on microbial respiration from soils with and without addition of wheat straw incubated at 20 and 25°C temperatures for 73 days. The results indicated that the soil microbial community structure and activities were obviously influenced by microbial inoculants. In general, whereas all inoculants levels were successfully established to different extents on the straw, none significantly improved decomposition in either soils or straw amended treatments. Additions of microbial inoculants expedite the decomposition and CO2 evolution process. CO2-C emission was regularly determined and results revealed microbial inoculants had little (10%) while straw addition significantly increased decomposition rates and cumulative CO2-C by 39% in straw amended than controls. Straw addition into soil had significant relationships (p < 0.05) with cumulative amounts of C; soil organic C, microbial biomass nitrogen. There was highly significant relationship between CO2-C emission and incubation period (R2=0.94) . In conclusion, microbial inoculants and straw incorporation will enhance CO2-C evolution, soil carbon and microbial biomass, thus improving soil quality.

Key words: Microbial inoculants, soil CO2-C evolution, decomposition microbial biomass, organic carbon.

Shaukat Abro, and Faqi Wu, Donghai You, Xiaohong Tian*, Yuling Ba, Meng Li

Page: 1 - 8

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals

Full Length Research Paper

Assessing the potential of Tetradenia riparia in treatment of common skin conditions in rural communities of South Africa

R. M. Coopoosamy* and K. K. Naidoo

Department of Nature Conservation, Mangosuthu University of Technology, P. O. Box 12363, Jacobs, 4026, Durban, KwaZulu-Natal, South Africa.

Accepted 20 March, 2019

Abstract

Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in treatment of secondary infections, such as, mouth sores, pelvic or vaginal sores in individuals who are HIV/AIDS infected.

Key words: Tetradenia riparia, iboza, antimicrobial, traditional medicines.

R. M. Coopoosamy*, K. K. Naidoo

Page: 1 - 4

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Research Article

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals

Full Length Research Paper

A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII  T30C

Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2

1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.

2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.

Accepted 03 March, 2019

Abstract

Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.

Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.

Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar

Page: 1 - 7

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Review

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals

Review

Chromobacterium violaceum infection in China: Three case reports and literature reviews

Tai Ma1, Wei Shi3, Jun Cheng1, Jian-Kang Zhang1, Li-Fen Hu1, Ying Ye1,2 and Jia-Bin Li1,2*

1Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, 230022, Hefei, Anhui Province, China.

2Anhui Center for Surveillance of Bacterial Resistance, 230022, Hefei, Anhui Province, China.

3Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060; China.

Accepted 22 January, 2019

Abstract

Chromobacterium violaceum, Gram-negative Bacillus, is a common inhabitant of soil and stagnant water found in tropical and subtropical regions of the world. It is a rare cause of severe, often fatal, human disease. In the report, 3 cases of patients infected with C. violaceum were described in Anhui Province, China. Routine and bacteriological investigations were carried out to establish the aetiological diagnosis. Moreover, the patients were treated with appropriate antimicrobial agents and auxiliary therapy. To our knowledge, a total of 42 cases have been reported previously from Chinese mainland in the recent 20 years, with a review of the literatures.

Key words: Chromobacterium violaceum, infection, China.

Jian-Kang Zhang, Li-Fen Hu, Wei Shi, Ying Ye and Jia-Bin Li*, Tai Ma, Jun Cheng

Page: 1 - 8

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