ISSN 2736-1756
Research Article
Amar P. Garg, Ramesh C. Uniyal and Archana Kumari, Charu Gupta*
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Research Article
E. van Heerden and L. A. Piater*, A. O. Ojo
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Composition of four essential oils obtained from plants from Cameroon, and their bactericidal and bacteriostatic activity against Listeria monocytogenes, Salmonella enteritidis and Staphylococcus aureus
Fausto Gardini1, Nicoletta Belletti1, Maurice Ndagijimana1, Maria E. Guerzoni1, Francois
Tchoumbougnang2, Paul H. Amvam Zollo3, Claudio Micci4, Rosalba Lanciotti1 and Sylvain L. Sado Kamdem1,5*
1Dipartimento di Scienze degli Alimenti, Università degli Studi di Bologna, Sede di Cesena, Piazza G. Goidanich
2Laboratoire de Biochimie, Faculté des Sciences, Université de Douala, BP 24157 Douala, Cameroun.
3Université de Ngaoundéré, BP 455 Ngaoundéré, Cameroun.
4Cooperativa M. A. R. E., Via Toti 2, 47841 Cattolica, Rimini, Italy.
5Department of Biochemistry, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon.
Accepted 28 January, 2013
Abstract
The composition of four essential oils (EOs) extracted by hydrodistillation from plants of common use in Cameroon (Curcuma longa, Xylopia aethiopica, Zanthoxylum leprieurii L., Zanthoxylum xanthoxyloides) was assessed by gas chromatography-mass. Their bactericidal and bacteriostatic activity was tested in vitro against three food borne pathogenic bacteria: Listeria monocytogenes, Salmonella enteritidis and Staphylococcus aureus. The bacteriostatic activities of this EOs on food borne bacteria were assessed in vitro through optical density measurements. The minimal bactericidal concentrations (MBC) were determined in broth combined with a spot plating method. Z. xanthoxyloides and Z . leprieurii showed a similar composition, with a prevalence of oxygenated monoterpenes (about 58%). The EO of Z. xanthoxyloides was the most effective against the microorganisms tested. Its higher concentration of geraniol could be linked to this higher activity. In almost all cases, the MBC was higher than the maximum concentration tested (3000 ppm). Notwithstanding their low bactericidal effect, the EOs studied showed interesting inhibiting activities against the tested food borne pathogens. S. enteritidis was the most resistant to the bacteriostatic effect of the four EOs. The knowledge of the antimicrobial potential of local plant EOs used in developing countries could help in their choice and their use to improve food safety and shelf-life.
Key words: Essential oils, composition analysis, antimicrobial activity, pathogens.
Rosalba Lanciotti and Sylvain L. Sado Kamdem, Claudio Micci, Fausto Gardini, Maria E. Guerzoni, Maurice Ndagijimana, Paul H. Amvam Zollo, Nicoletta Belletti, Francois Tchoumbougnang
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Enterohaemorrhagic Escherichia coli 0157:H7 Prevalence in meat and vegetables sold in Benin City, Nigeria
Stephen A. Enabulele1* and Nduka Uraih2
1Department of Biological Sciences, College of Natural and applied Sciences, Igbinedion University, Okada, Nigeria.
2Department of Microbiology, Faculty of Life Science, University of Benin, Benin City, Nigeria.
Accepted 23 January, 2013
Abstract
Food samples made up of three meat types of 72 samples each of fresh meat from abattoir and open traditional market and “ready to eat” grilled meat (suya) and three vegetable types consisting of 72 each of cabbage, lettuce and tomatoes all totaling 432 samples, were screened to determine the presence of Escherichia coli 0157:H7 in these food items sold in Benin City, Nigeria. Of the total food samples analyzed E. coli was isolated from 365(84.45%) out of which 10(2.32%) had E. coli 0157:H7. 72(100%) each of the abattoir and open traditional market samples had E. coli, while 41(56.9%) suya samples had E. coli isolated from them. Of these, 5(6.94%) abattoir samples, 2(2.78) open traditional market and 3(4.17%) suya samples had E. coli 0157:H7 present on them. The cabbage, lettuce and tomatoes had 48(66.67%), 68(94.99%) and 64(88.8%) samples with E. coli respectively. E. coli 0157:H7 was not detected in any of the vegetable samples. Most significant of the result is the finding that E. coli 0157:H7 is present in meat sold in Benin City, Nigeria and especially in the “ready to eat” grilled meat ( suya) which is consumed directly without further processing. There is therefore the need for health authorities to put in place measures to prevent possible E. coli 0157:H7 outbreak.
Key words: Escherichia coli 0157:H7, meat, vegetables, suya.
Stephen A. Enabulele*, Nduka Uraih
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Research Article
Fekadu Beyene and Amutha Santhanam, *Esayas Assefa
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Anti-adhesive and anti-invasive activities of an oil based di-herbal extract against methicillin resistant Staphylococcus aureus
Ehsanollah G. R.1*, V. Neela1, Chong P. P.2, Zamberi S. and Mariana N. S.1
1Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health sciences, University Putra Malaysia, Serdang 43400, Selangor, Malaysia.
2Department of Molecular Medicine, Faculty of Medicine and Health sciences, University Putra Malaysia.
Accepted 4 January, 2013
Abstract
Staphylococcus aureus is a major human pathogen capable of causing a wide range of infections. Treatment of S. aureus infections is becoming more challenging with the continuous emergence of new strains of methicillin resistant S. aureus (MRSA) from hospital and community. The aim of the present study is to formulate an oil based combinatorial herbal extract which will not only have antibacterial effect for treatment, but also anti-adhesive and anti-invasive activities against MRSA for to be used as a prophylactic agent. Based on the Indian and Iranian traditional medicine, herbs such as barberry and turmeric which has medicinal properties are tested against MRSA. MRSA and S. aureus strains exposed to oil based di-herbal extract prepared from coconut oil, barberry and turmeric suppressed the partial expression of fibronectin binding protein (FNBP) encoded by fibronectin binding gene (fnbA), that establishes the attachment to and invasion of host cells. Results from the current study showed that the formulated extract has good anti-bacterial, anti-adhesive and anti-invasive activity against S. aureus and MRSA as it showed good zone of inhibition (15 mm) and inhibited the FNB protein synthesis. The formulated combinatorial herbal extract thus could be used for the treatment of S. aureus and MRSA infections as well as for prophylaxis; thus controls the spread of MDR S. aureus strains in hospital and community.
Key words: Anti adhesive, Anti invasive, barberry, coconut oil, turmeric, MRSA.
V. Neela, Chong P. P., Zamberi S. and Mariana N. S., Ehsanollah G. R*
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