ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (1), pp. 001-005, January, 2020. © International Scholars Journals
Full Length Research Paper
Anti-Aspergillus properties of different extracts from selected plants
Ivana D. Radojević*, Milan S. Stanković, Olgica D. Stefanović, Marina D. Topuzović, Ljiljana R. Čomić and Aleksandar M. Ostojić
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
Accepted 19 September, 2019
Abstract
In vitro antifungal activity of methanol, acetone and ethyl-acetate extracts from Equisetum telmateia Ehrh., Allium flavum L., Sedum acre L., Sideritis montana L., Marubium peregrinum L. and Xeranthemum anuum L. grown in Serbia, were investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) have been determined. Testing was conducted against four Aspergillus species - A. flavus, A. fumigatus, A. niger and A. restrictus. All statistical analyses were performed using SPSS package. The tested extracts showed significant antifungal activity against A. restrictus and moderate activity against other Aspergillus spp. The best results showed ethyl acetate extract of S. montana, X. anuum and S. acre.
Key words: Antifungal activity, plant extracts, Aspergillus spp.
Ivana D. Radojević*, Olgica D. Stefanović, Ljiljana R. Čomić and Aleksandar M. Ostojić, Milan S. Stanković, Marina D. Topuzović
Page: 1 - 5
https://doi.org/10.46882/ajmr/1006Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (1), pp. 001-005, January, 2020. © International Scholars Journals
Full Length Research Paper
Comparative diagnosis of ovine brucellosis using single step blood-PCR with old and new serological tools
Salama M. Abdel Hafez1, Khaled A. Abd El-Razik2*, Hany M. Hassan1 and Ibrahim Gad3
1Immunology and immunobiology Unit- Animal Reproduction Research Institute-Haram-Giza-Egypt.
2Animal Reproduction Department, National Research Center, Dokki-Giza-Egypt.
3Infectious Diseases Department, Animal Reproduction Research Institute-Haram-Giza-Egypt.
Accepted 19 September, 2019
Abstract
Brucellosis is an important disease affecting mainly sheep and goats. Diagnosis based on isolation of Brucella organisms from the suspected animals is the golden standard but has a limited sensitivity, expensive and unpractical to apply on a large scale in control campaigns. Accordingly, the indirect diagnosis of disease based on serological tests is the method of choice in the eradication programs. In this study, a single step polymerase chain reaction (PCR) was used to diagnose brucellosis using sheep whole blood and compared its sensitivity and specificity against some of the most commonly used serological techniques and modified ones. Three hundred apparently healthy ewes were randomly chosen from different governorates of Egypt. Sera were tested against Rose Bengal test (RBT), Serum Agglutination test (SAT), ELISA using both the whole Brucella antigen (W-ELISA) and the periplasmic protein antigen (P-ELISA). Results showed that 39% of the blood samples were positive to the PCR test, Meanwhile 29.3, 27.0, 28.7 and 28.3% were positive to the previous serological tests respectively. We recommend the use of this blood PCR assay for accurate diagnosis of ovine brucellosis especially in the early stage of infection, which is difficult to achieve by the applied serological tests.
Key words: Ovine brucellosis, Blood PCR, RBT, SAT, ELISA.
Salama M. Abdel Hafez, Hany M. Hassan and Ibrahim Gad, Khaled A. Abd El-Razik*
Page: 1 - 5
https://doi.org/10.46882/ajmr/1004Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (1), pp. 001-008, January, 2020. © International Scholars Journals
Full Length Research Paper
Thermo-alkaliphilic halotolerant detergent compatible protease(s) of Bacillus tequilensis MTCC 9585
Imran Khan, Puja Gupta and Jyoti Vakhlu*
School of Biotechnology, University of Jammu, Jammu-180006, India.
Accepted, 19 September, 2019
Abstract
An extra-cellular thermo-alkaliphillic protease producing Bacillus was isolated from the soil and identified to be Bacillus tequilensis MTCC 9585 by microscopic, colony morphology, biochemical and 16S ribotyping. B. tequilensis MTCC 9585 produces protease up to 21 h of growth but interestingly 90% of the protease production occurred, just after 6 h of growth. The organism grew as well as and produced enzyme at wide pH (5 to 12) and temperature range (4, 25, 37 and 50°C), though optimum temperature and pH for the growth of the Bacillus were 37°C and pH 7.0. Optimum pH for enzyme activity coincided with optimum pH for enzyme production at pH 10. Optimum temperature for enzyme activity was 60°C and the enzyme stayed stable over the period of 270 days (9 months) at 10°C. Metal ions like Ca2+, Mg2+, K+ increased the enzyme activity whereas Cu2+, Zn 2+ inhibit the activity slightly. Wash performance and stain removal efficiency increased when partially purified enzyme was used in conjunction with selected detergent. B. tequilensis can be a potential candidate for use in detergent industry because: of couple of reasons such as (i) 90% of the protease is produced only after 6 h of growth (economically viable), (ii) it’s activity in wide pH and temperature range (iii) it’s stability over the period of 9 months at 10°C indicating good shelf life and (iv) detergent compatibility.
Key words: Bacillus tequilensis, alkaline protease, pH, temperature, detergent compatibility.
Puja Gupta and Jyoti Vakhlu*, Imran Khan
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (1), pp. 001-005, January, 2020. © International Scholars Journals
Full Length Research Paper
Metaproteomic evaluation on the microbial community functions in a mesoscale cyclonic eddy perturbation
Yong-Yu Zhang*, Zheng-hao Chen, Zhuo Zhen and Jun Yang
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Accepted 23 June, 2019
Abstract
Eddy pumping is a process by which mesoscale eddies induce isopycnal displacements that lift nutrient-replete waters into the euphotic zone, driving a set of biogeochemical processes in the ocean. To investigate the potential effect of physical processes on the microbial community functions, the microbial community metaproteomic profiles were determined in a cold-core cyclonic eddy in the South China Sea (SCS). The significant differences of metaproteomic patterns accurately predicted their distinct functional responses to environmental changes caused by the mesoscale cyclonic eddy perturbations. From the protein gel profiles, 11 proteins were successfully identified by MALDI TOF MS/MS, including nitrogen regulatory proteins, ribosomal proteins and substrate transport proteins, etc, which implied the metabolic responses of the microbial populations to the nutrients change within the cyclonic eddies. These results highlight the potential of metaproteomics for studying complex microbial consortium in the ocean and contributed to our understanding of the biological consequences of a mesoscale cyclonic eddy in the SCS.
Key words: Cyclonic eddy, metaproteomics, microbial community.
Zheng-hao Chen, Yong-Yu Zhang*, Zhuo Zhen and Jun Yang
Page: 1 - 5
https://doi.org/10.46882/ajmr/1002Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (1), pp. 001-004, January, 2020. © International Scholars Journals
Full Length Research Paper
Effects of lactic acid, fumaric acid and chlorine dioxide on shelf-life of broiler wings during storage
Canan HECER and Metin GULDAS*
Uludag University, Karacabey Vocational School, Department of Food Technology, 16700, Karacabey, Bursa Turkey.
Accepted 19 September, 2019
Abstract
In this study, effects of chlorine dioxide (0.3 and 0.5%), lactic acid (0.5 and 1.0%) and fumaric acid (0.5 and 1.0%) on the shelf-life of broiler wings were investigated. The samples were dipped into the experimental solutions for 10 min. and stored at +4°C for 9 days. Microbiologically, the counts of total aerobic mesophilic bacteria (TAMB), total psychrophilic bacteria (TPB) and ESCHERICHIA COLI were determined and pH values were measured. Compared to the control samples, the TAMB counts were reduced approximately as 13% with chlorine dioxide, 13.5% with lactic acid and 10.5% with fumaric acid. The most effective bacterial reduction on E. COLI was observed in the samples treated with 1.0% of lactic acid as 56 and 1.0% of fumaric acid as 34%. Statistically, the changes determined in the TPB counts were not significant (P>0.05), however they increased as 6 and 7% during the storage. Throughout the storage, the bacterial growth was reduced parallel to the concentration of the chemicals increased. The shelf-life of broiler wings was prolonged 4 days by the experimental solutions. Neither off-flavor nor a negative effect was observed on the sensorial properties by the panelists due to chemical applications.
Key words: Broiler, chlorine dioxide, lactic acid, fumaric acid, antimicrobials.
Metin GULDAS*, Canan HECER
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-008, June, 2019. © International Scholars Journals
Full Length Research Paper
Density functional theory (DFT) study of C7 Si5 Ge3 cluster as a novel material for vitamin C nano carrier
M. Monajjemi1*, T. Ardalan2, H. Seyed Hosseini Ghaheh2 and F. Mollaamin3
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Science and Research Branch, Islamic Azad University, Tehran, Iran.
3Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran.
Accepted 23 April, 2019
Abstract
In the present study, structural properties of C7 Si5 Ge3 cluster and interaction between this cluster and vitamin C have been studied extensively utilizing density functional theory (DFT) employing B3LYP exchange correlation. Nuclear magnetic resonance (NMR) properties are calculated by using density functional method (B3LYP) with 6-31G, 6-311G* and cc-pvdz as basis sets. Also natural bond orbital (NBO) analysis has been performed for C7 Si5 Ge3 cluster and C7 Si5 Ge3 inside vitamin C. Our results indicate that vitamin C can form stable bindings with C7 Si5 Ge3 cluster through oxygen (O) active site. Thus, we arrive at the prediction that the C7 Si5 Ge3 cluster can be implemented as a novel material for drug delivery applications.
Key words: C7 Si5 Ge3, vitamin, nano, nuclear magnetic resonance, natural bond orbital, density functional theory.
T. Ardalan, M. Monajjemi*, H. Seyed Hosseini Ghaheh and F. Mollaamin
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