ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Production dynamics of Bacillus subtilis strain AG-1 and EAG-2, producing moderately alkaline proteases
Afia Ghafoor* and Shahida Hasnain
Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus University of the Punjab. 54590. Lahore, Pakistan.
Accepted 9 April, 2013
Abstract
Bacillus strains isolated from different habitats were screened and identified for high protease activity. Two of the bacillus strains genetically identified as Bacillus subtilis AG-1 and Bacillus subtilis EAG-2 exhibited tremendous potential for protease production. The strains were optimized with respect to production media and its components. Culture conditions were critically investigated with reference to other key determinants, involving inoculum concentration, incubation time, initial pH of the medium and temperature. The optimum production pH and temperature was found to be 7.0 and 35oC for B. subtilis AG-1, while 7.2 and 37oC were the optimum conditions for B. subtilis EAG-2. The inoculation ratio and incubation time were determined for both of the strains as 1% (v/v) and 24-32 h respectively. The study reveals that, high protease yield can be achieved over a moderate fermentation period with relatively economical production costs. Hence these strains might be useful for commercial exploitation.
Key words: Bacillus subtilis, protease, production medium, pharmamedia, optimum growth.
Shahida Hasnain, Afia Ghafoor*
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Research Article
Shanmugam Velmurugan*, Santhanam Rajagopal
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Influence of growth medium on diagnostic characters of aspergillus and penicillium species
M. E. Zain, A. A. Razak, H. H. El-Sheikh, H. G. Soliman and A. M. Khalil
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Accepted 27 January, 2013
Abstract
Three fungal strains; namely, Aspergillus terreus, Penicillium janthinellum and Penicillium duclauxii were cultured on different growth media including yeast extract, malt extract, yeast-malt extract, Czapek's Dox, Sabourod's, Harrlod's, and potato dextrose. The growth and secondary metabolites of the three fungal strains were greatly affected by the growth medium. The colour of the culture and secondary metabolites were noticeably altered and changed according to the growth medium used.
Key words: Growth medium, Aspergillus, Penicillium, Secondary metabolites, Culture characteristics.
H. G. Soliman and A. M. Khalil, H. H. El-Sheikh, M. E. Zain, A. A. Razak
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Isolation and partial characterization of bacteriocin produced from Lactobacillus plantarum
V. Karthikeyan* and S. W. Santosh
Department of biotechnology, School of Bioengineering, SRM University, Kattankulathur-603203, Tamilnadu, India.
Accepted 19 February, 2013
Abstract
Bacteriocin producing Lactobacillus plantarum strain isolated from marine shrimp (Penaeus monodon) gut, showed broad range of antibacterial activity against some major food born pathogens. Maximum bacteriocin production was observed at 50°C, pH 4 and 0.9% sodium chloride solution. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 2.5 KDa based on the plasmid curing experiment, suggesting that the bacteriocin was a plasmid encoded protein molecule. The study revealed the possibility of using bacteriocin as a food preservative and the L. plantarum strain as probiotic.
Key words: Antagonistic activity, bacteriocin, chromatography, Lactobacillus plantarum.
V. Karthikeyan*, S. W. Santosh
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Research Article
Gurvinder Kaur*, V. Padmaja
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Effects of rockphosphate and arbuscular mycorrhizal fungi on growth and nutrition of Sesbania sesban and Gliricidia sepium
Fatimata Ndiaye1,2*, Anicet Manga1,3, Gnagna Diagne- Leye1, Samba Arona Ndiaye Samba2 and Tahir Abdoulaye Diop1,3
1Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Faculté des Sciences et
Techniques, Université Cheikh Anta Diop, B. P. 5005, Dakar, Sénégal.
2Laboratoire National de Recherches sur les Productions Végétales, Institut Sénégalais de Recherches Agricoles, Bel-
Air, B. P. 3120, Dakar Sénégal.
3Laboratoire Commun de Microbiologie IRD/ ISRA/UCAD, Centre de Recherches de Bel-Air, B. P. 1386, Dakar,
Sénégal.
Accepted 11 September, 2012
Abstract
A greenhouse experiment was conducted to evaluate the effects of different indigenous arbuscular mycorrhizal (AM) fungi on the mobilization of phosphorus from Senegalese natural rock phosphate (NRP) for growth of Gliricidia sepium and Sesbania sesban seedlings. Levels of tested NRP were compatible with high AM fungal proliferation but modified pattern of root colonization according to plant cultivar and fungal species. NRP applications and AM inoculation positively stimulated growth parameters and shoot mineral mass of G. sepium and S. sesban after four months cultivation. More than 200% of weight gains in S. sesban were recorded with all AM fungi combined with 600 or 800 mg NRP. With Gliricidia, only Glomus aggregatum in presence with these high NRP levels induced the same tendency. Glomus fasciculatum enhanced twice height growth of Sesbania in presence of 400, 600 and 800 mg NRP. The impact of dual application of AM fungi and NRP on nutritional content was more marked with Sesbania than in Gliricidia seedlings.
Key words: Rock Phosphate, AM fungi, Ghricidia sepium, Sesbania sesban.
Samba Arona Ndiaye Samba and Tahir Abdoulaye Diop, Gnagna Diagne- Leye, Anicet Manga, Fatimata Ndiaye*
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