ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-005, April, 2019. © International Scholars Journals
Full Length Research Paper
Cytotoxicity and antimicrobial activity of Teucrium scordium L. (Lamiaceae) extracts
Tatjana Kundakovi 1*, Marina Milenkovi 2, Aleksandra Topi 3, Tatjana Stanojkovi 4, Zorica Jurani 4 and Branislava Lakuši 5
1Department of Pharmacognosy, Faculty of Pharmacy, 11000 Belgrade, Serbia.
2Department of Microbiology and Immunology, Faculty of Pharmacy, 11000 Belgrade, Serbia.
3Department of Medical Biochemistry, Faculty of Pharmacy, 11000 Belgrade, Serbia.
4Institute for Oncology and Radiology of Serbia, 11000 Belgrade, Serbia.
5Department of Botany, Faculty of Pharmacy, 11000 Belgrade, Serbia.
Accepted 03 April, 2019
Abstract
Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB- 361 cells (IC50=130.33±0.1 g/ml and IC50=189.89±3.99 g/ml, respectively). Dichlormethane extract was more effective against MDA-MB-453 cell line (IC50=130.33 ± 0.1 g/ml). The methanol extract of T. scordium possessed no cytotoxicity against breast cancer cell lines, MDA-MB-361 and MDA-MB-453. Herb extracts of T. scordium have shown weak antibacterial activity on Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis with no activity against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Enterococcus faecalis, and Candida albicans.
Key words: Teucrium scordium subsp. scordioides, cytotoxicity, antimicrobial activity.
Zorica Jurani and Branislava Lakuši, Tatjana Stanojkovi, Aleksandra Topi, Tatjana Kundakovi*, Marina Milenkovi
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-009, April, 2019. © International Scholars Journals
Full Length Research Paper
Optimized antibacterial measures against Escherichia coli O157:H7 and Staphylococcus aureus
Raftari, M.1, Azizi Jalilian F.2, Abdulamir, A. S.2,3, Sobhan Ghafurian2, Radu S.1, Sekawi Z.2, Abu Bakar F.1*
1Faculty of Food Science and Technology, Universiti Putra Malaysia, 43300, Serdang, Selangor, Malaysia.
2Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43300, Serdang, Selangor, Malaysia.
3Alnahrain Univerty, Faculty of Medicine, 14222, Iraq.
Accepted 15 January, 2019
Abstract
Varied mixtures of different concentrations 1, 1.5 and 2% of acetic (AA), lactic (LA), propionic (PA) and formic (FA) acids at 1:1 ratio were spray- washed on inoculated meat to evaluate their efficacy in reducing loads of Escherichia coli O157:H7 and Staphylococcus aureus on meat tissue. It was found that increasing the concentration of the used organic acids increased the bacterial lethality proportionally. And significant difference (P<0.05) was observed in the lethal effect of different mixtures and concentrations of the used organic acids. As a novel combination, FA treatments as combinations with AA, LA, and PA, especially FA with LA, reduced bacterial loads greatly, up to 3 logs cfu/ml and eradicated inoculated bacteria, E. coli O157:H7 and S. aureus completely within 3-6 days. This reduction was higher than that incurred by other combinations. Significantly, higher log reductions by the used organic acids were obtained for S. aureus than for E. coli O157:H7. It was concluded that the combination of LA and FA treatment was a highly promising, feasible, and economical method of decontaminating meat surface from both E. coli O157:H7 and S. aureus bacteria. Moreover, it is safe if compared with other approaches.
Key words: Antibacterial, Escherichia coli, Staphylococcus aureus, organic acid, sterilization, meat.
Radu S, Sekawi Z and Abu Bakar F*, M , Abdulamir , Azizi Jalilian F, Raftari , Sobhan Ghafurian, A. S
Page: 1 - 9
Review
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Review
Chromobacterium violaceum infection in China: Three case reports and literature reviews
Tai Ma1, Wei Shi3, Jun Cheng1, Jian-Kang Zhang1, Li-Fen Hu1, Ying Ye1,2 and Jia-Bin Li1,2*
1Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, 230022, Hefei, Anhui Province, China.
2Anhui Center for Surveillance of Bacterial Resistance, 230022, Hefei, Anhui Province, China.
3Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060; China.
Accepted 22 January, 2019
Abstract
Chromobacterium violaceum, Gram-negative Bacillus, is a common inhabitant of soil and stagnant water found in tropical and subtropical regions of the world. It is a rare cause of severe, often fatal, human disease. In the report, 3 cases of patients infected with C. violaceum were described in Anhui Province, China. Routine and bacteriological investigations were carried out to establish the aetiological diagnosis. Moreover, the patients were treated with appropriate antimicrobial agents and auxiliary therapy. To our knowledge, a total of 42 cases have been reported previously from Chinese mainland in the recent 20 years, with a review of the literatures.
Key words: Chromobacterium violaceum, infection, China.
Jian-Kang Zhang, Li-Fen Hu, Wei Shi, Ying Ye and Jia-Bin Li*, Tai Ma, Jun Cheng
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Assessing the potential of Tetradenia riparia in treatment of common skin conditions in rural communities of South Africa
R. M. Coopoosamy* and K. K. Naidoo
Department of Nature Conservation, Mangosuthu University of Technology, P. O. Box 12363, Jacobs, 4026, Durban, KwaZulu-Natal, South Africa.
Accepted 20 March, 2019
Abstract
Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in treatment of secondary infections, such as, mouth sores, pelvic or vaginal sores in individuals who are HIV/AIDS infected.
Key words: Tetradenia riparia, iboza, antimicrobial, traditional medicines.
R. M. Coopoosamy*, K. K. Naidoo
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII T30C
Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2
1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.
2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.
Accepted 03 March, 2019
Abstract
Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.
Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Molecular characterization of norovirus from acute gastroenteritis patients in Malaysia
H. Zuridah1,2*, Sufiyan Hadi M1, L. K. Teh2 and A. H. Zed Zakari1
1Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
2Pharmacogenomic Centre (PROMISE), Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
Accepted 11 February, 2019
Abstract
Rotavirus and recently norovirus have been described as important and most common cause of acute gastroenteritis in children. The mode of transmission is fecal-oral. Diagnosis of both of these viruses can be made by rapid antigen detection of the viruses in stool specimens and strains can be further characterized by enzyme immunoassay or reverse transcriptase polymerase chain reaction. Stool specimens collected from various hospitals in Malaysia were examined for norovirus by both immunochromatography and reverse-transcription polymerase chain reaction techniques. Rotavirus antigen was screened by a commercially available latex agglutination test kit. Altogether, 168 stool samples were collected for both norovirus and rotavirus screening. Out of these, 77/168 (45.8%) were examined for rotavirus with 17/77 (22%) rotavirus antigen positive. Due to very small amount of fecal materials obtained in some cases, only 151/168 (89.8%) were sufficient for norovirus and 14/151 (9.3%) were positive for norovirus genogroup II (GII). This study highlights that rotavirus remains the main agent for acute gastroenteritis and identification for emerging norovirus among the children is becoming important for proper patient management.
Key words: Norovirus, rotavirus, immunochromatography, latex agglutination, RT-PCR, phylogenetic analysis.
H. Zuridah*, Sufiyan Hadi M, L. K. Teh and A. H. Zed Zakari
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