ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Preparation and application of a novel environmentally friendly cucumber seed coating agent
Zeng Defang*, Tu Renjie and Wu Shan
School of Resource and Environmental Engineering, Wuhan University of Technology, Wuhan, People’s Republic of China.
Hubei Key Laboratory of Mineral Resources Processing and Environment, Wuhan 430070, People’s Republic of China.
Accepted 4 January, 2011
Abstract
A novel cucumber seed coating agent was made from natural polysaccharide, fertilizer and microelement, etc., which achieved a good effect on increasing cucumber yield and antifeeding against pests. The foremost difference between this seed coating agent and conventional ones was that it controlled pests through the approach of repelling pests and enhancing the immunity of seeds, but not by killing pests. Results indicated that this seed coating agent had excellent control of pests and diseases and increased yield by 8.4 to 10.8%, while the material cost was decreased by 16.7% compared with the conventional toxic seed coating agent. In addition, results showed it was safe for man and livestock without causing any pollution and harm. Therefore it had three characteristics of high yield, less cost and friendly environment.
Key words: Environmentally friendly type, cucumber seed coating agent, polysaccharide, antifeedant, germination percentage.
Zeng Defang*, Tu Renjie and Wu Shan
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Amylolytic enzymes produced by the fungus Colletotrichum gloeosporioides in rice semi-solid fermentation
Sideney Becker Onofre*, Paula Steilmnn, Julia Bertolini, Daniele Rotta, Aline Sartori, Francini Yumi Kagimura, Sara Ângela Groff and Luciana Mazzali
Laboratory of Microbiology of the Paranaense University – UNIPAR – Unit Campus Francisco Beltrão, Paraná, Brasil.
Accepted 08 March, 2011
Abstract
Amylases are enzymes that hydrolyze starch, releasing several products including dextrines and small polymers of glucose units. This work investigated the capacity of the endophytic fungus Colletotrichum gloeosporioides of producing the amylolytic enzymes a-amylase and glucoamylase through fermentation in semi-solid medium of residues of the processing of rice, without supplementation. The a-amylase activity was determined according to the methodology of Pandey et al. (2005) and that of glucoamylase was determined through the release of reducing sugars, dosed by the DNS method described by Soccol (1992). The results show that the fungus C. gloeosporioides showed the highest peaks of enzymatic production at 96 h, where 563.32 U/g of a-amylase and 345.30 U/g of glucoamylase were obtained at 28°C and pH 5.49. With these data it is concluded that the fungus C. gloeosporioides has great capacity of producing amylolytic enzymes (a-amylase and glucoamylase) through fermentation in rice-based solid state without supplementation.
Key words: Starch, biotechnology, enzymes, processes, endophytic,
Aline Sartori, Francini Yumi Kagimura, Julia Bertolini, Sara Ângela Groff and Luciana Mazzali, Daniele Rotta, Sideney Becker Onofre*, Paula Steilmnn
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Optimization of cellulase production by Aspergillus niger and Tricoderma viride using sugar cane waste
Ali A. Juwaied, Ahmed Abdulamier Hussain Al-amiery*, Zahraa Abdumuniem and Umar Anaam
Department of applied science, Biotechnology division, University of Technology, Baghdad, Iraq.
Accepted 1 February, 2011
Abstract
Sugar cane waste was used as substrates for the production of cellulase enzyme using Aspergillus niger and Tricoderm aviride. From the fermentation studies, sugar cane waste gave the best result with an enzyme activity value of (using A. niger and T. aviride) 2.7 and 2.6 IU/ml respectively. It gave the maximum enzyme activity at about the 10 days of cultivation, suggesting that is the optimum time when the enzyme may be harvested. The fermentation experiments were performed in solid stat fermentation (SSF). Incubation time and initial pH of fermentation medium watts optimized with simultaneous mixed culture. It was revealed that the sugar cane waste at pH = 4.5 and 40°C Aspergillus niger (37°C for T. viride) was the best source of carbon for the enhanced production of cellulase in the compatible culture experiments. Based on the reported results, it may be concluded that sugar cane waste can be a potential substrate for production of cellulase, incorporation of culturing A. niger or T. viride. The aim of this work is to produce cellulase from sugar cane waste and reduce the pollution.
Key words: Aspergillus niger, cellulase, culture, sugar cane waste.
Ali A. Juwaied, Ahmed Abdulamier Hussain Al-amiery*, Zahraa Abdumuniem and Umar Anaam
Page: 1 - 10
Short Communication
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Short Communication
Two new schifffnerulaceous fungi from Kerala, India
V. B. Hosagoudar1, J. Thomas1 and D. K. Agarwal2*
1Tropical Botanic Garden and Research Institute, Palode - 695 562, Thiruvananthapuram, Kerala, India.
2Plant pathology Division, IARI, New Delhi 110 012, India.
Accepted 27 April, 2011
Abstract
Two new species namely, SARCINELLA LORANTHACEARUM and SCHIFFNERULA MELIOSMATIS were collected from Peppara and Neyyar Wildlife sanctuaries and Silent Valley National park are described and illustrated in detail.
Key words: Black mildews, new species, Kerala, India.
V. B. Hosagoudar, J. Thomas and D. K. Agarwal*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Some new rust fungi (Uredinales) from Fairy Meadows, Northern Areas, Pakistan
N.S. Afshan1*, A. N. Khalid2 and A. R. Niazi2
1Centre for Undergraduate Studies, University of the Punjab Quaid-e-Azam Campus, Lahore, 54590, Pakistan.
2Department of Botany, University of the Punjab Quaid-e-Azam Campus, Lahore, 54590, Pakistan.
Accepted 9 September, 2012.
Abstract
Puccinia opizii on Carex curta is reported here as a new record for Pakistan. Furthermore, Coleosporium lycopi, Cronartium ribicola, Melampsora epitea, Peridermium thomsonii, Phragmidium rosae-moschatae, Puccinia carthami, Puccinia chrysanthemi, Puccinia circaeae, Puccinia graminis, Puccinia komarovii and Uromyces trifolii are additions to the rust flora of Fairy Meadows, Northern Areas, Pakistan.
Key words: Byal camp, carex curta, fairy meadows, Hunza, Nanga Parbat, Northern areas.
N.S. Afshan*, A. N. Khalid and A. R. Niazi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
A comparative study of ethanol production from various agro residues by using Saccharomyces cerevisiae and Candida albicans
Jayant Mishra1*, Deepesh Kumar2, Sumeru Samanta3 and Manoj Kumar Vishwakarma4
1Department of Biotechnology and Molecular Biology, Genetech Laboratories, Biotech Park, Lucknow, India.
2Saroj Institute of Technology and Management, Lucknow, India.
3Rohilikhand Medical College Bareily, India.
4Sagar Institute of Technology and management Barabanki, India.
Accepted 02 May, 2011
Abstract
In this study, ethanol produced from fruits of pineapple, orange and sweet lime was investigated. Effect of different constant times [24 and 72 h, and 8 days (in submerged fermentation)]; pH (3.5 to 8.5), temperature (78°C) and autoclave pre-treatment (121°C, 20min) were also studied to improve the yield of ethanol in fruits. Yeast (Saccharomyces cerevisiae and Candida alb cans) are used for fermentation. Fermentation process used solid state fermentation and submerged fermentation methods. The results showed that there is a substantial increase in the quantity of ethanol produced in submerged fermentation as compared to that produced by the solid state fermentation. Optimal ph and temperatures for the better yield of ethanol were 3.5 to 8.5 and 78°C respectively. Autoclave pre treatment protected the samples from contamination and increased the volume of ethanol.
Key word: Ethanol, fruits, sugar concentration, Saccharomyces crevasse and Candida albicans.
Jayant Mishra*, Sumeru Samanta and Manoj Kumar Vishwakarma, Deepesh Kumar
Page: 1 - 10