ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Effects of methyl jasmonate in regulating cadmium induced oxidative stress in soybean plant (Glycine max L.)
Batool Keramat1, 2*, Khosrow Manouchehri Kalantari1,2 and Mohammad Javad Arvin3
1Biology Department, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran.
2International Center for Science, High Technology and Environmental Sciences, Kerman, Iran.
3Horticalture Department, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
Accepted 19 February, 2013
Abstract
The jasmonates are a group of plant–growth regulators that cause a wide variety of plant responses. Cadmium (Cd) is a toxic heavy metal, which causes toxicity in living organisms, and is considered as an environmental contaminant. In present investigation, the interactive effects of methyl jasmonate (0, 0.01 or 0.1 mM) and cadmium chloride (0 or 500 µM) on malondialdehyde (MDA), hydrogen peroxide (H2O 2) and antioxidant enzymes in soybean (Glycine max L.) leaves were studied. Treatment of methyl jasmonate (MeJA) together with cadmium reduced the amount of H2O2 and MDA when compared with cadmium treated plants. The activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT), increased in treated plants with MeJA, and Cd, but guaiacol peroxidase (GPX) activity did not show any noticeable difference. In addition, MeJA treatment increased the amount of dehydro ascorbic acid (DHA) in plants under Cd stress whereas, there was no significant difference in the content of ascorbic acid (ASA). Based on our results, it seems that the application of MeJA together with Cd caused alleviation of Cd damages by the reduction of MDA and H2O2 content and increase in activities of antioxidant enzymes in soybean plants.
Key words: Ascorbate peroxidase, catalase, lipid peroxidation, superoxide dismutase.
Batool Keramat*, Khosrow Manouchehri Kalantari and Mohammad Javad Arvin
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Antihyperglycemic and antihyperlipidemic effects of Clitoria ternatea Linn. in alloxan-induced diabetic rats
P. Daisy1, Kanakappan Santosh2 and M. Rajathi3*
1Department of Biotechnology, Holy Cross College, Tiruchirappalli-620002. Tamilnadu, India.
2Physical Oceanography Research Laboratory, Tropical Marine Science Institute
12 A Kent Ridge Road, Singapore.
3Department of Biotechnology, MVJ College of Engineering, Near ITPL, Channasandra, Bangalore-560067, Karnataka, India.
Accepted 15 February, 2013
Abstract
This study aims to investigate the therapeutic effects of Clitoria ternatea Linn. leaves and flowers extract on alloxan-induced diabetic rats. The effect of aqueous extract of C. ternatea leaves and flowers on serum glucose, glycosylated hemoglobin, insulin, total cholesterol, triglycerides, HDL-cholesterol, protein, urea, creatinine were examined in control and extract treated diabetic rats. Glycogen was examined both in the liver and skeletal muscles of control and extract treated diabetic rats, whereas, the activity of glycolytic enzyme glucokinase and gluconeogenic enzyme glucose-6- phosphatase was examined in the liver. Oral administration of aqueous extract of C. ternatea leaves (400 mg/kg body weight) and flowers (400 mg/kg body weight) for 84 days significantly reduced serum glucose, glycosylated hemoglobin, total cholesterol, triglycerides, urea, creatinine and the activity of gluconeogenic enzyme glucose-6-phosphatase, but increased serum insulin, HDL-cholesterol, protein, liver and skeletal muscle glycogen content and the activity of glycolytic enzyme glucokinase. For all the above biochemical parameters investigated, C. ternatea leaves treated rat showed a little better activity than C. ternatea flowers treated diabetic rats. The present investigation suggests that C. ternatea leaves and flowers extract exhibit antihyperglycaemic and antihyperlipidaemic effects and consequently may alleviate liver and renal damage associated with alloxan-induced diabetes mellitus in rats.
Key words: Alloxan, diabetes mellitus, Clitoria ternatea, enzymes, hemoglobin.
P. Daisy, Kanakappan Santosh and M. Rajathi*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
In vitro antimicrobial activity of the extracts from the leaves of Chrysocoma ciliata L.
A. O. T. Ashafa* and A. J Afolayan*
Department of Botany, University of Fort Hare, Alice, South Africa.
Accepted 6 May, 2013
Abstract
Chrysocoma ciliata L. is a medicinal plant used in the management of pains, stomach and menstrual disorders in the Eastern Cape province of South Africa. Studies were conducted to determine the antimicrobial activity of the extracts from this herb. All the extracts except water extract, inhibited Staphylococcus aereus, Staphylococcus epidermidus, Bacillus cereus and Streptococcus faecalis at minimum inhibitory concentration (MIC) less than 0.1 mg/ml. The extracts also inhibited the gram-negative bacteria tested including the popularly known antibiotic resistant Pseudomonas aeruginosa with MIC ranging from 1.56 to 12.5 mg/ml. All the extracts at the concentration of 0.5 mg/ml, suppressed the growth of Aspergillus niger, Aspergillus flavus and Penicilium notatum with percentage inhibition ranging from 50.83 to 88.33%. The ethanol and methanol extracts were able to inhibit the growth of Candida albicans at 5.0 mg/ml. Results from this study have shown that extracts from C. ciliata displayed strong antimicrobial activity, which is a manifestation of the plant’s broad spectrum potential for the treatment of microbial induced ailments including complications and disorders associated with the female reproductive and genital organs. This herb could be a potential agent for antibiotic bioprospecting.
Key words: Chrysocoma ciliata, medicinal plant, menstrual disorder, antimicrobial activity, bioprospecting.
A. J Afolayan*, A. O. T. Ashafa*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Experimental study on antimicrobial activity of cotton fabric treated with aloe gel extract from Aloe vera plant for controlling the Staphylococcus aureus (bacterium)
D. Jothi
Textile Engineering Department, Bahir Dar University, Bahir Dar, Ethiopia. E-mail:[email protected].
Accepted 29 April, 2013
Abstract
Biotechnology is a frontier area in science and technology having significant commercial applications in healthcare, agriculture, process industry and service sectors the world over. Ethiopia is in an advantageous position to harness the potential of biotechnology due to its unique strengths such as availability of technical expertise, skilled manpower, rich bio-resources and progressive government policies. The country is also rapidly emerging as a major market for biotech products. Today, new processes and products are closely linked with biotechnology in many research and industrial fields. There are the most diverse possibilities for its profitable use in the textile industry too. In this study, aloe gel has applied to 100% cotton fabric to develop antimicrobial fabric. In order to optimize the process parameter, cotton fabric was treated with Aloe vera extract (Aloe barbadensis Mill) at various concentrations that is 1, 2, 3 and 5 gpl at 60°C for 30 min by pad-dry –cure method. Methanol was used as a solvent for aloe gel extraction from Aloe vera plant. The finished fabric samples have been tested for activity as per the ATCC (Agar diffusion) method and quantitative analysis test method. The aloe gel treated fabrics exhibited antimicrobial activity against the Staphylococcus aureus (ATCC 6538). The treated cotton fabrics have shown excellent antimicrobial activity at 5 gpl concentration. The wash durability of the treated sample was found good even after 50 wash.
Key words: Biotechnology, agar plate, micro organism, Aloe vera.
D. Jothi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Characterization of lactic acid bacteria producing bacteriocins against chicken Salmonella enterica and Escherichia coli
N. L. Tatsadjieu1*, Y. N Njintang3, T. Kemgang Sonfack2, B. Daoudou2 and C. M. F. Mbofung2
1Laboratory of Microbiology, IUT, University of Ngaoundere, Cameroon.
2Laboratory of Microbiology, ENSAI, University of Ngaoundere, Cameroon.
3Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Cameroon.
Accepted 06 April, 2013
Abstract
30 strains of lactic acid bacteria (LAB) isolated from 5 samples, raw and fermented cow milk, cow meat, soil and fermented-dried cassava, were screened for their antimicrobial activity against poultry pathogens Salmonella enterica CIP8132 and Escherichia coli CIP548. All the lactobacillus strains were active against the target pathogens with the strains LF2, SM3, SM4, V3 and V5 exhibiting activity higher than 25 mm. 4 of the 5 isolated cultures were confirmed as bacteriocin producing cultures, in addition LF2 strain was cocci, can grow on sucrose, was thermal resistant and was partially inhibited by proteinase K and lysozyme suggesting a nisin-like molecule. This strain with promising future needs to be further characterised.
Key words: Lactic acid bacteria, antimicrobial activity, bacteriocins, Salmonella enterica, Escherichia coli.
B. Daoudou and C. M. F. Mbofung, Y. N Njintang, N. L. Tatsadjieu*, T. Kemgang Sonfack
Page: 1 - 10
Research Article
S. C. Enemuor*, A. S. Amedu
Page: 1 - 10