ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Prevalence of intestinal parasites at Ujjain, Madhya Pradesh, India: Five-year study
Yogyata Marothi* and Binita Singh
Department of Microbiology, R.D.Gardi Medical College, Ujjain (MP), Madhya Pradesh, India.
Accepted 13 February, 2019
Abstract
This study was conducted to determine the prevalence of intestinal parasites in hospitals at Ujjain. A total of 5990 samples were collected in five years: 3580 from Ujjain Charitable Trust Hospital (Urban population) and 2410 from R. D. Gardi Medical College (Rural population). Overall prevalence rate of intestinal parasite was 21.4%. Entamoeba histolytica (10.5%) was the commonest protozoa followed by Giardia lamblia (3.9%). Among the helminths, Ascaris lumbricoides (2.8%) was the commonest. Multiple infections were seen in 70 samples. There was difference in prevalence between urban (20.2%) and rural (23.1%) population. Females (27.4%) were more affected than males (18.2%) and age group 0 to 10 years old had the highest rate of infection. The results indicate that there is a need to implement control measures in form of regular deworming, health education and provision of safe water supply.
Key words: Intestinal parasites, prevalence, rural, urban, Ujjain.
Binita Singh, Yogyata Marothi*
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-009, April, 2019. © International Scholars Journals
Full Length Research Paper
Determining the knowledge of food safety and purchasing behavior of the consumers living in Turkey and Kazakhstan
Nevin Sanlier1*, Ayhan Dağdeviren, Bülent Çelik, Saniye Bilici and Aktolkin Abubakirova
Department of Nutrition and Dietetics, Faculty of Health Science, Gazi University, Ankara, Turkey.
Accepted 18 February, 2019
Abstract
Complete and balanced nutrition with reliable food consists of the basis of health and protective health services. Therefore, the current study was carried out to determine the knowledge of food safety level and purchasing behavior of 668 consumers living both in Turkey (n=348) and in Kazakhstan (n=320) and to compare the results. Volunteered consumers for the research were given a face to face interview between March and September 2010. It was found that the knowledge of purchasing behavior (14.43±2.56) of food safety (20.82±4.20) of the consumers living in Turkey was higher compared to those living in Kazakhstan (11.84±2.92, 14.74±3.86 respectively) and that the difference between the two countries was statistically significant (p<0.01). In addition, a positive correlation was found between knowledge of food safety and purchasing behavior (r=0.541, p< 0.01); age and purchasing behavior (r=0.325, p< 0.01) and knowledge score of food safety (r=0.148, p< 0.01).
Key words: Purchasing behavior, knowledge of food safety, consumer, Turkey, Kazakhstan.
Saniye Bilici and Aktolkin Abubakirova, Nevin Sanlier*, Ayhan Dağdeviren, Bülent Çelik
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
Theoretical studies on nanostructure of 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde
M. Monajjemi1*, F. Naderi2, F. Jadidi3 and F. Mollaamin4
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of basic science, Shahr e Qods branch, Islamic Azad University, Iran.
3Science and Research Branch, Islamic Azad University, Tehran, Iran.
4Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Iran.
Accepted 15 March, 2019
Abstract
The polarized continuum model (PCM) model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in solutions. The maximum IR intensity is related to the C4=C5 stretching vibrations. Also, the vibrational free energy, heat capacity, entropy, thermal energy and zero point vibrational energy obtained from the calculated frequencies and these are compared in different media.
Key words: Polarized continuum model (PCM), functional theory methods (DFT), solvent, IR, safranal.
F. Jadidi and F. Mollaamin, F. Naderi, M. Monajjemi*
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Ultrasound-targeted microbubble destruction promotes proliferative vitreoretinopathy induced by RPE-J cells and platelet-rich plasma
Xiaozhi Zheng1, Ping Ji1 and Jianqun Hu2*
1Department of Ultrasound, The Fourth Affiliated Hospital of Nantong University (The First People’s Hospital of Yancheng), Yancheng 224006, Jiangsu Province, P. R. China.
2Department of Ultrasonic Diagnosis, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing 210029, Jiangsu Province, P. R. China.
Accepted 23 January, 2019
Abstract
Ultrasound-targeted microbubble destruction (UTMD) has been considered as a new approach to the gene therapy of proliferative vitreoretinopathy (PVR), but whether it affect the in vivo PVR model induction remains unknown. In this study, 90 Wistar rats were averagely divided into three groups according to the content of intravitreal injection: normal saline (Group 1), retinal pigmented epithelial -J cells and PRP (Groups 2 and 3). In Group 3, a condition of UTMD was used additionally on days 3 and 7 after injection. On days 14 and 28, the pathological changes of eye grounds were assessed, and the expression levels of transforming growth factor (TGF)- 2 and platelet -derived growth factor (PDGF)-BB were tested. In Group 3, proliferation in the eyes was significantly stronger and faster than that of Group 2 and the ratio of PVR was significantly higher than that of Group 2. The expression levels of TGF- 2 and PDGF-BB were significantly higher in Group 3 than in Group 2. These data suggested that UTMD promotes PVR induced by RPE- J cells and PRP which provide a new method for the development of rat PVR model. This also reminds us that the effects of UTMD should be taken into consideration when using UTMD as an approach to attenuate PVR.
Key words: Proliferative vitreoretinopathy, rat model, ultrasound-targeted microbubble destruction, retinal pigmented epithelial -J cells, platelet-rich plasma.
Ping Ji and Jianqun Hu*, Xiaozhi Zheng
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-009, March, 2019. © International Scholars Journals
Full Length Research Paper
Optimization of growth conditions for mycelial yield and exopolysaccharride production by Pleurotus ostreatus cultivated in Nigeria
Adebayo-Tayo B. C.1, Jonathan S. G.1*, Popoola O. O.1 and Egbomuche R. C.2
1Department of Botany and Microbiology, University of Ibadan, Oyo state, Nigeria.
2Department of Microbiology, University of Uyo, Akwa Ibom State, Nigeria.
Accepted 11 January, 2019
Abstract
Optimum culture condition for mycelia and exopolysaccharides production (EPS) by Pleurotus ostreatus in submerged liquid culture was investigated. The optimum temperature for mycelia yield and exopolysaccharide production was 25 and 15°C in day 14 with yields of 1.40 g/ml and 2700 mg/l respectively. Optimum pH was found to be 8 with respective yields of 2.5 g/ml and 2665 mg/l in day 12 for mycelia and EPS mannitol (9.75 g/litre) and raffinose stimulated the highest mycelia growth (3.0 g/ml) and EPS production (3000 mg/l) at day 14 respectively. Mycelia yield (2.10 g/ml) and EPS production (2700 mg/l) was optimally supported by urea. Glycine (1.0 g) and leucine (1.0 g) supported optimum production of mycelia yield (2.5 g/ml) and EPS concentration (2925 mg/l) respectively while folic acid and ascorbic acid induced the moderate mycelia yield and EPS concentration respectively. These results have shown that significant improvement in mycelia yield and EPS production by P. ostreatus could be enhanced through submerged cultivation under appropriate optimized conditions.
Key words: Culture, Pleurotus ostreatus, mycelia yield, exopolysaccharride-production.
Adebayo-Tayo B. C, Jonathan S. G*, Popoola O. O and Egbomuche R. C
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-008, March, 2019. © International Scholars Journals
Full Length Research Paper
Comparison of the electro transformation of plasmids and plasmid stability between Zymomonas mobilis ZM4 and CP4
Shaolan Zou1*, Minhua Zhang1,2, Jiefang Hong1, Yuanyuan Ma1 and Weina Zhang1
1Biomass Conversion Laboratory, Tianjin R&D Center For Petrochemical Technology, Tianjin University, Tianjin, 300072, China.
2State Key Laboratory of Engine, Tianjin University, Tianjin, 300072, China.
Accepted 11 January, 2019
Abstract
Four different plasmids were electro transformed into Zymomonas mobilis ZM4 and CP4, two important ethanol-producing strains. The results showed that the best source strain for preparing plasmids was the transformed host strain itself, and Escherichia coli JM110 as the source strain could yield significantly higher transformation efficiencies than Top10. The optimal recovery time of transformed ZM4 or CP4 cells to obtain maximum number of transformants and highest transformation efficiency was 11 h for pZB21-mini, pZB21 and pZA22, but 24 or 20 h for pBBR1MCS-2. The optimal electric field strength for pZB21-mini was 13.25 kV /cm in ZM4 and 14.0 kV /cm in CP4.But for pZA22 and pBBR1MCS-2, it was 11.75 kV /cm in ZM4 and 12.5 kV /cm in CP4; for pZB21, also 12.5 kV /cm in CP4.These plasmids were shown to be more stable in ZM4 than in CP4 by serial transfer to antibiotic-free medium and the 3 plasmids were more stable than pBBR1MCS-2. The results will help to support the genetic and biotechnological research of Z. mobilis by providing information about some of the most important factors that influence the transformation of ZM4 and CP4, and also providing insights into the similarities and differences in their restriction-modification (R-M) systems.
Key words: Zymomonas mobilis, plasmids, electroporation, transformation, plasmid stability.
Minhua Zhang, Shaolan Zou*, Yuanyuan Ma and Weina Zhang, Jiefang Hong
Page: 1 - 8