ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2015
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2015 International Scholars Journals
Full Length Research Paper
Screening and optimization of nutrients for L-asparaginase production by Bacillus cereus MNTG-7 in SmF by plackett- burmann design
M. Sunitha*, P. Ellaiah and R. Bhavani Devi
Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003, India.
Accepted 17 December, 2014
Abstract
The medium components influencing the enzyme production were screened using Plackett-Burman design. A total of 67 nutrients comprising of fifteen each of carbon, nitrogen, mineral/salt and eleven each of inorganic nitrogen sources and amino acid sources were screened for the production of L-asparaginase by SmF. This design involves screening of up to ‘n-1’ variables in just ‘n’ number of experiments. Regression coefficients and t-values were calculated by subjecting the experimental data to statistical analysis. Among the 67 nutrients based on their performance in terms of product yield and cost, tapioca starch, L-asparagine, ammonium oxalate, gelatin and CaCO3 were identified as most effective and therefore selected for inclusion in surface methodology studies.
Key words: L-asparaginase, nutritional factors, submerged fermentation, Bacillus cereus MNTG-7.