ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Evaluation of specimens in which the urine sediment analysis was conducted by full-automatic systems and a manual method together with urine culture results
Okhan Kadir AKIN1, Muhittin A. SERDAR2*, Zeynep CIZMECI3 and Ozlem GENC3
1Kecioren Research and Training Hospital, Clinical Chemistry and Laboratory Science, Ankara, 06380, Turkey.
2Gulhane School of Medicine, Department of Clinical Chemistry, Ankara, 06018, Turkey.
3Kecioren Research and Training Hospital, Microbiology and Clinical Microbiology, Ankara, 06380, Turkey.
Accepted 15 January, 2019
Abstract
The aim of the present study is to assess leukocyte and bacterial counts, and urine strip leukocyte esterase and as well as nitrite results from IQ200 and UriSed full-automatic urine sediment analyzers, with urine culture results. Six hundred urine cultures were performed in accordance with the routine laboratory procedures of the hospital. A growth <104 CFU/mL in the culture was considered negative. After the inoculation of urine samples into the culture media, urine samples were analyzed by IQ200 and UriSed full-automatic urine analyzers, respectively. As 104 CFU/mL are considered the diagnostic criterion for UTIs, 39 (6.5%, n=600) urine specimens yielded positive cultures. The positive likelihood ratios (PLR) of the IQ200 were suitable for bacteria and nitrite measurements (PLR>10); however, negative likelihood ratios (NLR) were inadequate for both devices and the manual method (NLR >0.3 for all). However, when the utilization of two tests in combination was analyzed, the diagnostic odds ratio was 48 for the IQ200 (95% confidence interval CI, 12.1-190) and 168 for the UriSed (95% CI, 20.6-1369) in cases in which leukocyte, bacteria, and nitrite measurements were high together. The results of the IQ200, UriSed, and urine strip test do not accurately reflect the urine culture results. Nevertheless, it was concluded that negative nitrite and leukocyte results of both automated and manual tests could be utilized in ruling out a urinary tract infection (UTI) diagnosis.
Key words: IQ200, UriSed, KOVA® system, full-automatic urine sediment analysis, bacteriologic urine culture, urine microscopy.
Muhittin A. SERDAR*, Okhan Kadir AKIN, Zeynep CIZMECI and Ozlem GENC
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
What changed in necrotizing fasciitis in twenty-five years?
Baris Yildiz1*, Derya Karakoc2, Erhan Hamaloglu2, Arif Ozdemir2 and Ahmet Ozenc2
1Ankara Numune Teaching and Research Hospital, 6th General Surgery, Ankara, Turkey.
2Department of General Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Accepted 11 January, 2019
Abstract
Necrotizing fasciitis (NF) is a deadly soft tissue infection characterized by necrosis of subcutaneous tissues. In this study, our aim was to identify variables affecting patient outcome and mortality in necrotizing fasciitis and their temporal changes. We reviewed records of 45 patients treated at our institution between 1979 and 2004. Data about gender, age, etiology, site of involvement, bacteriology, type of surgery, supportive treatment, accompanying diseases, mortality were collected. Factors contributing to mortality were sepsis, renal failure, liver failure, multi organ failure, disseminated intravascular coagulopathy and long term intubation. Mortalities accumulated in first 23 patients. There was not difference in microbiology, demographics, etiology, site of involvement, debridement technics between first patients and recent patients of the institution. Mortality in necrotizing fasciitis is mostly because of sepsis and associated disorders. Adequate control of the microbiological agent and preventing further contamination of the wound is cardinal part of treatment in NF.
Key words: Necrotizing fasciitis, bacteriology, mortality, sepsis, Fournier’s gangrene.
Derya Karakoc, Baris Yildiz*, Erhan Hamaloglu, Arif Ozdemir and Ahmet Ozenc
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Nigerian phages: The first bacteriophages from Tropical Africa
Adeniran S. Koko1,3, Hans W. Ackermann2*, Monisola A. Taiwo1 and Sunday A. Omilabu1
1Department of Botany and Microbiology, University of Lagos, Lagos, Nigeria.
2Félix d'Hérelle Reference Center for Bacterial Viruses, Department of Microbiology, Faculty of Medicine, Laval University, Québec, QC, G1K 7P4, Canada.
3Quest Diagnostics West Hills, CA 91304, CA, USA.
Accepted 03 March, 2019
Abstract
Fifteen morphological bacteriophage types were observed in enrichment cultures of sewage, surface water and human feces collected in Lagos, Nigeria. All phages were tailed and belonged to the Myoviridae, Siphoviridae or Podoviridae families. Most phages could be assigned to well-known morphotypes. Ten of them corresponded to phages of enterobacteria or pseudomonads. Four morphotypes (φKZ, T4 and χ, Jersey) prevailed in most samples. Most others occurred in traces only.
Key words: Bacteriophages, ecology, identification, morphotypes.
Adeniran S. Koko, Hans W. Ackermann*, Monisola A. Taiwo and Sunday A. Omilabu
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
Ephedra alata as biologically-based strategy inhibit aflatoxigenic seedborne mold
Al-Qarawi, A. A.1, Abd_Allah, E. F.1* and Hashem Abeer2
1Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Saudi Arabia.
2Department of Botany and Microbiology, Faculty of Science, King Saud University, Saudi Arabia.
Accepted 11 January, 2019
Abstract
Investigation has been carried out to evaluate the effect of range plant Ephedra alata as biologically-based strategy inhibit growth characters and aflatoxin production (in vitro and in vivo) of Aspergillus flavus. The aqueous extract of E. alata had significant inhibitory potential against growth as well as aflatoxin production by aflatoxigenic seedborne mold (Aspergillus flavus). Moreover, it has been found that, the addition of 1 and 2% (w/w) of plant powder material of E. alata to corn grains and soybean seeds respectively decreased the aflatoxin contamination and improve their nutritional value (total nitrogen content, fiber content, total lipids content and ash content) under storage conditions. The results observed here suggest employment of E. alata as an alternative non-chemical mean to control aflatoxin contamination of fodder in Saudi Arabia.
Key words: Biocontrol, Aflatoxins, Aspergillus flavus, Ephedra alata, nutritional value, Saudi Arabia.
Al-Qarawi , Abd_Allah , A. A, E. F* and Hashem Abeer
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
A simple and efficient method that uses low concentration fetal bovine serum to culture and purify Schwann cells
Han Feng, Qu Wei*, Jiang Huajun, Fu Chongyang, Lu Ming, Zhang Weiguo and Lv Decheng
Department of Orthopaedic Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, China.
Accepted 18 February, 2019
Abstract
To develop a simple and efficient method for the isolation of highly purified Schwann cells (SCs) from the sciatic nerves of newborn rats, cells were initially cultured in 10% FBS for 6 days, and then cultured in 2% FBS for 6 days. And we used the immunocytochemical characterization, flow cytometry, RT-PCR, to observe the condition of SCs. After the 12-day treatment protocol, we obtained a high yield of SCs with an average purity of 96.9%. Furthermore, the condition of SCs had no change. In our purification protocol, we had shown that our procedures did not alter the cell cycle and neurokines’ secretion. Our simple and efficient method yielded pure and healthy SCs and did not require treatment with anti-mitotic agents or any special equipment.
Key words: Schwann cells, cell culture, low fetal bovine serum concentration, tissue engineering, fibroblast.
Han Feng, Qu Wei*, Fu Chongyang, Zhang Weiguo and Lv Decheng, Lu Ming, Jiang Huajun
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-007, March, 2019. © International Scholars Journals
Full Length Research Paper
Screening of antibiotic sensitivity, antibacterial and enzymatic activities of microbes isolated from ex-tin mining lake
Ong K. S.1, Chin H. S.2 and Teo K. C.1*
1Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Malaysia.
2Science and Technology Innovation Center, Microbiology Laboratory, Ansell Shah Alam Sdn. Bhd. Malaysia.
Accepted 11 February, 2019
Abstract
Water sample from ex-tin mining lake was collected and cultured on nutrient agar (NA). Morphology characteristics revealed that isolates WL1 and WL4 were Gram-negative cocci and WL3 was Gram-positive cocci. Meanwhile, WL2 and WL5 were Gram-positive bacilli and spore formers. These isolates were screened for their antibiotic sensitivity against 17 types of antibiotics using Kirby-Bauer method. Antibacterial activity against 11 types of indicator bacteria were also tested using agar well diffusion method. The antibiotic susceptibility test demonstrated that all five isolates had high degree of inhibition to five types antibiotics namely amikacin, kanamicin, gentamicin, norfloxacin and tetracycline. Gram-negative cocci WL4 is a multiple resistant isolate that resistant to 10 types of antibiotics. The Gram-positive bacilli WL2 had antibacterial activity against Gram-negative ESCHERICHIA COLI. Enzymatic assay showed that all isolates were proteolytic, lipolytic and phosphatase enzyme producers. Amylolytic activity was absent in all isolates. Protease, leucine arylamidase, alkaline and acid phosphatases, phospholipase esterase and esterase lipase were found in all isolates. The preliminary survey demonstrated that the water environment in the vicinity of Campus Lake is a reservoir of microbial populations. The present work concerns a screening of a few selected isolates with some significance importance in the application of medical and industry fields. In conclusion, the multi resistance isolates were not suitable for industry application due to some facts that it might produce toxic compounds and cause infection. The antimicrobial substance and enzymes produced by the potential isolates could be further screened for clinical application and mass production in industry. However, they must be evaluated for their safety and toxicity to human, animals and the environment.
Key words: Nutrient agar (NA), Kirby-Bauer, antibacterial assay, antibiotic susceptibility test, enzymatic assay.
Chin H. S and Teo K. C*, Ong K. S
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