ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-005, June, 2018. © International Scholars Journals
Full Length Research Paper
Physico-chemical and microbiological properties of honey samples obtained from Ibadan
M. O. Adenekan1, N. A. Amusa2*, A. O. Lawal2 and V. E. Okpeze1
1Federal College of Agriculture, IAR&T, P. M. B. 5029 Ibadan, Oyo State, Nigeria.
2Department of Plant Science and Applied Zoology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
Accepted 23 April, 2012
Abstract
This research paper investigated the physico -chemical and microbiological properties of 10 samples of honey obtained from Ibadan, Oyo State. The physicochemical properties evaluated were colour, water activity, pH, moisture content, ash content and electrical conductivity, while the standard plate count (SPC), total coliforms, Bacillus spp., yeast and mould fungi were the microbiological properties evaluated. Results showed a range of honey colours from light amber to dark amber. There was significant difference in the pH of the honey samples obtained from different areas of Ibadan. Minimum pH of 2.80 was observed from honey sample collected from Idi-Ayunre, while the highest pH value of 4.50 was from honey collected from Iwo Road. Total acidity value obtained ranged from 24.60 to 41.20 meq kg -1, while moisture content was 18.30%, ash content 0.50 g 100 g-1 and the electrical conductivity of 0.64 mScm-1. Results of the microbiological characteristics showed that the microbial profile were very low for all the microorganisms studied. The SPC varied from 0 - 200 cfu g-1 whereas total coliform were not detected in any of the samples and fungi (yeast and moulds) were also present at low counts in all the honey samples obtained from Ibadan.
Key words: Natural honey, physico-chemical properties, microbiology, Ibadan.
M. O. Adenekan, A. O. Lawal and V. E. Okpeze, N. A. Amusa*
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-007, June, 2018. © International Scholars Journals
Full Length Research Paper
BACILLUS LICHENIFORMIS IITRHR2: A novel source of antimicrobial proteinaceous food substance
Sapna Sharma1, Ram L. Singh2 and Poonam Kakkar1*
1Herbal Research Section, Indian Institute of Toxicology Research (CSIR) 80, M. G. Marg, Lucknow-226001, Uttar Pradesh, India.
2Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-244001, Uttar Pradesh, India.
Accepted 13 April, 2018
Abstract
Spoilage of food due to contamination by bacteria present in the environment is a major problem which affects human health and efforts to identify antibacterial components from natural sources has gained momentum. In this regard we isolated a new strain of BACILLUS LICHENIFORMIS IITRHR2 (accession number FJ447354) which produced bacteriocin like inhibitory substance (BLIS). Partially purified BLIS showed a major peak on HPLC with antimicrobial activity. This proteinaceous substance (~1.2 kDa) was thermo-stable and pH resistant but lost activity when subjected to proteinase treatment. BLIS inhibited growth of 16 bacteria including environmental and clinical contaminants of food products and potentiated the activity of nisin. In conclusion, the proteinaceous substance isolated from B. LICHENIFORMIS IITRHR2 showed potential to combat contamination of food products by bacteria present in the environment.
Key words: Antimicrobial activity, Bacillus licheniformis IITRHR2, BLIS, nisin.
Sapna Sharma, Ram L. Singh and Poonam Kakkar*
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-004, June, 2018. © International Scholars Journals
Full Length Research Paper
Characterization and antibiogram of Salmonella spp. from poultry specimens
R. Selvaraj1, R. Das1, S. Ganguly2, M. Ganguli3, S. Dhanalakshmi3 and S. K. Mukhopadhayay3*
1Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), Kolkata 700 037, India.
2AICRP on Post Harvest Technology in Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), Kolkata 700 094, India.
3Department of Veterinary Pathology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), Kolkata 700 037, India.
Accepted 13 March, 2018
Abstract
The present study was done for characterization and antibiotic sensitivity pattern of the Salmonella spp. found as contaminants in chicken carcass. The overall incidence of Salmonella contamination of poultry carcass was found to be 4.90% with the higher percentage of Salmonella being isolated from chicken meat (8.00%) followed by liver and spleen (6.25%). The isolates were identified as Salmonella Typhimurium, Salmonella Paratyphi B and Salmonella (Rough). Eight Salmonella isolates obtained from poultry were confirmed as Salmonella spp. according to their biochemical profile and their sensitivity to different antimicrobial agents. Amikacin, kanamycin and ciprofloxacin were found to be the most effective antibiotics against Salmonella spp.
Key words: Characterization, Salmonella spp., chicken carcass, antibiotic sensitivity.
R. Das, S. Ganguly, R. Selvaraj, M. Ganguli, S. Dhanalakshmi and S. K. Mukhopadhayay*
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-005, June, 2018. © International Scholars Journals
Full Length Research Paper
Symptomatic urogenital candidiasis and therapeutic management on women taking oral contraceptive pills
Jombo G. T. A.1*, Akpera M. T.2, Adebisi S. I.3 and Bolarin D. M.4
1Department of Medical Microbiology and Parasitology, College of Health Sciences, Benue State University, P. M. B. 102119, Makurdi, Nigeria.
2Department of Haematology, College of Health Sciences, Benue State University, P. M. B. 102119, Makurdi, Nigeria.
3Department of Chemical Pathology, College of Health Sciences, Benue State University, P. M. B. 102119, Makurdi, Nigeria.
4Department of Chemical Pathology, College of Medicine, Lagos State University, Lagos, Nigeria.
Accepted 13 March, 2018
Abstract
The use of oral contraceptive pills are increasingly becoming popular among women in both urban and rural Nigerian settings, and its perceived association with gynaecologic infections not withstanding. This study was therefore carried out to ascertain the rate of urogenital candidiasis among women on oral contraceptive pills (OCP) in Gboko town. All the willing women on OCP attending family planning clinic and Comprehensive health centre in Gboko were consecutively recruited in the month of September, 2009. Questionnaires were used to obtain relevant data such as age, marital status, occupation and urogenital symptoms. Urine, high vaginal swab and endocervical swab specimens were subsequently collected, transported and processed for isolation of microorganisms using standard laboratory procedures. The rate of urogenital candidiasis among the 153 women on OCP was significantly higher 36.5% compared to the control 20.3% (P < 0.05) in as much as their general knowledge about the disease was poor; similarly, genitourinary symptoms were recorded in 22.2% of the women on OCP as compared to the 5.2% in the control group (P < 0.001) and was significantly higher among the singles, separated, widowed and divorced (64.4 to 64.7%) compared to the married 27% (P < 0.05). 79.4% of the symptomatic infections were caused by Candida species. Women should be properly counselled and health educated on the need for prompt and adequate treatment of vulvovaginal candidiasis while facilities for appropriate treatment and proper laboratory diagnosis provided.
Key words: Candidiasis, contraceptive pills, oral, urogenital, women.
Akpera M. T, Jombo G. T. A*, Adebisi S. I and Bolarin D. M
Page: 1 - 5
Short Communication
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-003, June, 2018. © International Scholars Journals
Short communication
Vancomycin resistant enterococcus causing surgical site infections in a tertiary care hospital
J. B. Suchitra1* and N. Lakshmidevi2
1Freedom Foundation, 103, 1st stage, 1st block, HBR layout, Bangalore, India.
2Department of Studies- Microbiology, Manasagangothri, Mysore University, India.
Accepted 20 February, 2018
Abstract
Presence of Vancomycin Resistant Enterococcus (VRE) is increasingly being reported. In the present study we reported the prevalence of Vancomycin Resistant Enterococcus causing Surgical Site Infections (SSI) in a tertiary care hospital. Pus from surgical site wound was cultured and Enterococcus species was identified as standard microbiological methods. The antimicrobial susceptibility for Vancomycin was determined by Minimum Inhibitory Concentration (MIC) by broth dilution method. Patients (n = 2566) who had undergone surgery were included in the study. Enterococcus species had grown in cultures in 112 (4.3%) patients. Vancomycin resistance was observed in 9 isolates (8.03%). The rampant use of higher antibiotics in hospital has already paved way for multi drug resistance. The presence of Vancomycin Resistance must caution both physicians as well as hospital administrators on the urgent need for stringent hospital infection control programmes.
Key words: Vancomycin resistant enterococcus, VRE, surgical site infection.
J. B. Suchitra*, N. Lakshmidevi
Page: 1 - 3
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-008, June, 2018. © International Scholars Journals
Full Length Research Paper
Multiple-mutations in the katG encoding catalase proxidase in isoniazid resistant Mycobacterium tuberculosis isolates
M. Karim Rahimi3 S. Zaker bostanabad1, 3, 4*, P. Adimi3, M. Shekarabei4, 6, M. Habibollah4, F. Shirmohammadi4, Kh. Bigdeli4, A. Faraji4, B. Delalat4, Z. Tayebi3, M. Masoumi3, E.
Jabbarzadeh2, Sh. Pourazar2 and L. P. Titov5
1Islamic Azad University, Parand Branch, Biology and Microbiology Department, Iran.
2Pasteur Institute of Iran, Mycobacteriology and Pulmonary Research Department, Iran.
3Islamic Azad University, Tehran Medical Branch, Microbiology Department, Iran.
4Masoud Laboratory, Microbiology Department, Islamic Azad University, Iran.
5Belarusian Research Institute of Epidemiology and Microbiology, Clinical Microbiology, Belarus.
6Iran Medical University, Immunology Department, Iran.
Accepted 22 March, 2018
Abstract
The aim of this study was to investigate the significance of multiple-mutations in the katG gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic regions of Iran. Drug susceptibility testing was determined using the CDC standard conventional proportional method. DNA extraction, katG gene amplification and DNA sequencing analysis were performed. Thirty four (80%) isolates were found to have multiple-mutations (composed of 2 - 5 mutations) in the katG gene. Increased number of predominant mutations and nucleotide changes were demonstrated in codons 315 (AGC ACC), 316 (GGC AGC), 309 (GGT GTT) with a higher frequency among patients bearing secondary tuberculosis infection with elevated levels of resistance to isoniazid (MIC µg/ml 5 - 10). Furthermore it was demonstrated that the combination of mutations with their predominant nucleotide changes were also observed in codons 315, 316 and 309 indicating higher frequencies of mutations among patients with secondary infection respectively. In this study 62% (n = 21) of multi-mutated isolates found to have combination of mutations with predominant nucleotide changes in codons 315 (AGC ACC), 316 (GGC GTT), 309 (GGT GGT) and also demonstrated to be more frequent in isolates of patients with secondary infections, bearing higher level of resistance to isoniazid ( 5 – 10 µg/ml).
Key words: Predominant mutation, Mycobacterium tuberculosis, high level resistant to izoniazid, Iran.
Z. Tayebi, M. Karim Rahimi S. Zaker bostanabad*, M. Habibollah, Sh. Pourazar and L. P. Titov, A. Faraji, Kh. Bigdeli, E. Jabbarzadeh, F. Shirmohammadi, B. Delalat, P. Adimi, M. Masoumi, M. Shekarabei
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