ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Efficacy of IgG, Fab, and F(ab')2 fragments of horse antivenom in the treatment of local symptoms after Cerastes cerastes (Egyptian snake) bite
Salwa S. Abdel Latif1*, Soheir Wanas1, George Abdel Malak1 and Madiha H. Helmy2
1Egyptian Organization for Biological Products and Vaccines (VACSERA), 51 Wezaret Elzeraa St., Agouza ,Giza
2Biochemistry Research Institute, Alexandria university, 165 ElHoria Road, El-Hadra region , Egypt.
Accepted 18 June 2003
Abstract
The ability of horse antivenoms, consisting of immunoglobulin G (IgG) and its fragments F(ab')2 and Fab were comparatively studied in mice to neutralize several effects of Cerastes cerastes venom. The three antivenoms were produced from the same batch of hyperimmune horse plasma. Neutralization was only partial when antivenins were administered intravenously at various time intervals after envenomation. No significant differences were observed among IgG, Fab, and F(ab')2 antivenoms concerning neutralization of hemorrhagic effects. Fab fragments were slightly more effective in neutralizing edema while IgG and F(ab')2 antivenoms were better in neutralizing myonecrosis in experiments involving independent injection of venom and antivenom.Thus these results disagree with the theory that "Fab " fragments are more effective than whole IgG and F(ab')2 in the neutralization of local symptoms accompanying C. Cerastes venom.
Key words: Cerastes cerastes, Egyptian snake, IgG, F(ab')2, Fab.
George Abdel Malak and Madiha H. Helmy, Soheir Wanas, Salwa S. Abdel Latif*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Analysis of AVR4 promoter by sequential response-element deletion
Olukosi, YA1 and Iwalokun, BA2*
1Genetics Division, Nigerian Institute of Medical Research (NIMR), 6, Edmond Crescent, Yaba – Lagos, PMB 2013, Lagos - Nigeria.
2Biochemistry Dept, Lagos State University, PMB 1087, Apapa – Lagos, Nigeria.
Accepted 24 June 2003
Abstract
Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2907-176, Avr4pBLCAT2809-176, Avr4pBLCAT2789-176, Avr4pBLCAT2429-176 and Avr4pBLCAT2302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities.
Key words: Avidin, AVR4 promoter, chloramphenicol acetyl transferase.
BA* , YA and Iwalokun, Olukosi
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1
S.T. Ogunbanwo*, A.I. Sanni, and A. A. Onilude
Department of Botany and Microbiology, University of Ibadan, Nigeria
Accepted 5 June 2003
Abstract
Bacteriocin produced by Lactobacillus brevis OG1 has large spectrum of inhibition against pathogenic, food spoilage microorganisms and various Lactic acid bacteria employed as test strains. The bacteriocin inhibited E coli NCTC 10418 and Enterococcus faecalis, but did not inhibit Candida albicans ATCC 10231 and Klebsiella sp. UCH 15. The antibacterial activity appeared to be pronounced between early logarithmic and early stationary phase. Supplementation and/or replacement of nutrients demonstrated that larger quantities of bacteriocin could be produced by addition of yeast extracts (3.0%), NaCl (1.0-2.0%), glucose (1.0 %) and Tween 80 (0.5%), while addition of tri-ammonium citrate, sodium acetate, magnesium sulphate, manganese sulphate and potassium phosphate had no effect on production. Maximal activity in composed medium was achieved at initial pH of 5.5, and incubation period of 48h at 30-370C.
Key words: Bacteriocin, growth media, Lactobacillus brevis OG1, indicator organisms, antagonistic activity.
and A. A. Onilude, S.T. Ogunbanwo*, A.I. Sanni
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Genetics similarity among four breeds of sheep in Egypt detected by random amplified polymorphic DNA markers
Bahy Ahmed Ali
Nucleic Acid Research Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research & Technology Applications, Alexandria, Egypt. E- mail: [email protected] or [email protected], Fax: 203 4593423
Accepted 5 June 2003
Abstract
A genetic analysis using RAPD markers was performed for studying variation in four breeds of sheep (Baladi, Barki, Rahmani and Saffolk). Nineteen random primers were used to amplify DNA fragments in these breeds. RAPD patterns with a level of polymorphism were detected between breeds. Results showed closer proximity of Barki to Rahmani and Baladi (95.7 and 91.3%), respectively
Keywords: Sheep, breeds, RAPD, genetic similarity.
Bahy Ahmed Ali
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Isolation, characterization, and phylogenetic analysis of copia-like retrotransposons in the Egyptian cotton Gossypium barbadense and its progenitors
Abdel Ghany A. Abdel Ghany1 and Essam A. Zaki2*
1Institute of Efficient Productivity, Zagazig University, Zagazig
2Genetic Engineering & Biotechnology Research Institute, GEBRI, Research Area, Borg El Arab, Post Code 21934, Alexandria, Egypt.
Accepted 16 May 2003
Abstract
We have used the polymerase chain reaction to analyze copia-like retrotransposons in the Egyptian cotton and its progenitors. All three cotton species studied contain reverse transcriptase fragments from copia-like retrotransposons. Sequence analysis of these reverse transcriptase fragments reveals that each is different from the others, with predicted amino acid diversities between 9 and 94%. The detection of stop codons and insertions/deletions in the derived amino acid sequences of the Gossypium RT clones, suggests that these clones represent defective retrotransposons. The presence of these sequences in G. barbadense progenitors, however, suggests the presence of active retrotransposons capable of producing new functional copies at an appropriate rate to compensate for the mutational loss of old ones. Phylogenetic analysis provided strong bootstrap support for a monophyletic origin of plant copia-like retrotransposons, yet showed high diversity within all species. Our results suggest that both vertical transmission of copia-like retrotransposons within G. barbadense lineages, and horizontal transmission between G. barbadense and its progenitors have played major roles in the evolution of copia-like retrotransposons in Gossypium.
Key words: Genome structure, Gossypium, repetitive DNA, polyploidy, sequence diversity, retrotransposons.
Essam A. Zaki*, Abdel Ghany A. Abdel Ghany
Page: 1 - 10