ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Comparison of cell growth and ethanol productivity on different pretreatment of rice straw hemicellulose hydrolysate by using Candida shehatae CICC 1766
Wan-Li Sun1,2 and Wei-Yi Tao1*
1Key Lab of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
2College of Chemistry and Bioengineering, Yichun University, Yichun, China.
Accepted 10 May, 2015
Abstract
In this work, the cell growth and ethanol productivity was tested for lignocellulosic biomass sugars of overliming-detoxified and NaOH-neutralized rice straw acid hydrolysate by using Candida shehatae CICC 1766. When the acetic acid reached 3 g/L in defined xylose medium, the final ethanol concentration was 33% of that in the medium without acetic acid addition. C. shehatae CICC 1766 could bear lower pH (ethanol yield 13.5 g/L at pH 4.0) in overliming-detoxified hydrolysate. At the optimal pH 5.0 the ethanol yield attended to 16.1 g/L. Ethanol yield in NaOH-neutralized hydrolysate was 13.7 g/L which is better than in overliming-detoxified hydrolysate (7.0 g/L) . It suggested that NaOH-neutralized hydrolysate could be directly fermented by using C shehatae CICC 1766.
Key words: Rice straw, Candida shehatae, hemicellulose hydrolysate, ethanol fermentation.
Wei-Yi Tao, Wan-Li Sun
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Evaluation of serum copper level during Giardia intestinalis infection
Eser Kilic1, Recep Saraymen1, Ozlem Miman2 and Suleyman Yazar3*
1Department of Biochemistry and Clinical Biochemistry, Medical Faculty, Erciyes University, Kayseri, Turkey.
2Department of Microbiology, Medical Faculty, Afyon Kocatepe University, Afyonkarahisar, Turkey.
3Department of Parasitology, Medical Faculty, Erciyes University, Kayseri, Turkey.
Accepted 15 April, 2015
Abstract
Alterations of serum copper concentrations are commonly found in patients suffering from gastrointestinal infections and with hepatic, renal, cardiovascular and malignant diseases. In this study, we aimed to investigate the changes of total content of the essential element of copper level in patients infected with Giardia intestinalis. Copper concentration was measured in 64 patients who were positive for intestinal parasite of G. intestinalis. Scores obtained for the positive samples, their age and sex, matched 60 G. intestinalis negative healthy controls. The mean concentration of copper in serum was significantly higher in G. intestinalis positive subjects than in their controls both in females (p < 0.05) and males (p < 0.05). No correlation could be demonstrated between age and mean value of copper in G. intestinalis positive in both females and males control (p > 0.05). Copper level was found to be clearly increased in the patients infected with G. intestinalis compared to controls. Although, our findings suggest altered element metabolism due to the pathophysiologic changes of G. intestinalis, a detailed biochemical and molecular studies on large sample size are needed to put forward a relationship between G. intestinalis (may be other parasitic diseases) and serum element level changes.
Key words: Giardia Intestinalis, copper, atomic absorption.
Eser Kilic, Recep Saraymen, Ozlem Miman and Suleyman Yazar*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Seroprevalence of toxoplasmosis in high school girls in Bushehr city, South-west of Iran, 2009
Moradali Fouladvand*, Afshin Barazesh, Behrouz Naeimi, Keivan Zandi and Saeed Tajbakhsh
Department of Microbiology and Parasitology, Faculty of Medicine, The Persian Gulf Tropical and Infectious Diseases Research Center, Bushehr University of Medical Sciences, P. O. Box 3631, Bushehr, Iran.
Accepted 6 May, 2016
Abstract
Toxoplasmosis is a parasitic disease prevalent all over the world and it’s causal agent is Toxoplasma gondii. Toxoplasmosis is very important in pregnant women. Seroepidemiological survey of toxoplasmosis in young girls before marriage for identifying non immune girls could be used to prevent congenital toxoplasmosis. A seroepidemiological study of toxoplasma IgG-antibody in 516 high school girls was conducted. Sample cases were chosen randomly from public high school girls. Blood samples were collected and the obtained sera were examined for anti toxoplasma IgG and IgM antibodies by using ELISA method. Prevalence of toxoplasmosis in high school girls in Bushehr city was 22.1%. There was significant correlation between seropositivity of toxoplasma antibodies and factors such as, contact with cat, raw milk consumption, raw vegetables consumption and food consumption habits, but there was no significant correlation between seropositivity and availability of drinking water, keeping pet and information about toxoplasmosis. About 78% of high school girls in Bushehr city were seronegative in toxoplasma IgG antibody. It is recommended to the health managers to design the educational measures and develop the serological tests for identifying the non immune girls before marriage to prevent congenital toxoplasmosis.
Key words: Elisa, toxoplasmosis, high school girls, seroprevalence.
Behrouz Naeimi, Keivan Zandi and Saeed Tajbakhsh, Moradali Fouladvand*, Afshin Barazesh
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Isolating fungal endophyte from Paris polyphylla Smith var. yunnanensis and identifying their antibacterial ability
Q. Xuan1, F. K. Bao1, H. M. Pan2 and A. H. Liu3*
1Department of Microbiology and Immunology, Kunming Medical University, P.O. Box 650500 Kunming, China.
2Health Institute of Kunming Medical University, P. O. Box 650500 Kunming, China.
3Department of Biochemistry, Kunming Medical University, P. O. Box 650500 Kunming, China.
Accepted 25 February, 2015
Abstract
Eighteen fungal endophytes strains parasitizing the famous Chinese medicinal plant Paris polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz were isolated from surface-sterilized plant tissues, such as rhizome, root, stem, leaf and flower. The effect of endophytes on the growth of human pathogenic microbes was evaluated in vitro, using disc diffusion assay. According to the characteristics of cultures and DNA sequences, the WRF7 and WRF7’ belonged to the species Penicillium chrysogenum. The extract of WRF7’ inhibited a broad range of human -pathogenic bacteria, but that of WRF7 almost had no similar effect. And the metabolites of WRF7’ could not stop the growth of test fungus Candida albicans. The study indicates that P. chrysogenum becomes fungal endophyte of Paris polyphylla Smith var. yunnanensis and its isolate WRF7’ makes metabolites that are inhibitory to all test bacteria in vitro. Therefore, the host plant suppressing pathogens owing to its endophytes and inhibitory mechanisms of endophytes to test bacteria and fungi are different.
Key words: Fungal endophyte, Paris polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz, isolating, identifying, antibacterial ability.
H. M. Pan and A. H. Liu*, F. K. Bao, Q. Xuan
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Beneficial effects of Bacillus subtilis on field-grown tomato in Burundi: Reduction of local Fusarium disease and growth promotion
Venant Nihorimbere1,3, Marc Ongena1*, Hélène Cawoy1, Yves Brostaux2, Pascal Kakana3, Emmanuel Jourdan1 and Philippe Thonart1
1Walloon Centre of Industrial Biology, University of Liège, Gembloux Agro-Bio Tech, Belgium.
2Statistics, informatics and applied mathematics, University of Liège, Gembloux Agro-Bio Tech, Belgium. B-5030 Gembloux, Belgium.
3Faculty of Agronomy, University of Burundi, P. O. Box 1550, Bujumbura, Burundi.
Accepted 15 April, 2016
Abstract
The biocontrol potential of Bacillus subtilis S499 was evaluated on tomato in open field sites in low altitude area of the plain of Imbo in Burundi. This strain was tested in order to reduce the impact of an important fungal disease giving rise to large losses in local plantings. The causing pathogen was isolated from diseased leaves at different locations in the fields and identified as Fusarium most probably related to the semitectum species according to the fermentation profile, morphology and gene homology. Results of assays performed in two successive years on the same site indicated that bacterial treatment on seeds significantly increased growth and fruit yield of tomato plants and also provided a high level of protection against the disease caused by this Fusarium pathogen. This is the first reported study on this disease and based on the data collected, B. subtilis S499 may represent an effective solution as biocontrol agent where other chemical options have failed.
Key words: Bacillus subtilis, biocontrol, Fusarium disease, tomato plants.
Pascal Kakana, Yves Brostaux, Emmanuel Jourdan and Philippe Thonart, Hélène Cawoy, Marc Ongena*, Venant Nihorimbere
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full length Research Paper
Differentiation of Salmonella enterica based on PCR detection of selected somatic and flagellar antigens
Mohd Elbagir Elhassan Nori and Kwai Lin Thong*
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Accepted 22 April, 2015
Abstract
Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serotype a panel of 122 recently isolated human- and foodborne- Salmonella strains. The mPCR that targets the o (genes wzxC2, rfbJ, prt, tyv, wzxE, wzxC1, prt) and Vi (viaB) antigens successfully subtyped the strains into serogroups C2 (n = 35, 28.7%), B (n = 33, 27.1%), D9 (n = 29, 22.9%), E (n = 21, 17.2%), C1 (n = 2, 64%) and A (n = 2, 1.64%). Eight of the Salmonella strains from serogroup D were positive for Vi antigen. Two multiplex PCRs were optimized for detection of H1 antigens (Ha, Hb, Hd, r, z10, z6, g and m) and H2 antigens (1.5, 1.2, 1.6 and enx). Overall, the multiplex PCRs of O, H and Vi antigens results correctly serotyped 94 of 122 strains (77%). The most frequent serovars encountered were Salmonella weltevrerden, Salmonella enteritidis, Salmonella typhimurium, Salmonella hadar and Salmonella typhi. Application of DNA based technique for serogrouping and serotyping of the selected Salmonella enterica was found to be robust, quick, specific and reliable for the specific antigenic targets and is useful in the study area which lack complete serotyping facilities.
Key words: Salmonella, somatic antigens (O), flagella antigens (H), serotyping, multiplex PCR.
Kwai Lin Thong*, Mohd Elbagir Elhassan Nori
Page: 1 - 10