ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756, Vol. 17 (3), pp. 001-004, March, 2023. © International Scholars Journals
Full Length Research Paper
Characterization and Detection of the Scuticociliate Philasterides dicentrarchi in Indo-Pacific Seahorses Hippocampus kuda
Sang Phil Shin1, Jee Eun Han1, Dennis K. Gomez2, Ji Hyung Kim1, Casiano H. Choresca Jr.1, Jin Woo Jun1 and Se Chang Park1*
1Laboratory of Aquatic Animal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151 to 742, Republic of Korea.
2Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines in the Visayas, Miagao, Iloilo 5023, Republic of the Philippines.
Accepted 17 February, 2023
Abstract
The “indo-pacific seahorses”, Hippocampus kuda which had been reared in one of the private commercial aquaria for exhibition were sent to the laboratory for diagnosis. Feces and intestinal contents from 5 seahorses were examined microscopically and revealed numerous scuticociliates. We identified the species of scuticociliate by light (wet mount) and scanning electron microscopy, PCR (polymerase chain reaction) and sequencing. This paper reports the first identification of scuticociliate Philasterides dicentrarchi from seahorse in Korea.
Key words: Indo-pacific seahorse, Philasterides dicentrarchi, scanning electron microscopy, polymerase chain reaction, sequencing.