ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
Influence of microbial inoculants on soil response to properties with and without straw under different temperature regimes
Shaukat Abro1, Xiaohong Tian2*, Donghai You1, Yuling Ba2, Meng Li2, and Faqi Wu2
1College of Resources and Environment, Northwest University, China.
2College of Resources and Environment, Open Laboratory for Plant Nutrition and Agricultural Resources and in Northwest of Agriculture Ministry of China, Yangling, 712100 Shaanxi, P. R. China.
Accepted 12 March, 2019
Abstract
A laboratory study was conducted to investigate efficacy of locally produced microbial inoculants with different concentrations on microbial respiration from soils with and without addition of wheat straw incubated at 20 and 25°C temperatures for 73 days. The results indicated that the soil microbial community structure and activities were obviously influenced by microbial inoculants. In general, whereas all inoculants levels were successfully established to different extents on the straw, none significantly improved decomposition in either soils or straw amended treatments. Additions of microbial inoculants expedite the decomposition and CO2 evolution process. CO2-C emission was regularly determined and results revealed microbial inoculants had little (10%) while straw addition significantly increased decomposition rates and cumulative CO2-C by 39% in straw amended than controls. Straw addition into soil had significant relationships (p < 0.05) with cumulative amounts of C; soil organic C, microbial biomass nitrogen. There was highly significant relationship between CO2-C emission and incubation period (R2=0.94) . In conclusion, microbial inoculants and straw incorporation will enhance CO2-C evolution, soil carbon and microbial biomass, thus improving soil quality.
Key words: Microbial inoculants, soil CO2-C evolution, decomposition microbial biomass, organic carbon.
Shaukat Abro, and Faqi Wu, Donghai You, Xiaohong Tian*, Yuling Ba, Meng Li
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-005, April, 2019. © International Scholars Journals
Full Length Research Paper
Prevalence of bacterial and Candida albicans infection amongst women attending Irrua Specialist Teaching Hospital, Irrua, Nigeria
Isibor, J. O.1, Samuel, S. O.2*, Nwaham, C. I.3, Amanre I. N.1, Igbinovia, O.4 and Akhile, A. O.3
1Department of Microbiology, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
2Department of Medical Microbiology, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
3Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
4Department of Community Medicine, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Accepted 19 January, 2019
Abstract
Bacterial and other pathogens often infect the female genital tract causing disease in most women, sometimes with severe complications. A number of vaginal infections present with few or no symptoms and yet could still be transmissible to sexual partners of infected persons. Poor socio- economic status, lack of diagnostic facilities and shortage of effective treatment all contribute to the high incidence of sexually transmitted and reproductive tract infections. In this study we investigated mixed bacterial and Candida albicans infections in antenatal and gynecology attendees in Irrua Specialist Teaching Hospital Irrua, Edo State, Nigeria. Seventy-five high vaginal swab specimens were collected from female patients (18- 49 years) attending ante-natal and gynecology clinics of the hospital. Specimens were collected from symptomatic and asymptomatic patients and were analyzed using standard microbiological methods. Wet preparations were examined microscopically for presence of white blood cells (WBC) which suggested an infection, and yeast cells. The swabs were inoculated on MacConkey, Blood, and Chocolate agars, incubated at 37°C for 24 h, as well as Sabouraud Dextrose agar, incubated at room temperature for a few days. Of the seventy-five specimens analyzed, 56 (74.7%) isolates were from symptomatic patients while 25 (44.6%) were from asymptomatic patients. C. albicans was the most isolated pathogen with 35 (47.7%) isolates, followed by Staphylococcus aureus with 25 (29.8%), E. coli, 11 (13.1%), Klebsiella spp, 5 (6.0%) Enterococcus faecalis, 4(4.8%), Proteus spp 3 (3.6%) and Pseudomonas aeruginosa, 1(1.2%). This analysis revealed that candidiasis caused by C. albicans and infection with S. aureus is still the major health problems among females in this locality. The need for regular check- up at the clinics as well as personal hygiene is highly recommended in order to forestall avoidable infections.
Key words: Bacteria, Candida albicans, infection, antenatal, gynecology, attendees, Irrua, Nigeria.
S. O*, C. I, Igbinovia , Isibor , J. O, Nwaham , O and Akhile, Amanre I. N, A. O, Samuel
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-005, April, 2019. © International Scholars Journals
Full Length Research Paper
Cytotoxicity and antimicrobial activity of Teucrium scordium L. (Lamiaceae) extracts
Tatjana Kundakovi 1*, Marina Milenkovi 2, Aleksandra Topi 3, Tatjana Stanojkovi 4, Zorica Jurani 4 and Branislava Lakuši 5
1Department of Pharmacognosy, Faculty of Pharmacy, 11000 Belgrade, Serbia.
2Department of Microbiology and Immunology, Faculty of Pharmacy, 11000 Belgrade, Serbia.
3Department of Medical Biochemistry, Faculty of Pharmacy, 11000 Belgrade, Serbia.
4Institute for Oncology and Radiology of Serbia, 11000 Belgrade, Serbia.
5Department of Botany, Faculty of Pharmacy, 11000 Belgrade, Serbia.
Accepted 03 April, 2019
Abstract
Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB- 361 cells (IC50=130.33±0.1 g/ml and IC50=189.89±3.99 g/ml, respectively). Dichlormethane extract was more effective against MDA-MB-453 cell line (IC50=130.33 ± 0.1 g/ml). The methanol extract of T. scordium possessed no cytotoxicity against breast cancer cell lines, MDA-MB-361 and MDA-MB-453. Herb extracts of T. scordium have shown weak antibacterial activity on Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis with no activity against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Enterococcus faecalis, and Candida albicans.
Key words: Teucrium scordium subsp. scordioides, cytotoxicity, antimicrobial activity.
Zorica Jurani and Branislava Lakuši, Tatjana Stanojkovi, Aleksandra Topi, Tatjana Kundakovi*, Marina Milenkovi
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
Theoretical studies on nanostructure of 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde
M. Monajjemi1*, F. Naderi2, F. Jadidi3 and F. Mollaamin4
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of basic science, Shahr e Qods branch, Islamic Azad University, Iran.
3Science and Research Branch, Islamic Azad University, Tehran, Iran.
4Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran.
Accepted 15 March, 2019
Abstract
The polarized continuum model (PCM) model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in solutions. The maximum IR intensity is related to the C4=C5 stretching vibrations. Also, the vibrational free energy, heat capacity, entropy, thermal energy and zero point vibrational energy obtained from the calculated frequencies and these are compared in different media.
Key words: Polarized continuum model (PCM), functional theory methods (DFT), solvent, IR, safranal.
F. Jadidi and F. Mollaamin, F. Naderi, M. Monajjemi*
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Assessing the potential of Tetradenia riparia in treatment of common skin conditions in rural communities of South Africa
R. M. Coopoosamy* and K. K. Naidoo
Department of Nature Conservation, Mangosuthu University of Technology, P. O. Box 12363, Jacobs, 4026, Durban, KwaZulu-Natal, South Africa.
Accepted 20 March, 2019
Abstract
Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in treatment of secondary infections, such as, mouth sores, pelvic or vaginal sores in individuals who are HIV/AIDS infected.
Key words: Tetradenia riparia, iboza, antimicrobial, traditional medicines.
R. M. Coopoosamy*, K. K. Naidoo
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII T30C
Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2
1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.
2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.
Accepted 03 March, 2019
Abstract
Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.
Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar
Page: 1 - 7