ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Short Communication
Rhesus negative pregnant women in a traditional birth home in Abeokuta, Nigeria
Idowu, O.A.1*, Mafiana, C.F. 1 and Sotiloye, Dapo2
1Department of Biological Sciences, University of Agriculture,Abeokuta, Nigeria
2Department of Obstetrics and Gyneacology Federal Medical Centre, Abeokuta, Nigeria.
Accepted 23 July 2003
Abstract
In a survey of 200 pregnant women (mean age 24 years) attending a traditional birth home (TBH) in Abeokuta, Nigeria, 19 (9.5%) were found to be rhesus negative, 8 (42.1%) of which were primigravidae while 11 (57.9%) were multigravidae. 87.5% of the Rhesus negative primigravidae delivered at the TBH without being given the post partum injection of anti-D-gama globulin within 72 h of delivery, thereby having their systems likely sensitized (if baby is rhesus positive) against subsequent pregnancies involving Rhesus positive fetuses. Of the multigravidae involved in this study 27.3% women delivered live babies at the traditional birth home while the remaining 72.7% women were not seen again at the TBH. One woman who has had an induced abortion and was carrying a second pregnancy lost the pregnancy in the course of this study. The knowledge of these women (who were mostly without formal education) on their haematological status is nil. The need to educate the public, especially women patronizing TBH, on the rhesus problem is recommended.
Key words: Pregnancy, Rhesus factor, traditional birth home.
Dapo , Mafiana , O.A* , C.F and Sotiloye, Idowu
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Constructional features of a 15-litre home-made bioreactor for fed-batch fermentations
GUEGUIM KANA, E.B.1*, OLOKE, J.K.1, LATEEF, A. 1 and ZEBAZE KANA, M.G.2
1Biotechnology Centre, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria.
2Advanced Physics Laboratory, Sheda Science and Technology Complex, Abuja, Nigeria.
Accepted 15 July 2003
Abstract
A 15-litre bench-top multipurpose bioreactor was designed and constructed. The vessel is a glass type with a stainless flat headplate incorporating 9 access ports allowing for a variety of interchangeable probes and actuators. The stirring speed ranges between 0 and 250 rpm, the aeration rate (0-2 l/m), the pH control loop uses HI 1131 probe, two 100 ml bottles of HCl and NaOH solutions and operates a close feedback system. The temperature control module is a close loop using a PT 100 RTD thermocouple and an auxiliary vessel containing a cooling solution. The aeration and feed flow rates are open loops. The system incorporates attributes of a good bioreactor design as discussed by Naraendranathan (1998). Sterility is achieved by autoclaving different units of the system. This machine has been tested on an array of local standard fermentation processes.
Key words: Fermentation, bioreactor, control, sensors, actuators.
J.K , GUEGUIM KANA, E.B* , M.G , A and ZEBAZE KANA, OLOKE , LATEEF
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1
S.T. Ogunbanwo*, A.I. Sanni, and A. A. Onilude
Department of Botany and Microbiology University of Ibadan, Nigeria
Accepted 9 July 2003
Abstract
Lactobacillus plantarum F1 and L. brevis OG1 isolated from Nigerian fermented food products, produced bacteriocins that had broad spectrum of inhibition against both pathogenic, food spoilage organisms and various lactic acid bacteria. The test organisms exhibited activities of 6400 and 3200 AU/ml respectively against Escherichia coli NCTC10418 and Enterococcus faecalis EF1, but did not inhibit Candida albicans ATCC10231 and Klebsiella sp. UCH15. Comparison of the antimicrobial spectra and characterization of the two bacteriocins were not identical. Bacteriocin produced by L. brevis OG1 was the most heat stable at 121oC for 60 min, while that of L. plantarum F1 was stable at 121oC for 10 min. The bacteriocins produced by the test isolates maintained full stability after storage for 60 days at – 200 C; partial stability after storage for 120 days at 40C; while activity was not detected after storage for 80 to 120 days at 370C. Bacteriocin produced by L. brevis OG1 was stable at pH range of 2.0 to 8.0 while, that of L. plantarum F1 was found to be stable at pH 2.0 to 6.0. Their active principle was proteinaceous in nature since the bacteriocins were inactivated by proteolytic enzymes, but not by other non–proteolytic enzymes. mitomycin C and uv light did not affect the activity of the bacteriocins, while chloroform extraction completely destroyed their activity. Exposure to surfactant resulted in an increase in the bacteriocin titre, except Nonidet P-40, which led to total loss of bacteriocin activity. The bacteriocins were able to pass through cellulose membranes with 100,000 KDa and 1,000,000 KDa but could not pass through one with a 10,000 KDa and 1,000 KDa molecular weight cut off. The paper concluded that the ability of bacteriocins produced by the test isolates in inhibiting a wide-range of bacteria, is of potential interest for food safety and may have future applications as food preservative.
Key words: Bacteriocins, lactic acid bacteria, indicator organisms, fermented foods, antagonistic activity.
A.I. Sanni, and A. A. Onilude, S.T. Ogunbanwo*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Comparison of multi-locus enzyme and protein gel electrophoresis in the discrimination of five Fusarium species isolated from Egyptian cottons
Ibrahim N. Aly1, Mohmed A. Abdel-Sattar1,
Kamel A. Abd-Elsalam2,3* Mohmed S. Khalil2 and Joseph A. Verreet3
1Suez Canal University, Faculty of Agriculture, Ismailia, Egypt.
2Agricultural Research Center, Plant Pathology Research Institute, Giza, Egypt.
3Christian Albrechts Universität zu Kiel, Institut für Phytopathologie, Kiel, Germany.
Accepetd 21 May 2003
Abstract
Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Fusarium species recovered from cotton-growing areas in Egypt by numerical taxonomy methods. The obtained data revealed that SDS-PAGE and esterase isozymes are more efficient in grouping isolates in their respective species while peroxidase and malate dehydrogenase isozyme has much limited resolution in organizing all isolates in their respective species-specific clusters. A low correlations was detected between geographical origin of isolates and genetic diversity. Results indicate that the estimated inter-specific variation may be more pronounced with protein markers than with isoyzmes when the two approaches are applied to the same populations. The level of genetic variability detected within and between Fusarium spp. accessions with protein and esterase isoyzmes analysis suggests that it is a reliable, efficient, and effective marker technology for determining genetic relationships in Fusarium genus.
Key words: Cotton, Fusarium, Isozymes, polyacrylamide gel electrophoresis.
Ibrahim N. Aly, Kamel A. Abd-Elsalam* Mohmed S. Khalil and Joseph A. Verreet, Mohmed A. Abdel-Sattar
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment
Ndeye Fatou Diaw GUENE, Adama DIOUF and Mamadou GUEYE*
MIRCEN/ Laboratoire commun de microbiologie IRD-ISRA-UCAD, BP 1386, DAKAR, Senegal
Accepted 23 June 2003
Abstract
A field experiment was conducted at Bel Air station, in Dakar using 15N isotope dilution technique and the non nodulating soybean (Glycine max) variety m129 as reference plant to test the compatibility of Dichlorofenthion-thiram (DCT) fungicide to the inoculation of common bean (Phaseolus vulgaris) Paulista variety with both Rhizobium etli ISRA 353 and R. tropici strain ISRA 554. Nodulation was not induced with R. etli ISRA 353 and nitrogen fixation did not occur. With R. tropici ISRA 554, a decrease in nodulation was observed, but nitrogen fixation was not significantly different compared to that of the non DCT-treated common bean.
Key words: Common bean, fungicides, isotope dilution, 15N, nitrogen fixation, nodulation, Phaseolus vulgaris, Rhizobium.
Adama DIOUF and Mamadou GUEYE*, Ndeye Fatou Diaw GUENE
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Full Length Research Paper
Inheritance of resistance to head bug (Eurystylus oldi) in grain sorghum (Sorghum bicolor)
S. E. Aladele1* and I. E. Ezeaku2
1National Centre for Genetic Resources and Biotechnology, P.M.B. 5382, Ibadan, Nigeria
2International Crops Research Institute for the Semi-Arid Tropics, P.M.B. 3491, Kano, Nigeria
Accepted 18 June 2003
Abstract
The inheritance of resistance to head bug (Eurystylus oldi) was studied in ten populations of sorghum derived from crossing three susceptible sorghum elite varieties (ICSV 111, ICSV 112 and ICSV 400), and two resistant sorghum varieties (Malisor 84-7 and KSV 4). Parental lines, F1 and F2 populations were sown on a Randomized Complete Block Design in two replications. Artificial infestation of head bugs on sorghum was employed in carrying out the experiment. Samples of 5 panicles each from every artificially infested plot were observed. Resistance to head bug in sorghum seems to be controlled by a single pair of recessive genes in Malisor 84-7 x ICSV 400 and Malisor 84-7 x ICSV 111. The cross, KSV 4 x ICSV 112 appeared to be controlled by double recessive pair of genes. Head bug population affects quality of grains rather than the yield produced. There is a negative correlation (-0.095) between head bug population and the germination percentage of the grain. Positive relationship exists between glume size and head bug population, which suggests that longer glumes harbour more head bug.
Key Words: Head bug (Eurystylus oldi), Infestation, Anthesis, Resistance, Susceptible, Inheritance.
I. E. Ezeaku, S. E. Aladele*
Page: 1 - 10