ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 15 (1), pp. 001-005, January, 2021. © International Scholars Journals
Full Length Research Paper
Use of herbal extract for quantitative estimation of the antifouling activity in marine paint
A. M. Chandak*, Shastri P. N. and Gogte B. B.
Department of Food technology, Laxminarayan Institute of Technology, Nagpur University, Nagpur, India.
Accepted 22 November, 2020
Abstract
Biofouling and biofilm formations are of great concern to many modern industries, including marine, food, water, mining and medical field. The shipping industry has serious problems with biofouling (complex communities of marine organisms) on most surfaces submerged in seawater. This leads to problems such as increasing water resistance, fuel consumption and microbial corrosion of metal surfaces. The primary fouling agents on aquatic surfaces exposed to light happen to be algae and diatoms, whereas fungi and bacteria follow the trend, forming a biofilm on the surface. This further attracts the crustaceans and molluscs, which are responsible for macro-fouling. Various components such as tin, arsenic, mercury, lead and copper are used as the biocides. These components are polluting the environment affecting the marine life. The paper contents the use of herbal extract as the antifouling agent and approach for quantitative estimation of the antifouling activity of the Curcuma longa extract as compare to that of tin.
Key words: Antifouling, Curcuma longa, leaching, paint, Gleocapsa.
Shastri P. N. and Gogte B. B., A. M. Chandak*
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 15 (1), pp. 001-008, January, 2021. © International Scholars Journals
Full Length Research Paper
Assessment of Paramecium tetraurelia growth in a new synthetic culture medium
AZZOUZ Zoubir1,2*, BERREBBAH Houria2 and DJEBAR Mohamed Reda2
1Department of marine Sciences, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.
2Laboratory of Cellular Toxicology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000, B.P. 12. Algeria.
Accepted 22 August, 2020
Abstract
For using an organism for ecotoxicological studies, it is important to understand its model of growth under some physicochemical parameters that influence with a direct manner its development. The knowledge of the optimal parameters for the paramecia development leads to realize a model of growth with clear stages. For that, we tried to study the influence of temperature, pH, nutrient density in the culture medium and pollution on the growth of Paramecium tetraurelia. Obtained results showed that paramecia prefer definite conditions. However, the growth was better in temperatures included between 25 and 30°C, with preference to increased temperatures and a slightly acidic pH. The culture medium was prepared from the mixture of several vegetables which the rates were studied depending to the growth. The combined effects of azoxystrobin and cyproconazole on the growth kinetics were studied. Results showed that treatment affected the population growth, the generation time and the velocity of generation.
Key words: Paramecium tetraurelia, growth kinetics, physicochemical parameters, azoxystrobin, cyproconazole.
BERREBBAH Houria and DJEBAR Mohamed Reda, AZZOUZ Zoubir*
Page: 1 - 8
Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 14 (12), pp. 001-006, December, 2020. © International Scholars Journals
Full Length Research Paper
A study of the genome sequence of food-and-mouth disease virus WFL strain
Linzhu Ren1, Lin Wang3, Yunzhi Ma1* and Xinglong Wang2
1Heping Campus, Jilin University, 5333 Xi’an Road, Changchun, 130062, China.
2Department of Animal Food, The 11th Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun, China.
3Department of Pharmacology, School of Pharmacy, Second military medical university, No.800, Xiangyin Road, Shanghai, China.
Accepted 10 September, 2020
Abstract
The complete genome of the foot-and-mouth disease virus (FMDV) strain WFL was cloned and sequenced. The results showed that the complete genome was 8155 nucleotides (nt) in length (including the poly(C) tract, but excluding poly(A) tail) and was composed of a 1059-nt 5'-untranslated region (UTR), a 6969-nt open reading frame, and a 127-nt 3'- UTR. cre region of 5’UTR was 55nt with 45.5% of G/C, and had a stem-loop. The stem -loops region of 3’UTR can fold into two stem -loops, SL1 and SL2. A phylogenetic tree was constructed based on complete amino acids sequences of WFL strain and reference strains. The strains were divided into 4 clusters. O/ES/2001, HKN/2002, LZ and WFL strain can be divided into one group. It was obvious that WFL strain had a close relationship to LZ strain, which indicated that the WFL strain was of serotype O. There were 16 different deduced amino acid residues between the WFL strain and the LZ strain.
Key words: Food-and-mouth disease virus, sequence, complete genome, untranslated region (UTR).
Lin Wang, Yunzhi Ma* and Xinglong Wang, Linzhu Ren
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 14 (12), pp. 001-005, December, 2020. © International Scholars Journals
Full Length Research Paper
Classification of the BNC gene toward further understanding of the psychrophilic catalase
Wei Wang, Fang Wang, Xiaofeng Ji, Shanhong Liu, Cui Yuan, and Mi Sun*
Marine Products Resource and Enzyme Engineering Laboratory, Yellow Sea Fisheries Research Institute, 106 Nanjing Road, Qingdao 266071, Shandong Province, China.
Accepted 11 October, 2020
Abstract
The gene of psychrophilic catalase BNC from Antarctic Bacillus sp. N2a was cloned by degenerate PCR and inverse PCR. BNC gene revealed a 1,461 bp open reading frame for a protein with 486 amino acids. The phylogenetic tree based on the amino acid sequences of BNC and representative psychrophilic microbial catalases manifested that BNC belonged to the Group III of the monofunctional catalase. The active-site residues of the structure-determined catalase were highly conserved in BNC. Comparison of the amino acid composition of BNC with its mesophilic homologue from Bacillus subtilis TE124 showed that BNC had properties of a cold-active enzyme.
Key words: Antarctic Bacillus, monofunctional catalase, cold-adapted enzymes, gene cloning.
and Mi Sun*, Fang Wang, Wei Wang, Cui Yuan, Xiaofeng Ji, Shanhong Liu
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 14 (12), pp. 001-009, December, 2020. © International Scholars Journals
Full Length Research Paper
Evaluation of the impact of various tillage systems on rhizobiyal parameters
Kemal Dogan, Ismail Celik, Mustafa Gok and Ali Coskan*
Süleyman Demirel Üniversitesi, Ziraat Fakültesi Toprak Bölümü 32260 Çünür / Isparta, TÜRK YE.
Accepted 15 July, 2020
Abstract
The effects of different soil tillage methods on rhizobial nodulation, biomass yield (root, shoot and nodule) and nitrogen content of soybean as a second crop have been investigated. The experiment was conducted as a field trial in Cukurova Region, Turkey in 2008. Six different tillage methods have been studied in this research. These methods were conventional tillage with residue (CTR), conventional tillage with burnt residue (CTBR), reduced tillage with heavy disking (RTHD), reduced tillage with rotary tiller (RTR), no- tillage with heavy disking (NTHD) and no tillage with direct seeding (NTDS). At the stage of inflorescence, nodulation was investigated in the root, shoot, and nodule samplings by determining the weights of the samples and analyzing them for nitrogen contents. The results have been statically analyzed by using MSTAT-C package programme. According to the results of the study, under the conventional methods in which deep tillage operations are applied to soil, it has been found out that nodule formation and plant nitrogen contents have been negatively affected. The differences among the applications were found to be statistically significant. In general, the best nodulation has been obtained with NTDS and RTHD plots. Moreover, the effects of other tillage methods on some parameters related with rhizobiyal N2-fixation have caused to considerable decrease on the parameters mentined above.
Key words: N2-fixation, soil tillage methods, microbial activity.
Mustafa Gok and Ali Coskan*, Ismail Celik, Kemal Dogan
Page: 1 - 9
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (11), pp. 001-004, November, 2020. © International Scholars Journals
Full Length Research Paper
Phytochemical constituents of some Nigerian medicinal plants
H.O. Edeoga1*, D. E. Okwu2 and B.O Mbaebie1
Departments of 1Biological and 2Chemical Sciences, Michael Okpara University of Agriculture, Umudike P.M.B 7267,Umuahia, Abia State, Nigeria.
Accepted 21 February, 2020
Abstract
Alkaloids, tannins, saponins, steroid, terpenoid, flavonoids, phlobatannin and cardic glycoside distribution in ten medicinal plants belonging to different families were assessed and compared. The medicinal plants investigated were Cleome nutidosperma, Emilia coccinea, Euphorbia heterophylla, Physalis angulata, Richardia bransitensis, Scopania dulcis, Sida acuta, Spigelia anthelmia, Stachytarpheta cayennensis and Tridax procumbens. All the plants were found to contain alkaloids, tannins and flavonoids except for the absence of tannins in S. acuta and flavonoids in S. cayennsis respectively. The significance of the plants in traditional medicine and the importance of the distribution of these chemical constituents were discussed with respect to the role of these plants in ethnomedicine in Nigeria.
Keywords: Medicinal plants, ethnomedicine, phytochemical constituents.
H.O. Edeoga*, D. E. Okwu and B.O Mbaebie
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