ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756, Vol. 17 (1), pp. 001-007, January, 2023. © International Scholars Journals
Full Length Research Paper
Molecular Characterization of CTX-M ESBLs among Pathogenic Enterobacteriaceae isolated from different regions in Sudan
Hisham N Altayb1*, Mohamed A M Siddig2, Nagwa M El Amin3, Ahmed Ibrahim Hashim1 Maowia M. Mukhtar4
1College of medical laboratory science, Sudan University of science and technology, Sudan.
2Botany department, Faculty of Science, University of Khartoum, Sudan.
3Department of microbiology, Faculty of medicine, university of Khartoum, Sudan.
4Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
Accepted 07 November, 2022
Abstract
This was a cross sectional study conducted to detect and characterize CTX-M genes among extended spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolated from different regions in Sudan. A total of 305 of Enterobacteriaceae clinical isolates were collected from different regions in Sudan. ESBLs production was initially screened by cefotaxime, cefepime and Ceftazidime, then confirmed by disk combination method and PCR. DNA sequencing was done to differentiate betwen blaCTX-M genotypes. Escherichia coli was the most predominant isolate (58%), followed by Klebsiella pneumoniae (26.6%), Citrobacter freundii (3.6%), Enterobacter species (6.2%) and Proteus species (5.6%). ESBLs were detected by disk combination method in 128/305 (42%) of the tested isolates; Khartoum State 23/36 (64%), Gizera State 54/100 (54%), Sinnar State 49/92 (53%) and White Nile State 2/77 (2.6%). Three quarters of the ESBLs producers (96/128) were positive for blaCTX-M genes by PCR. The blaCTX-M-15 gene was the most predominant gene 18/23 (78.3%), followed by blaCTX-M-14 3/23(13%), blaCTX-M-27 1/23 (4.3%) and blaCTX-M-98 1/23 (4.3%). There was a transition mutation (substitution of A with G at position 25) in the blaCTX-M gene (ID: KP309815), that affected protein structure. In conclusion blaCTX-M-15 was the most commonly encountered gene and widely spread in different Sudanese regions.
Keywords: blaCTX-M; Beta-Lactamases Genes; Enterobacteriaceae; Sudan