ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-003, March, 2018. © International Scholars Journals
Short Communication
Inhibition of Astragalus membranaceus polysaccharides against liver cancer cell HepG2
Wu Yejin and Wang Yanqun*
School of Sport Science, Linyi Normal university,Shandong, 276600, PR China.
Accepted 02 January, 2018
Abstract
We have studied the inhibition of polysaccharides of Astragalus membranaceus against liver cancer cell HepG2. The polysaccharides at higher doses (25 mg/ml) have stronger antitumour effects, decreasing more than 40.5% (24 h) and 67.3% (48 h) of liver cancer HepG2 cell viability. A high percentage of apoptotic HepG2 cells was found at 25 mg/ml of A. membranaceus polysaccharides. 23.9 and 38.2% of cells experienced apoptosis when HepG2 cells were treated for 24 and 48 h with 25 mg/ml of A. membranaceus polysaccharides. Consequently, the results of the in vitro assays suggest that the A. membranaceus polysaccharides possesses strong antitumour activities, which is benefical to treatment of liver cancer.
Key words: Astragalus membranaceus polysaccharides, Antitumour, HepG2, MTT.
Wu Yejin, Wang Yanqun*
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Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-007, March, 2018. © International Scholars Journals
Full Length Research Paper
Antimicrobial effect of slightly acidic electrolyzed water for inactivation of Salmonella spp. and Escherichia coli on fresh strawberries (Fragaria L.)
Abdulsudi Issa-Zacharia1,2, Yoshinori Kamitani3, Happiness Muhimbula2 and Koichi Iwasaki3
1Laboratory of Food Biosystems and Environment, 1-21-24 Korimoto, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
2Department of Food Science and Technology, Sokoine University of Agriculture, P. O. Box 3006, Morogoro, Tanzania.
3Department of Environmental Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Accepted 24 January, 2018
Abstract
Antimicrobial effect of slightly acidic electrolyzed water (SAEW: pH 5.6 ± 0.1, 20.5 ± 1.3 mg/L available chlorine concentration; ACC) against indigenous aerobic mesophiles and inoculated Escherichia coli and Salmonella spp. on fresh strawberry was assessed. The antimicrobial effect of SAEW was compared with that of strong acidic electrolyzed water (StAEW) and sodium hypochlorite (NaOCl) solution. SAEW effectively reduced total aerobic mesophilic bacteria from strawberries by 1.68 log10CFU/g and was not significantly different from that of NaOCl solution (p > 0.05). Antimicrobial effect of SAEW against Salmonella spp. and E. coli was indicated by a more than 2 log10CFU/g reduction of their population and the effect was not significantly different from that of NaOCl solution and StAEW at similar treatment conditions (p > 0.05). From these findings, SAEW with a near- neutral pH and low available chlorine concentration exhibits an equivalent bactericidal effectiveness to NaOCl solution and thus SAEW is a potential sanitizer that would be used as an alternative for StAEW and NaOCl solution in the fresh fruit and vegetables industry.
Key words: Slightly acidic electrolyzed water, strawberry, total aerobic mesophilic bacteria, Escherichia coli, Salmonella spp
Yoshinori Kamitani, Happiness Muhimbula and Koichi Iwasaki, Abdulsudi Issa-Zacharia
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-005, March, 2018. © International Scholars Journals
Full Length Research Paper
Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis
Nazila Arbab Soleimani1*, Rooha Kasra Kermanshahi2, Bagher Yakhchali3 and Taher Nejad Sattari1
1Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of Microbiology, Alzahra University, Tehran, Iran.
3National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Accepted 17 January, 2018
Abstract
Staphylococcus aureus is one of the major pathogens which cause Bovine Mastitis (BM). Probiotic lactobacilli have the great potential to produce antimicrobial compounds that inhibit and control pathogenic bacteria. Antagonistic activity of probiotic lactobacilli ( L. acidophilus DSM 20079, L. plantarum ATCC 8014, L. casei ATCC 39392 and L. reuteri ATCC 23272) against S. aureus isolated from bovine mastitis (BM S. aureus) and standard S. aureus ATCC 25923 was the objective of this study. Antagonistic effect of probiotic lactobacilli was investigated by modified double layer method, well diffusion method, co -culturing assay and co-aggregation method. Among four lactobacilli, L. plantarum showed the greatest inhibitory activity. In modified double layer method the zone of inhibition of BM S. aureus and standard S. aureus ATCC 25923 by L. plantarum was 44 and 40 mm, respectively. Cell Free Supernatant (CFS) of probiotic lactobacilli in well diffusion method had inhibitory effect. Inhibition zone of BM S. aureus (13 mm) and standard S. aureus ATCC 25923 (9 mm) by L. plantarum was achieved. Co-culturing of L. plantarum with these two bacteria resulted in 87 and 77% inhibition growth of BM S. aureus and standard S. ureus ATCC 25923, respectively after 12 h. Co-aggregation between L. plantarum with two mentioned S. aureus was obtained 88.4 and 76%, respectively. According to these data, L. plantarum and its antimicrobial compounds can be one of the selective choices to control the BM S. aureus.
Key words: Antagonistic effect, probiotic lactobacilli, bovine mastitis, Staphylococcus aureus.
Nazila Arbab Soleimani*, Rooha Kasra Kermanshahi, Bagher Yakhchali and Taher Nejad Sattari
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-008, March, 2018. © International Scholars Journals
Full Length Research Paper
Effect of 60Co radiation on mesenchymal stem cells (MSCs) proliferation and differentiation
Xiaohui Ma*, Wei Guo, Xiaoping Liu, Tai Yin, Xin Jia, Jiang Xiong, Hongpeng Zhang and Lijun Wang
Departments of Vascular Surgery, Clinical Division of Surgery, Chinese PLA General Hospital and Postgraduate Medical School, BeiJing, 100853, PR China.
Accepted 20 January, 2018
Abstract
Mesenchymal stem cells (MSCs) are undifferentiated multipotent cells which reside in various human tissues and have the potential to differentiate into osteoblasts, chondrocytes, adipocytes, fibroblasts and other tissues of mesenchymal origin. In this study, we investigated the effect of 60Co radiation on the proliferation and differentiation of MSCs. MSCs were treated with increasing radiation doses to assess the effect on MSC. Results showed that 2 Gy of 60Co radiation did not significantly affect MSCs. When compared with the control group and 2Gy of 60Co radiation group, the MSCs viability after 4 Gy of 60Co radiation markedly decreased (p < 0.05). Two weeks of 8 and 12 Gy of 60Co radiation induced all cell death. After the 5-Aza treatment, the expression of myocardial- specific protein, C-TNT and -MHC was not detected in the MSCs which were pretreated by 60Co radiation. In conclusion, middle dose (4 Gy) of irradiation induces MSCs morphological changes, as well as alteration in both proliferation and differentiation potentials of MSCs. 5- Aza treatment did not induce both proliferation and differentiation of MSCs because high dose (8 and 12 Gy) of irradiation killed all cells.
Key words: Mesenchymal stem cells, radiation, proliferation, differentiation.
Hongpeng Zhang and Lijun Wang, Xiaohui Ma*, Xin Jia, Jiang Xiong, Wei Guo, Tai Yin, Xiaoping Liu
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-008, March, 2018. © International Scholars Journals
Full Length Research Paper
Infections and aerobic bacterial pathogens in diabetic foot
Ozer B.1*, Kalaci A.2, Semerci E.3, Duran N.1, Davul S.2 and Yanat A. N.2
1Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey.
2Mustafa Kemal University, School of Medicine, Department of Orthopedics and Traumatology, Hatay, Turkey.
3Toros Hospital, Mersin, Turkey.
Accepted 22 January, 2018
Abstract
The aim of the study was to investigate the causative pathogens, profile of antimicrobial susceptibility of them and the extent of tissue lesions in diabetic foot infections. This is a prospective study in which infected diabetic foot presenting with Wagner grade 1 to 5 ulcers were investigated. 78 consecutive diabetic patients who were seen in the orthopaedic clinic were cultured during ordinary visits. Bacteriological diagnosis and antimicrobial susceptibility profiles were carried out and analyzed using standard procedures. Diabetic polyneuropathy was found to be a common finding (74.4%). 15 (19.2%) cultures revealed polymicrobial involvement. The most frequent organisms isolated were Enterobacteriaceae (36.5%), Pseudomonas aeruginosa (18.9%), Enterococcus spp. (14.9%), and Staphylococcus aureus (10.8%). While imipenem, meropenem, amikacin, piperacillin/tazobactam were found out to be the most effective agents against Gram -negative organisms, vancomycin, teicoplanin, chloramphenicol were the most effective agents against Gram -positives. The aetiologies of most of the ulcers were neuropathic and 81.6% of them were deep. Our study also revealed that Gram -negative bacteria were the most common pathogens in infected diabetic feet. The diabetic foot ulcer is the most important cause of non-traumatic foot amputations so it is important to know the causative pathogens of these ulcers, profile of antimicrobial susceptibility of them for their treatment.
Key words: Diabetes mellitus, foot ulcers, infection, pathogen.
Duran N, Davul S and Yanat A. N, Ozer B*, Kalaci A., Semerci E
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-005, March, 2018. © International Scholars Journals
Full Length Research Paper
AdeABC efflux pump: Less important role in Acinetobacter baumannii against carbapenems
Jiansheng Huang1, Jinwei Huang1*, Fangyou Yu2, Xiaoguang Wang3 and Guoxiong Li1
1Department of Clinical Laboratory, Lishui Central Hospital, Zhejiang Province 323000, China.
2First Affiliated Hospital, Wenzhou Medical College, Zhejiang Province 325000, China.
3LiShui Center for Disease Control, Zhejiang Province 323000, China.
Accepted 28 October, 2017
Abstract
AdeABC efflux pump in a total of 50 Acinetobacter baumannii strains were investigated and the role in contributing to hydrolysis carbapenems were further analyzed. All strains were divided into 3 groups according to their susceptibilities to Imipenem, Amikacin, Minocycline and Levofloxacin: Group A (22 imipenem-resistant strains), B (13 isolates which were imipenem-sensitive but resistant to at least one of the other three antibiotics) and C (15 isolates, sensitive to all the antimicrobials). Five gene types were observed according to REP-PCR and 39 isolates were included in the main one. Only one isolate (A2) was positive for efflux pump phenotype. All strains were positive for blaOXA51-like and AdeB gene and negative for blaOXA24, blaOXA58, VIM, IPM, and SIM-1. blaOXA23 were detectable merely in the 22 imipenem-resistant strains. 24 isolates from the same REP type representing for the 3 groups were selected for quantitative analysis of adeB expression. Compared with the mean level of Group B, only A2 expressed apparently higher (2.2 fold). The induction effects of imipenem and meropenem were analyzed as well. Compared with their freely grew isogenic counterparts, up regulated expression was observed only in 2 isolates under the pressure of imipenem and none in all under meropenem. These data indicate that AdeABC efflux pump play a less important role in A. baumannii against carbapenems.
Key words: Acinetobacter baumannii, Carbapenem resistance, AdeABC efflux pump, adeB gene.
Xiaoguang Wang and Guoxiong Li, Fangyou Yu, Jiansheng Huang, Jinwei Huang*
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