ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII T30C
Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2
1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.
2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.
Accepted 03 March, 2019
Abstract
Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.
Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238
Farah Nadia Omar, Nor’Aini Abdul Rahman*, Halimatun Saadiah Hafid, Tabassum Mumtaz, Phang Lai Yee and Mohd Ali Hassan
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Accepted 11 March, 2019
Abstract
Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain was achieved at the highest PHB content of 52.79% in batch fermentation using the kitchen-waste derived organic acids. The PHB yield and productivity were 0.38 g/g and 0.065 g/L/h, respectively. In fed-batch culture, about 4-fold increase in PHB productivity (0.242 g/L/h) was achieved by applying intermittent feeding strategy.
Key words: Cupriavidus necator CCGUG 52238, kitchen waste, organic acids, polyhydroxybutyrate (PHB).
Nor’Aini Abdul Rahman*, Farah Nadia Omar, Halimatun Saadiah Hafid, Phang Lai Yee and Mohd Ali Hassan, Tabassum Mumtaz
Research Article
Theoretical studies on nanostructure of 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
Theoretical studies on nanostructure of 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde
M. Monajjemi1*, F. Naderi2, F. Jadidi3 and F. Mollaamin4
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of basic science, Shahr e Qods branch, Islamic Azad University, Iran.
3Science and Research Branch, Islamic Azad University, Tehran, Iran.
4Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran.
Accepted 15 March, 2019
Abstract
The polarized continuum model (PCM) model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in solutions. The maximum IR intensity is related to the C4=C5 stretching vibrations. Also, the vibrational free energy, heat capacity, entropy, thermal energy and zero point vibrational energy obtained from the calculated frequencies and these are compared in different media.
Key words: Polarized continuum model (PCM), functional theory methods (DFT), solvent, IR, safranal.
F. Jadidi and F. Mollaamin, F. Naderi, M. Monajjemi*
Research Article
Molecular characterization of norovirus from acute gastroenteritis patients in Malaysia
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Molecular characterization of norovirus from acute gastroenteritis patients in Malaysia
H. Zuridah1,2*, Sufiyan Hadi M1, L. K. Teh2 and A. H. Zed Zakari1
1Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
2Pharmacogenomic Centre (PROMISE), Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
Accepted 11 February, 2019
Abstract
Rotavirus and recently norovirus have been described as important and most common cause of acute gastroenteritis in children. The mode of transmission is fecal-oral. Diagnosis of both of these viruses can be made by rapid antigen detection of the viruses in stool specimens and strains can be further characterized by enzyme immunoassay or reverse transcriptase polymerase chain reaction. Stool specimens collected from various hospitals in Malaysia were examined for norovirus by both immunochromatography and reverse-transcription polymerase chain reaction techniques. Rotavirus antigen was screened by a commercially available latex agglutination test kit. Altogether, 168 stool samples were collected for both norovirus and rotavirus screening. Out of these, 77/168 (45.8%) were examined for rotavirus with 17/77 (22%) rotavirus antigen positive. Due to very small amount of fecal materials obtained in some cases, only 151/168 (89.8%) were sufficient for norovirus and 14/151 (9.3%) were positive for norovirus genogroup II (GII). This study highlights that rotavirus remains the main agent for acute gastroenteritis and identification for emerging norovirus among the children is becoming important for proper patient management.
Key words: Norovirus, rotavirus, immunochromatography, latex agglutination, RT-PCR, phylogenetic analysis.
H. Zuridah*, Sufiyan Hadi M, L. K. Teh and A. H. Zed Zakari
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
Influence of microbial inoculants on soil response to properties with and without straw under different temperature regimes
Shaukat Abro1, Xiaohong Tian2*, Donghai You1, Yuling Ba2, Meng Li2, and Faqi Wu2
1College of Resources and Environment, Northwest University, China.
2College of Resources and Environment, Open Laboratory for Plant Nutrition and Agricultural Resources and in Northwest of Agriculture Ministry of China, Yangling, 712100 Shaanxi, P. R. China.
Accepted 12 March, 2019
Abstract
A laboratory study was conducted to investigate efficacy of locally produced microbial inoculants with different concentrations on microbial respiration from soils with and without addition of wheat straw incubated at 20 and 25°C temperatures for 73 days. The results indicated that the soil microbial community structure and activities were obviously influenced by microbial inoculants. In general, whereas all inoculants levels were successfully established to different extents on the straw, none significantly improved decomposition in either soils or straw amended treatments. Additions of microbial inoculants expedite the decomposition and CO2 evolution process. CO2-C emission was regularly determined and results revealed microbial inoculants had little (10%) while straw addition significantly increased decomposition rates and cumulative CO2-C by 39% in straw amended than controls. Straw addition into soil had significant relationships (p < 0.05) with cumulative amounts of C; soil organic C, microbial biomass nitrogen. There was highly significant relationship between CO2-C emission and incubation period (R2=0.94) . In conclusion, microbial inoculants and straw incorporation will enhance CO2-C evolution, soil carbon and microbial biomass, thus improving soil quality.
Key words: Microbial inoculants, soil CO2-C evolution, decomposition microbial biomass, organic carbon.
Shaukat Abro, and Faqi Wu, Donghai You, Xiaohong Tian*, Yuling Ba, Meng Li
Review
Chromobacterium violaceum infection in China: Three case reports and literature reviews
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Review
Chromobacterium violaceum infection in China: Three case reports and literature reviews
Tai Ma1, Wei Shi3, Jun Cheng1, Jian-Kang Zhang1, Li-Fen Hu1, Ying Ye1,2 and Jia-Bin Li1,2*
1Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, 230022, Hefei, Anhui Province, China.
2Anhui Center for Surveillance of Bacterial Resistance, 230022, Hefei, Anhui Province, China.
3Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060; China.
Accepted 22 January, 2019
Abstract
Chromobacterium violaceum, Gram-negative Bacillus, is a common inhabitant of soil and stagnant water found in tropical and subtropical regions of the world. It is a rare cause of severe, often fatal, human disease. In the report, 3 cases of patients infected with C. violaceum were described in Anhui Province, China. Routine and bacteriological investigations were carried out to establish the aetiological diagnosis. Moreover, the patients were treated with appropriate antimicrobial agents and auxiliary therapy. To our knowledge, a total of 42 cases have been reported previously from Chinese mainland in the recent 20 years, with a review of the literatures.
Key words: Chromobacterium violaceum, infection, China.
Jian-Kang Zhang, Li-Fen Hu, Wei Shi, Ying Ye and Jia-Bin Li*, Tai Ma, Jun Cheng