ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (5), pp. 001-004, May, 2018. © International Scholars Journals
Full Length Research Paper
The effect of short-time microwave exposures on Escherichia coli O157:H7 inoculated onto beef slices
A. Jamshidi1*, H. A. Seifi2 and M. Kooshan3
1Department of food hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran.
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran.
3DVM degree, Graduated from School of Veterinary Medicine, Ferdowsi University of Mashhad, Iran.
Accepted 22 January, 2018
Abstract
Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, hemolytic-ureamic syndrom and thrombotic thrombocytopenic purpura. In this study the effect of microwave irradiation of beef samples which were inoculated with E. coli O157:H7 were investigated. The portions of fresh beef slices weighting 200 g each and about 10 × 10 × 2 cm in size, were soaked in fully growth of E. coli O157:H7, in BHI broth. The swab samples were taken from the contaminated samples, after different times of radiation (10, 20, 30, 40 and 50 s), using a domestic microwave oven at full power. The bacterial counts were performed by using surface plating on sorbitol Mac Conkey agar supplemented with cifixime and potassium tellurite. After each experiment the surface temperature of treated samples were measured. The experiment was carried out in triplicate and it was concluded that the microwave radiation which enhance the surface temperature more than 70°C, can eliminate the superficial contamination of cattle beef slices with E. coli O157:H7.
Key words: Escherichia coli O157:H7, microwave, beef.
A. Jamshidi*, H. A. Seifi and M. Kooshan
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (5), pp. 001-008, May, 2018. © International Scholars Journals
Full Length Research Paper
An inquiry of knowledge, attitudes and practices against pandemic H1N1 influenza among Turkish health care workers: Experience of a single center in Southeast of Turkey
Selda Aslan1*, Serda Gulsun1, Elvan Caglar Citak2, Ahsen Oncul1 and Habibe Pirinccioglu1
1Department of Infectious Disease, Diyarbakir State Hospital, Diyarbakir, Turkey.
2Department of Pediatric Oncology, Faculty of Medicine, Mersin University, Mersin, Turkey.
Accepted 25 January, 2018
Abstract
It is crucial to have a knowledge of pandemia. The aim of this study was to assess knowledge, attitude and behavioral responses of health care workers (HCWs) toward pandemic H1N1 influenza (PI). A cross-sectional study was performed in September 2009 in Diyarbakir, Turkey. All HCWs replied to a self-administered standardized, structured questionnaire. A total of 783 participated, with 31.55% having low knowledge and 22.98% having high level about PI. It was observed that HCWs sex was affecting knowledge level scores (p = 0.005). 26.44% had low knowledge about nosocomial preventive cautions, with 24.90% having high level of knowledge. The significant predictor for higher knowledge scores about preventive measures for nosocomial transmission was HCWs occupational status (p < 0.001). The study therefore reveals that, nosocomial transmission is a major problem during a pandemic and HCWs will be essential for effective working of the health system. Efforts should be targeted at educating HCWs to improve knowledge, attitude and behavioral responses in the current pandemia, as well as for future epidemics.
Key words: Pandemic H1N1 influenza, health care worker, nosocomial transmission, hospital.
Selda Aslan*, Ahsen Oncul and Habibe Pirinccioglu, Elvan Caglar Citak, Serda Gulsun
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (5), pp. 001-006, May, 2018. © International Scholars Journals
Full Length Research Paper
Antimicrobial activity of ten common herbs, commonly known as ‘Dashapushpam’ from Kerala, India
Vijayan Mini N.*, Barreto Ida, Dessai Seema, Dhuri Shital, D’ Silva Riva and Rodrigues Astrida
Department of Botany, Carmel College, Nuvem, Goa- 403604, India.
Accepted 08 February, 2018
Abstract
Ten herbs which are widely used in Ayurvedic system of medicine and are collectively known as “Dashapushpam “in Kerala, (India), were screened for their antimicrobial properties against nine spp. of pathogenic fungi and seven spp. of pathogenic bacteria. In the preparation of extracts, the entire shoot systems were used for Cardiospermum halicacabum and Evolvulus alsinoides and only leaves for others. Crude plant extracts were prepared by cold extraction with acetone. Two sets of pathogenic fungi-Set-1 and Set- 2-were used with Nystatin and Amphotericin as standards, respectively. From Set-1, all the extracts showed antimicrobial properties at least with two fungal species, the most commendable being the extract of Vernonia cinerea which was effective against all the fungal strains, outscoring the standard Nystatin. Only Ipomoea sepiaria and V. cinerea could inhibit the growth of Rhodotorula sp. Among the six extracts tested with Set- 2, only I. sepiaria and V. cinerea were effective which inhibited the growth of only one strain, in sharp contrast to the activity of the standard, Amphotericin which was highly effective against all other spp. The extracts of Aerva lanata, C. halicacabum and V. cinerea inhibited the growth of six, seven and four species of bacteria respectively. Results show that the herbal extracts involved are more effective against pathogenic fungi than pathogenic bacteria and throws light on the future prospects of plants as sources of potent antibiotics.
Key words: Antimicrobial, pathogenic fungi, pathogenic bacteria, crude plant extract, dashapushpam.
Dhuri Shital, D’ Silva Riva and Rodrigues Astrida, Barreto Ida, Dessai Seema, Vijayan Mini N.*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (5), pp. 001-007, May, 2018. © International Scholars Journals
Full Length Research Paper
Cultivation of an edible desert truffle (TERFEZIA BOUDIERI Chatin)
A. Slama1*, Z. Fortas2, A. Boudabous3 and M. Neffati1
1Range Ecology Laboratory, Institut des Régions arides de Médenine 4119, Tunisia.
2Laboratory of Microbiology, Faculty of Sciences Orans Es-Sénia, Algeria.
3Laboratory of the Microbiology and Actives Biomoleculs, Faculty of Sciences of Tunis, campus 1060, Tunisia.
Accepted 18 February, 2018
Abstract
This research was conducted to study the fruiting of an edible truffle TERFEZIA BOUDIERI Chatin in fields using mycorrhizal plants of HELIANTHEMUM SESSILIFLORUM Desf. Pers. in two soil types (gypsy and sandy loam soils). Two cultivation methods have been tested. The first is the transplantation of inoculated plants (with T. BOUDIERI ascospores) maintained in greenhouse and the second is the seedling of inoculated H. SESSILIFLORUM seeds in experimental field (24 m2). The first ascocarps was detected one year after (2006) nearby transplanted plants in gypsy soil, two fruiting bodies of T. BOUDIERI were observed in both soil types near the plants obtained by direct inoculation of H. SESSILIFLORUM seeds in the second year (2007). Three years after (2009), the experimental field produces two ascocarps close to transplanted plants in sand loamy soil and directly seedling plants in gypsy soil.
Key words: Helianthemum sessiliflorum, arid land, desert truffle, cultivation, plantation, mycorrhization, seeds.
A. Slama*, A. Boudabous and M. Neffati, Z. Fortas
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (4), pp. 001-007, April, 2018. © International Scholars Journals
Full Length Research Paper
Microbial flora on the hands of healthcare workers
Fahriye Eksi*, Aysen Bayram, Murat Mehli, Sadik Akgun and Iclal Balci
Gaziantep University, Faculty of Medicine, Department of Medical Microbiology, Universite Bulvari, Gaziantep, 27310, Turkey.
Accepted 08 January, 2018
Abstract
The aims of this study were to study the process of microbial contamination of healthcare workers’ (HCWs) hands during routine patient care and simultaneously to evaluate the state of antibiotic susceptibility of microorganisms isolated from the hands of HCWs. Samples were collected from the hands of 154 HCWs working at Gaziantep University Hospital. A standard bag broth technique was employed. In addition to conventional methods, VITEK 2 automated system and API ID 32C kits were used for identification of the isolated microorganisms. Antimicrobial susceptibility of bacterial agents was performed by disc diffusion method. For antifungal susceptibility, ATB Fungus 2 kit was used. Of 154 specimens, 148 (96.1%) showed microbial growth and 160 microorganisms were isolated as single or multiple members of the resident flora. Transient flora including one or more microorganisms (n = 47) were recovered from the hands of 39 (25.3%) HCWs. All gram-positive bacteria were detected to be sensitive to vancomycin, teicoplanin and linezolid, however 30.1% of the coagulase-negative staphylococci and 40% of Staphylococcus aureus isolates were resistant to methicillin. Multiple drug resistance was determined in Acinetobacter and Pseudomonas isolates. It can be concluded that transient flora members isolated from hands of HCWs can be causative agents of serious nosocomial infections due to their high and multiple antimicrobial resistance patterns.
Key words: Healthcare workers, resident flora, transient flora, antimicrobial susceptibility.
Fahriye Eksi*, Murat Mehli, Sadik Akgun and Iclal Balci, Aysen Bayram
Page: 1 - 7
Short Communication
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (4), pp. 001-003, April, 2018. © International Scholars Journals
Short Communication
Coxiella burnetii in goat bulk milk samples in Iran
Ebrahim Rahimi
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. E-mail: [email protected]
Accepted 19 March, 2018
Abstract
Q fever is a zoonotic disease caused by the rickettsial organism Coxiella burnetii, the objective of this study was to determine the prevalence rate of C. burnetii in bulk milk samples from dairy goat herds in Fars, Ghom, Kerman, Khuzestan and Yazd provinces, Iran. In the present study, 296 bulk milk samples from 89 dairy goat herds were tested for C. burnetii using a nested PCR assay. The animals which their milk samples collected for this study were clinically healthy. In total, 6 of 296 (2.0%) goat milk samples were positive; the positive samples originated from 4 of 22 (18.2%) dairy herds in Fars, 1 of 24 (4.2%) dairy herds in Khuzestan and 1 of 18 (5.5%) dairy herds in Yazd. All 76 goat bulk milk samples from 25 goat breeding farms collected in Ghom and Kerman provinces were negative, although no extensive prevalence study was undertaken, the results of this study indicate those clinically healthy dairy goats are important sources of C. burnetii infection in Iran.
Key words: Coxiella burnetii, PCR, milk, goat, Iran.
Ebrahim Rahimi
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