ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (3), pp. 001-006, March, 2020. © International Scholars Journals
Full Length Research Paper
Development of a PCR-based method for detection of Nosema pernyi
Yi-Ren Jiang1,2, Zhen-Hua Deng2, Sheng-Lin Shi2, Rui-Sheng Yang2, Yan-Zhuo Li2, Yu-Xi Duan1* and Li Qin2*
1College of Plant Protection, Shenyang Agriculture University, Shenyang 110866, China.
2College of BioScience and Biotechnology, Shenyang Agriculture University, Shenyang 110866, China.
Accepted 10 October, 2019
Abstract
Nosema pernyi is the lethal pathogen of pebrine disease in Antheraea pernyi. We have developed a PCR-based method for detection of N. pernyi using specific primers. The primers were designed by the reported conserved regions of microsporidian SSU rRNA. When the genomic DNA of N. pernyi was used as the DNA template, the specific DNA sequences were amplified by PCR. It was observed that PCR diagnosis of N. pernyi using the three sets of primers provided increased specificity and sensitivity when compared with light microscopy.
Key words: Nosema pernyi, Antheraea pernyi, pebrine disease, spores, PCR, detection.
Yan-Zhuo Li, Sheng-Lin Shi, Zhen-Hua Deng, Rui-Sheng Yang, Yi-Ren Jiang, Yu-Xi Duan* and Li Qin*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (3), pp. 001-006, March, 2020. © International Scholars Journals
Full Length Research Paper
Research on the effect of Penicillium sp. on Luzhou-flavor liquor
Shangling Fang, Hong Liu , JiangBo Wang and MaoBin Chen*
Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
Accepted 19 October, 2019
Abstract
Penicillium sp. was isolated from Daqu as a beginner strain. In order to investigate the role of Penicillium sp. in Luzhou-flavor liquor, Penicillium sp. was operated in Koji seeds and then simulated solid-state fermentation for liquor testing under different conditions. They included adding of different amounts of koji seeds and Daqu. The results showed that, when the amount of Daqu added was 20%, Penicillium sp. koji added increased from 0.5 to 2%, compared to blank control group. Liquor yield was reduced by 2.23% from 29.77%, total ester decreased by 1.70% from 37.25%, total acid increased by 6.57% from 12.41% and four esters had different degrees of decrease trend. While the amount of koji seeds added exceeded 1.0%, ethyl lactate content was higher than ethyl caproate content. When the amount of Penicillium sp. koji added was 1.0%, yield of liquor, total esters, total acid and the four esters were all enhanced with the increase in amount of Daqu.
Key words: Penicillium sp., Daqu, Koji seeds, Luzhou-flavor liquor.
Hong Liu, Shangling Fang, JiangBo Wang and MaoBin Chen*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (3), pp. 001-010, March, 2020. © International Scholars Journals
Full Length Research Paper
Inhibition of Inula cappa (Ham. ex D. Don) DC. extracts on herpes simplex virus infection in vitro
Jiraporn Nikomtat1,3, Puttinan Meepowpan2 and Yingmanee Tragoolpua1*
1Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand.
2Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand.
3Department of Science, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000, Thailand.
Accepted 16 September, 2019
Abstract
The methanol and dichloromethane extracts of Inula cappa (Ham. ex D. Don) DC. were evaluated for inhibition of herpes simplex virus infection. Higher anti- herpes simplex virus (HSV) activity of I. cappa methanol extract was observed comparing with I. cappa dichloromethane extract since more than 50% of the virus was inhibited by I. cappa methanol extract and 50% effective doses of I. cappa methanol extract on HSV-1and HSV-2 were 720.1±32.7 and 529.2±5.2 µg/ml respectively. However, when the virus particles were treated with I. cappa dichloromethane and I. cappa methanol extracts, both HSV-1 and HSV-2 particles were directly inactivated to negligible amount within 1 - 2 h. More than 50% interference on blocking of HSV attachment and penetration was observed after treatment the virus with I. cappa methanol extract. Moreover, within 30 h, the effective inhibitory activity of I. cappa methanol extract on HSV replication was higher than that of the I. cappa dichloromethane extract. Both extracts had potent activities in reducing viral deoxyribonucleic acid (DNA) synthesis since HSV DNA after treatment with the extracts were 1.3-2 fold less than HSV DNA control after determination by real time polymerase chain reaction and approximately 45 kilodalton viral proteins were clearly diminished after treatment with I. cappa methanol extract. Therefore, methanol extract of I. cappa is a potent inhibitor of herpes simplex virus infection in vitro.
Key words: Anti-viral activity, Inula cappa, plant extract, herpes simplex virus.
Puttinan Meepowpan and Yingmanee Tragoolpua*, Jiraporn Nikomtat
Page: 1 - 10
Review
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (2), pp. 001-010, February, 2020. © International Scholars Journals
Review
Lactobacillus acidophilus cell structure and application
Parvaneh Jafarei1* and Maryam Tajabadi Ebrahimi2
1Islamic Azad University of Iran (IAU), Arak Branch, Arak, Iran.
2Islamic Azad University of Iran, Central Tehran Branch (IAU), Tehran, Iran.
Accepted 13 August, 2019
Abstract
Lactic acid bacteria (LAB) are one of the most applied bacteria in the production of fermented foods, from dairy to fruits and vegetables products. They make food durable, improve food safety, flavor, and texture as well as to enhance food physiological and hygienic value due to the presence of viable cells and valuable. Besides, products obtained by LAB fermentation processes are of special importance for functional foods such as probiotics. Lactobacillus acidophilus is the best well-known species of this Lactobacillus complex in LAB group and exist in the gastrointestinal tracts of humans and animals. In fermented food, the metabolic activity of this microorganism results in production of flavor, and aroma that cause organoleptic properties of fermented foods and inhibits foods spoilage. L. acidophilus effect on modulation of host immunity is proved by clinical evidence but molecular and cellular mechanisms of these effects are not completely clear. Despite the increasing application of this bacterium, little is known about its effects on the gut community, host physiology and immunity. The molecular mechanisms by which L. acidophilus exert these effects are not clearly understood. In this regard, recognition of cell structures and the genomic base of this bacterium could be useful. In this paper we try to gather all the information that exists about this bacterium.
Key words: Lactobacillus acidophilus, structure, fermented food, probiotic.
Maryam Tajabadi Ebrahimi, Parvaneh Jafarei*
Page: 1 - 10
Short Communication
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (2), pp. 001-003, February, 2020. © International Scholars Journals
Short Communication
Isolation and characterization of microsatellite loci for Rhyacionia leptotubula (Lepidoptera: Tortricidae)
Jia-Ying Zhu, Song Yang, Qin-Wen Li and Yong-He Li*
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, College of Forestry, Southwest Forestry University, Kunming 650224, China.
Accepted 20 September, 2019
Abstract
Microsatellite as one of the most popular molecular markers provides a useful tool for population genetic structure analysis in insects. But few microsatellite loci have been developed for Lepidoptera. Here we isolated and characterized 9 polymorphic microsatellite loci in Rhyacionia leptotubula (Lepidoptera: Tortricidae). The number of alleles of these loci ranged from 2 to 11. Observed and expected heterozygosities ranged from 0.0000 to 0.5294 and from 0.3905 to 0.8719, respectively. Seven loci show significant Hardy-Weinberg deviations. These loci could provide insight into population genetic structure and dispersal patterns of R. leptotubula.
Key words: Rhyacionia leptotubula, microsatellite, population genetics, Lepidoptera.
Song Yang, Qin-Wen Li and Yong-He Li*, Jia-Ying Zhu
Page: 1 - 3
https://doi.org/10.46882/ajmr/1013Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (2), pp. 001-006, February, 2020. © International Scholars Journals
Full Length Research Paper
Two methods for extraction of high-purity genomic DNA from mucoid Gram-negative bacteria
Xiao Xiao1, Jingling Zhang1,2, Quanyi Zhang1, Li Wang1,2, Yafang Tan1, Zhaobiao Guo1, Ruifu Yang1, Jingfu Qiu2 and Dongsheng Zhou1*
1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
2School of Public Health, Chongqing Medical University, Chongqing 400016, China.
Accepted 13 September, 2019
Abstract
Due to the production of abundant capsular polysaccharides and or exopolysaccharides, some bacteria form mucoid and opaque colonies on solid agar medium. Polysaccharides interfere with the isolation and characterization of DNA. Extraction of polysaccharide-free DNA from the mucoid bacteria is required for genetic and biochemical studies. In this study, mucoid Vibrio parahaemolyticus and Klebsiella pneumonia were used as model organisms for comparing the effectiveness of two DNA extraction methods. The two methods were proved reproducible and cost-effective for extracting high-purity genome DNA from mucoid bacteria for further applications.
Key words: Mucoid bacteria, Vibrio parahaemolyticus, Klebsiella pneumonia, DNA isolation, polysaccharides.
Jingfu Qiu and Dongsheng Zhou*, Xiao Xiao, Ruifu Yang, Yafang Tan, Jingling Zhang, Li Wang, Quanyi Zhang, Zhaobiao Guo
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