ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-006, July, 2018. © International Scholars Journals
Full Length Research Paper
Isolation and molecular characterization of UTI associated Serratia marcescens TW1 from urine specimens
Tariq A. L.* and John Prabakaran J.
Department of Biotechnology, K.S.R. College of Arts and Science Autonomous, Tiruchengode-637215, Tamilnadu India.
Accepted 15 May, 2018
Abstract
Among the 100 isolates, a bacterial strain TW1 was isolated from the urine tract specimens of infected women admitted at Government hospital, Namakkal District in Tamil Nadu, India, using caprylate thallious agar medium. The organism was characterized by all biochemical tests and showed similarity with Serratia marcescens. The genomic level confirmation done with 16s rDNA primer by submitting the genomic sequence to Gene Bank under ACC.No-GU046545 after comparing, showed 98% sequence similarity with S. marcescens and thus, the strain was named Serrtia marcescens TW1.
Key words: Urinary tract specimens, Serratia marcescens TW1, 16s rDNA genomic analysis.
John Prabakaran J., Tariq A. L.*
Page: 1 - 6
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-004, July, 2018. © International Scholars Journals
Full Length Research Paper
Phytochemical and antimicrobial screening of methanol extract of Heliotropium indicum leaf
Osungunna, M. O.1* and Adedeji, K. A.2
1Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria.
Accepted 24 May, 2018
Abstract
Methanol extract of the leaf of Heliotropium indicum was evaluated for its antimicrobial activity against five bacterial isolates comprising of four Gram-negative namely: Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp. and Proteus mirabilis; and one Gram positive, Staphylococcus aureus at 6.25, 12.5, 25, 50, 100 and 200 mg/ml including phytochemical analysis. While both S. aureus and Klebsiella spp. were inhibited at 50, 100 and 200 mg/ml with minimum inhibitory concentration (MIC) of 3 mg/ml; P. aeruginosa and P. mirabilis with MIC of 10 mg/ml were inhibited at 100 mg/ml and 200 mg/ml. E. coli with MIC of 20 mg/ml was inhibited only at 200 mg/ml concentration of the extract. Phytochemical analysis revealed the presence of plant metabolites as alkaloids, saponin and tannins. While activity-directed assay is advocated, the plant promises to be of tremendous assistance in the treatment of infections with which bacterial isolates used for the study are associated.
Key words: Phytochemical, antimicrobial, Heliotropium indicum alkaloids, saponin, tannin.
M. O.* and Adedeji, K. A., Osungunna
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-004, July, 2018. © International Scholars Journals
Full Length Research Paper
Biochemical role of nitric oxide precursor and antibiotic against typhoid
S. S. Haque
Department of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. E-mail: [email protected].
Accepted 17 June, 2018
Abstract
Typhoid fever remains an underestimated important health problem in many developing countries. It continues to be a global problem with an annual estimate of 1.6 million cases and 600,000 deaths. Salmonella, gram negative bacilli can survive during certain stages of host parasites interaction. There are number of drugs being used for the treatment of typhoid, but increasing occurrence of multidrug resistant (MDR) strain of Salmonella typhi has complicated its management, that thus, it has necessitated the search of formulated drugs for its treatments. Nitric oxide (NO) is a versatile molecule produced in a biological system. Previous studies have suggested that exogenous administration of L-arginine results in increased NO production, indicating that endogenous substrate is insufficient for maximal NO production. Taking these facts in to consideration, it was thought pertinent to see the effect of oral administration of NO precursor that is, L-arginine. Formulation of nitric oxide precursor and antibiotics shows decreases in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 54.24% and 53.91% in 1/2 LArg+1/2 Cip group as compared to bacterial treated groups.
Key words: Salmonella typhi, L-arginine, ciprofloxacin, alanine aminotransferase (ALT), aspartate aminotransferase (AST).
S. S. Haque
Page: 1 - 4
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-007, July, 2018. © International Scholars Journals
Full Length Research Paper
Aerobic and anaerobic bacteria and Candida species in crude milk
Ludmilla Santana de Soares e Barros1*, Silvio Luiz de Oliveira Sóglia1, Marília de Jesus Ferreira2, Margarete de Jesus Rodrigues2 and Marialice Pereira Castelo Branco3
1Professor at the Center of Agrarian, Environmental and Biological Sciences (CCAAB) Federal University of Bahia Recôncavo (UFRB). Rui Barbosa Street, 710. Postal Code 44380-000, Cruz das Almas, Bahia, Brazil.
2Teacher at the Biology Course at CCAAB - UFRB. Rui Barbosa Street, 710. Postal Code 44380-000, Cruz das Almas, Bahia, Brazil.
3Candidate for the Master’s Degree in Animal Science at CCAAB - UFRB. Rui Barbosa Street, 710, Postal Code 44380- 000, Cruz das Almas, Bahia, Brazil.
Accepted 24 May, 2018
Abstract
Since milk is a high nutrition food, it is extremely liable to microbiological contamination. Maximum hygiene in mechanical milking and in health practices management throughout the production process is necessary so that the quality of crude milk is guaranteed. Current research comprises the collection of milk in milk-producing farms during April, May and June 2009 so that the microbiological safety of milk produced in the municipality of Amargosa BA Brazil may be verified. Results show that crude milk samples are unsatisfactory due to the presence of 9.68 and 35.48% of clinical and sub-clinical mastitis, respectively; 12.74% of Staphylococcus aureus, 18.26% of entero-bacteria, 12.5% of Candida albicans, 7.5% of Candida krusei, 10% of Candida tropicalis and 42.5% of other types of Candida. It is mandatory that sanitary education should be provided to milk-producing farmers so that the microbiological quality of milk could be improved.
Key words: Crude milk, microbiology, entero-bacteria, fungi.
Ludmilla Santana de Soares e Barros*, Margarete de Jesus Rodrigues and Marialice Pereira Castelo Branco, Silvio Luiz de Oliveira Sóglia, MarÃlia de Jesus Ferreira
Page: 1 - 7
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-005, July, 2018. © International Scholars Journals
Full Length Research Paper
Community acquired skin infections in children in Abidjan: Methicillin resistant Staphylococcus aureus and exfoliative toxin production
Kacou-N’douba, A.1,2,3*, Kazali, A.1, Koffi, K.S.1,2,3, Ekaza, E.1, Kouablan, A.1, Kangah, T.3, Okpo, S.3, Elogne-Kouamé, C.1 and Dosso, M.1,2
¹Pasteur Institute of Cote d’Ivoire, 01 BP 490 Abidjan 01, Cote d’Ivoire.
²Department of Microbiology, University of Cocody, Unit of Formation and Research of Medical Sciences, Abidjan, Cote d’Ivoire.
3University Hospital Center of Cocody, Abidjan, Cote d’Ivoire.
Accepted 24 June, 2018
Abstract
The worldwide spread of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) skin infections is becoming an emerging problem. These bacteria can produce virulence factors. The objectives of this study were to determine the rate of MRSA and frequency of mecA gene by PCR and detect genes eta and etb encoding exfoliatin A and B. This is a prospective study focused on school children of 5 to 15 years with a cutaneous infection from October 2007 to June 2008. The skin samples taken were allowed to isolate strains of S. aureus by standard bacteriological methods. Susceptibility testing was performed to detect strains of MRSA with cefoxitin disk. Detection of mecA, eta and etb genes was performed using PCR. The rate of MRSA was 14.8%. The mecA gene was present in 2 strains of S. aureus (3.7%). The genes encoding the exfoliatin were detected in 5.6%.
Key words: Community-acquired skin infection, MRSA, exfoliatin A and B.
E , S , Kacou-N’douba , A* , Ekaza , T , K.S , Kouablan , Okpo , Kazali , Koffi , C and Dosso, M , Kangah , Elogne-Kouamé , A , A
Page: 1 - 5
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-005, July, 2018. © International Scholars Journals
Full Length Research Paper
Influence of culture media on mycelial growth and sporulation of some soil dermatophytes compared to their clinical isolates
Mukesh Sharma1* and Meenakshi Sharma2
125, Shri Ram Nagar, Near A.V.M. School, Kalwar Road, Jhotwara, Jaipur-302012, India.
2Microbiology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, India.
Accepted 16 April, 2018
Abstract
Culture media significantly affected the growth, sporulation and conidial discharge of any microorganisms. The present investigation was conducted to examine the effect of broth and agar media on the mycelial growth and fungal sporulation of six species belonging to five genera of fungi. Soil fungal dermatophytes (Trichophyton mentagrophytes, Microsporum gypseum and Microsporum fulvum) were isolated from soil using hair-baiting technique and clinical isolates (Epidermophyton floccosum, Trichophyton schoenleinii and Scopulariopsis sp.) were procured from superficial skin of dermatomycoses patients under the supervision of dermatologists. Variation in mycelial growth and fungal sporulation was observed with media tested. Colony radial growth and sporulation of soil fungi were optimal on Sabouraud’s dextrose medium (SDM) followed by Potato dextrose medium (PDM) and Richard medium (RM). For clinical isolates, the suitable broth media were Potato dextrose medium (PDM) and Yeast extract medium (YEM). Among agar media studied, Sabouraud’s dextrose agar (SDA) and Potato dextrose agar (PDA) media were found most favored for both soil and clinical isolates.
Key words: Clinical isolates, culture media, mycelial growth, soil dermatophytes, sporulation.
Mukesh Sharma*, Meenakshi Sharma
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