ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (9), pp. 001-007, September, 2018. © International Scholars Journals
Full Length Research Paper
A microbiological technique for the separation of Hibiscus sabdariffa L. fibers
Pilanee Vaithanomsat1*, Poom Phusanakom2, Waraporn Apiwatanapiwat1 and Molnapat Songpim1
1Enzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok 10900, Thailand.
2Toyota Motor Asia Pacific Engineering and Manufacturing Company Limited, 99 Moo 5, Ban Ragad, Amphur Bang Bo, Samutprakan 10560, Thailand.
Accepted 29 June 2018
Abstract
This study is related to a process for the separation of Hibiscus sabdariffa L. fibers using living microorganisms. The selected fungal strains, Datronia sp. and Oligoporous sp., were isolated from natural sources in Thailand and were previously shown to be capable of degrading lignin in pulp and paper industrial wastewater. The process described here is comprising the fungal-treated fibrous of H. sabdariffa L. barks in certain conditions. The fungal inoculum size, retting time and retting temperature were important in the experiments and the retting efficiency was based on the visual characteristics of resulting fibers. In addition, the pectinolytic enzymes released from the fungi in retting conditions were shown to correspond to the separation of Hibiscus sabdariffa L. fibers.
Key words: Hibiscus sabdariffa L., fibers, separation, microbiological process.
Poom Phusanakom, Waraporn Apiwatanapiwat and Molnapat Songpim, Pilanee Vaithanomsat*
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (9), pp. 001-006, September, 2018. © International Scholars Journals
Full Length Research Paper
Simple method to study the mechanism of thermal and non thermal bactericidal action of microwave radiations on different bacterial species
Arifa Tahir1*, Bushra Mateen1, Salih Univerdi2, Ozer KaraGoban2 and Mehmet Zengin2
1Microbiology Research Centre, Lahore College for Women University, Lahore Pakistan.
2Science department, PakTurk International Colleges, Islamabad, Lahore Pakistan.
Accepted 13 July 2018
Abstract
The present study was based on the investigations to determine whether the bactericidal effect of microwave radiations on bacteria was either thermal or due to microwaves. It was also investigated which bacterial sp is more sensitive to microwave. Five bacterial species, Proteus vulgaris PP25D, Staphylococcus aureus bgh010, Bacillu subtilis ase98, Escherchia coli TRE04 and Corynebacterium spN33 were exposed to microwave radiation. These cultures were selected as test organisms due to their importance in food industry as human pathogens. Bacterial suspensions were exposed to microwave radiations (2450 MHz and 800W) for 60, 120 and 180 s to study the effect of heat generated by microwaves. The second experiment was designed to study the effect of microwaves only by maintaining temperature below 40°C. The degree of inactivation at uncontrolled and controlled temperature was compared quantitavely. The viable counts of all cell suspensions were found to reduce greatly with an increase in microwave heating time and temperature. B. subtilis ase98 showed highest reduction at uncontrolled temperature. No significant reduction of cell density was observed in either cell suspension. The effect of microwave radiations was also studied in terms of morphological changes. No detectable change was observed in cell shape and morphology of colonies except S. aureus bgh010. Results indicated that the effect of microwave radiations on bacteria was purely thermal and no detectable change occurred in non-thermal treatment. B. subtilis ase98 were the most sensitive species for microwave radiations. B. subtilis ase98 can be used as an indicator bacterium to assess microwaves for sterilization.
Key words: Microwave radiation, thermal and non thermal effect, sensitive bacterial sp.
Salih Univerdi, Bushra Mateen, Arifa Tahir*, Ozer KaraGoban and Mehmet Zengin
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (9), pp. 001-007, September, 2018. © International Scholars Journals
Full Length Research Paper
Comparison of Norwegian poultry, waterborne and clinical isolates of Campylobacter jejuni by ribotyping
Olav Rosef1*, Algimantas Paulauskas2, Nils Grude3, Camilla Haslekås1 and Andrew Jenkins3
1Telemark University College, Hallvard Eikas Plass 1, 3800 Bø i Telemark, Norway.
2Vytautas Magnus University, Kaunas, Lithuania.
3Unilabs Telelab AS, Skien, Norway.
Accepted 20 July, 2018
Abstract
Campylobacteriosis is the most frequently occurring food and waterborne gastroenteritis in Norway and 90% of cases are caused by Campylobacter jejuni. Campylobacteriosis is epidemiologically associated with use of non-disinfected water and consumption of contaminated poultry. In order to provide molecular epidemiological support for this association isolates from local poultry and water were compared with human isolates from domestic and import infections using ribotyping. Automatic riboprints were performed with the PstI restriction enzyme and RiboPrinter® and compared with a library of recognised patterns (DUP-IDs). Patterns were further compared with each other using GelCompar software. The isolates from human clinical cases showed high heterogeneity. DUP-IDs found among human isolates were also found in 2 isolates from poultry, DUP-PSTI-1146 and DUP-PSTI-2061. Two human isolates were like isolates from water, DUP-PSTI-2073 and DUP-PSTI-1122, but these were from patients infected abroad. This study provides limited support for the importance of poultry as a source of infection and illustrates the need for studies of much larger scope to encompass the huge diversity of strains and sources of C. jejuni.
Key words: Campylobacter, similarity, clustering, genotyping, riboprint.
Nils Grude, Camilla Haslekås and Andrew Jenkins, Olav Rosef*, Algimantas Paulauskas
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (8), pp. 001-011, August, 2018. © International Scholars Journals
Full Length Research Paper
Structural organization of co-regulated genes in Escherichia coli
Ali Azam Talukder1-3, 5*, Saidul Alam2, Md Ershaduzzaman4, Sam Khairul Bashar3 and Mamoru Yamada5
1Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
2Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
3Department of Life Sciences, North South University, Plot 15, Block B, Bashundhara R/A, Dhaka 1229, Bangladesh.
4Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh.
5Department of Biological Chemistry, Yamaguchi University, Yamaguchi 753-8515, Japan.
Accepted 25 May, 2018
Abstract
A promoter-protein fusion library was constructed and analyzed previously which covered about 8% promoter-proximal genes from Escherichia coli in respect to their reading frames, protein productivity as well as expressional regulations under normal and various environmental stress conditions (Talukder et al., 1994, Bioscience Biochemistry and Biotechnology, 58: 117-120). In this study, we further analyzed 13 significantly responsive genes. -galactosidase expression levels were varied among the examined clones, indicating that the library having different ranges of promoters from stronger to weaker. DNA database analysis revealed that out of 13 genes studied here, 11 and 2 genes were found to known and unknown or new genes, respectively. Moreover, new findings in case of three genes having interesting structural organizations were recorded this time. The present study supports all previous data to conclude that about 5 - 10% E. coli genes are co-regulated under the control of complex regulatory circuits.
Key words: Gene expression, RNA-polymerase, gene organization.
Sam Khairul Bashar and Mamoru Yamada, Md Ershaduzzaman, Ali Azam Talukder*, Saidul Alam
Page: 1 - 11
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (8), pp. 001-005, August, 2018. © International Scholars Journals
Full Length Research Paper
Evaluation of in-vitro inhibitory effect of honey on some microbial isolate
Raied Taha Al- Naama
Department of Microbiology, College of Dentistry, University of Basrah, Iraq. E-mail: [email protected]. Tel:
009647801298217.
Accepted 24 June, 2018
Abstract
The aim of this study was to investigate the antimicrobial activity of honey sample from Basrah region against certain microbial isolate. Different concentrations (25.0, 50.0, 75.0 and 100.0%) of honey sample where checked for their antimicrobial activities, using some medically important micro-organisms including Escherichia coli, Pseudomonas spp. and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) of the honey sample were determined on the selected micro-organisms by using broth dilution technique. The sample of honey show inhibitory effect in vitro at 50, 75 and 100% concentration on the various investigated micro-organism except at 50% concentration where no inhibition zone on S. aureus. However, no effect was observed at 25% concentration. The MIC for E. coli, Pseudomonas spp. and S. aureus were 6.25, 1.5 and 12.5 mg/ml respectively. The study shows that honey, like antibiotics, has certain organisms sensitive to it, and provides alternative therapy against certain bacteria and is also shown to have antimicrobial action against a broad spectrum of bacteria (both gram- positive and -negative bacteria).
Key words: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, honey, antibiotics, sensitivity, antimicrobial.
Raied Taha Al- Naama
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (8), pp. 001-004, August, 2018. © International Scholars Journals
Full Length Research Paper
Isolation of Enterobacteriaceae and Pseudomonas spp. from raw fish sold in fish market in Khartoum state
Sanaa O. Yagoub
Department of Microbiology and Molecular Biology, Faculty of Science and Technology, El Neelain University, P. O. Box 12702, Khartoum, Sudan. E-mail: soyagoub08@ hotmail.com. Fax: 00249-83-776338.
Accepted 22 April, 2018
Abstract
Enterobacteriaceae were isolated from gills, skin, muscles and the intestine of 83 out of 150 (55%) randomly collected fishes, the most dominants isolates were E. coli, Citrobacter spp, Enteriobacter spp and Klebsiella spp. This together with the highly pathogenic Enterobacteriaceae including Salmonella spp and Shigella spp. Proteus spp, and Alklegens spp. Potential pathogenic organisms were also among the isolates. On the other hand Pseudomonas spp was isolated from 62% of randomly collected fishes. The number and percentages of the isolated bacteria were compared according to seasons. The total bacterial count, coliform count and E. coli count were estimated from all parts of collected samples. The negative impacts of the presence of Enterobacteriaceae and Pseudomonas spp in fishes were discussed based on their potential pathogenic effect toward public health and their role to enhance rapid spoilage of fishes.
Key words: Enterobacteriaceae, fisheries, public health, Pseudomonas.
Sanaa O. Yagoub