ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-005, April, 2019. © International Scholars Journals
Full Length Research Paper
Anti-cyclic citrullinated peptide antibodies in rheumatoid arthritis, brucellar arthritis and Behcet’s disease
Buket Cicio lu Arido an1*, Emel Sesli Çetin1, Mehmet Yildirim2, Selçuk Kaya1, Ali Murat Ceyhan2, Mustafa Demirci1, Serpil Sava3, Onur Ural4 and Selami Akku3
1Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
2Department of Dermatology, Faculty of Medicine, Suleyman Demirel University, Turkey.
3Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Katip Çelebi University zmir, Turkey.
4Department of Physical Therapy and Rehabilitation, Faculty of Medicine, Suleyman Demirel University Isparta, Turkey.
5Department of Infectious diseases, Faculty of Medicine, Selçuklu, Selcuk University, Turkey.
6Department of Physical Therapy and Rehabilitation, Faculty of Medicine, Yıldırım Beyazıt University Ankara, Turkey.
Accepted 23 March, 2019
Abstract
In Behcet’s disease (BD) and Brucellosis, joint involvement is very common and it may share similarities with rheumatoid arthritis (RA). Anti-cyclic citrullinated peptides (CCP) are evaluated as more specific autoantibodies than rheumatoid factor (RF) in RA. The aim of this study is to determine whether anti-CCP antibodies are useful in the differential diagnosis of RA from other arthritis or not. 153 serum samples were used in the study; 34 from RA patients, 26 from brucellar arthritis, 20 from BD with arthritis, 41 from BD without arthritis and 32 from healthy controls. In vitro quantitative, anti-CCP levels were measured by using fluoroenzyme-immunoassay (FEIA) method. Among RA patients, 73.5% were anti-CCP-positive and 70.5% were RF-positive. While there was a statistical difference in the anti- CCP and RF positivity rates between RA and brucellar arthritis patients, BD patients and healthy controls (p = 0.0001), there was no statistical difference between BD patients with arthritis and BD patients without arthritis. Also none of the Brucellar arthritis patients was positive for anti-CCP antibodies, one of them were positive for RF. Our results indicate that anti- CCP antibodies are not associated with brucellar arthritis or BD with joint involvement and since they show high specificity and good sensitivity for RA, they can be evaluated as a better marker than RF especially for the differentiation of RA from other types of arthritis such as BD and brucellar arthritis.
Key words: Rheumatoid arthritis, Behcet's disease, brucellar arthritis, anti-cyclic citrullinated peptide antibody, rheumatoid factor.
Buket Cicio lu Arido an*, Emel Sesli Çetin, Mehmet Yildirim, Serpil Sava, Ali Murat Ceyhan, Onur Ural and Selami Akku, Selçuk Kaya, Mustafa Demirci
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Assessing the potential of Tetradenia riparia in treatment of common skin conditions in rural communities of South Africa
R. M. Coopoosamy* and K. K. Naidoo
Department of Nature Conservation, Mangosuthu University of Technology, P. O. Box 12363, Jacobs, 4026, Durban, KwaZulu-Natal, South Africa.
Accepted 20 March, 2019
Abstract
Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and fungal strains further indicated the high value of this plant species in the medicinal world and could aid in treatment of secondary infections, such as, mouth sores, pelvic or vaginal sores in individuals who are HIV/AIDS infected.
Key words: Tetradenia riparia, iboza, antimicrobial, traditional medicines.
R. M. Coopoosamy*, K. K. Naidoo
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-007, April, 2019. © International Scholars Journals
Full Length Research Paper
A laboratory scale bioremediation of Tapis crude oil contaminated soil by bioaugmentation of ACINETOBACTER BAUMANNII T30C
Lee Kok Chang1*, Darah Ibrahim1 and Ibrahim Che Omar2
1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang Malaysia.
2Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Karung Berkunci 36, 16100 Pengkalan Chepa, Kelantan, Malaysia.
Accepted 03 March, 2019
Abstract
Microbial degradation of Tapis crude oil contaminated soil by ACINETOBACTER BAUMANNII T30C was conducted to evaluate the efficiency of the selected potential hydrocarbon degrader in stimulating bioremediation of crude oil-contaminated soil with different treatment units in microcosms with 2.5 kg soil. Previously, A. BAUMANNII T30C was isolated from a Tapis crude oil-contaminated soil of oil refinery plant, Terengganu, Malaysia. The reduction of residual hydrocarbons in the soil was observed for a period of about 35 days. The study showed that amendment of nutrients was needed for stimulating the growth of A. BAUMANNII T30C and indigenous microorganism in assisting the degradation of residual hydrocarbons in the soil.
Key words: Bioremediation, bioaugmentation, aliphatic hydrocarbons, amendment of nutrients.
Lee Kok Chang*, Darah Ibrahim and Ibrahim Che Omar
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-008, April, 2019. © International Scholars Journals
Full Length Research Paper
A novel poly (L-lactide) degrading thermophilic actinomycetes, Actinomadura keratinilytica strain T16-1 and pla sequencing
Sukhumaporn Sukkhum1,5, Shinji Tokuyama2, Prachumporn Kongsaeree3, Tomohiko Tamura4, Yuumi Ishida4 and Vichien Kitpreechavanich1,6*
1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
2Department of Biological chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
3Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
4Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, Kisarazu 292-0818, Japan.
5Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
6Center for Advanced Studies in Tropical Natural Resources, Institute for Advanced Studies and Graduated School, Kasetsart University Bangkok 10900, Thailand.
Accepted 15 February, 2019
Abstract
An actinomycete strain T16-1 demonstrated the maximum poly (L-Lactide)-degrading activity when cultured in basal liquid medium at 50°C. According to 16S rDNA sequence analysis, chemotaxonomic and DNA-DNA hybridization revealed that strain T16-1 belong to the family Thermomonosporaceae, genus Actinomadura. On the basis of phenotypic and phylogenic data, strain T16-1 which is a novel PLA-degrading thermophilic actinomycete was identified as Actinomadura keratinilytica, but the color of its colony on ISP plates, NaCl tolerant and utilization of mannitol, raffinose and arabinose were different. Partial sequence of poly (L-lactide) depolymerase gene from strain T16-1 was demonstrated. The gene consisting of 222 amino acids was related to serine protease from Streptomyces sp. with 43 to 46% identity.
Key words: Actinomadura keratinilytica, cloning, identification, poly (L-lactide).
Shinji Tokuyama, Yuumi Ishida and Vichien Kitpreechavanich*, Sukhumaporn Sukkhum, Prachumporn Kongsaeree, Tomohiko Tamura
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-003, April, 2019. © International Scholars Journals
Full Length Research Paper
Antibacterial activity of plant extracts against oral and skin pathogens
Majid Alipour* and Omid Khanmohammadi
Department of microbiology, Islamic Azad University (IAU) - Babol Branch, Babol, Iran.
Accepted 23 March, 2019
Abstract
The aim of this study is to examine the antibacterial activity of plant extracts. Both broth dilution and disc diffusion methods were used to assess the antibacterial activity of these extracts against skin and oral pathogens. Eryngium caucaseum Trautv, Eryngium bungei Boiss and Adiantum capillus-veneris have shown antibacterial activity against Streptococcus pyogenes, Streptococcus mutans, Staphylococcus aureus and Streptococcus sanguis. E. caucaseum Trautv showed the highest inhibition zone (> 30 mm) against S. pyogenes. The growth of S. pyogenes was remarkably inhibited by the ethanolic extract of the three plant extracts.
Key words: Adiantum capilis–veneris, Eryngium bungei Boiss, plant extracts.
Majid Alipour*, Omid Khanmohammadi
Page: 1 - 3
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-006, April, 2019. © International Scholars Journals
Full Length Research Paper
Quinolone resistance in Escherichia coli and Salmonella spp. isolates from diseased chickens during 1993-2008 in China
Xiang Chen1,2, Weijuan Pan1, Weiqiu Zhang1, Zhiming Pan1, Song Gao1 and Xinan Jiao1,2*
1Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Jiangsu 225009, China.
2Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Jiangsu 225009, China.
Accepted 23 March, 2019
Abstract
A total of 363 Escherichia coli and 224 Salmonella spp. were isolated from diseased chickens during 1993-2008 in China. The susceptibility to eight quinolones and prevalence of plasmid-mediated quinolone resistance (PMQR) determinants was investigated in these isolates. Among the E. coli isolates obtained during 1993-1999, 65.2% were resistant to nalidixic acid, while more than 50% of the E. coli isolates collected during 2000-2008 were resistant to 7 quinolones. All 101 Salmonella spp. isolates obtained during 1993-1999 were susceptible to quinolones, while more than 50% of the Salmonella spp. isolates collected during 2000- 2008 were resistant to only nalidixic acid (82.9%) . Among the 363 E. coli isolates, 4 (1.1%) were positive for aac(6 )-Ib-cr, 3 (0.8%) for qepA and 1 (0.3%) for qnrB10. No PMQR gene was identified in 224 Salmonella spp. isolates. The resistance of E. coli and Salmonella spp. to quinolones has been increasing in the past twenty years and the resistance of Salmonella spp. was much lower than that of E. coli, although they were separated in the same period. There is a rising trend of avian isolates harboring PMQR genes in China.
Key words: Quinolone resistance, qnr; aac(6 )-Ib-cr; qepA, chicken.
Xiang Chen, Zhiming Pan, Song Gao and Xinan Jiao*, Weijuan Pan, Weiqiu Zhang
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