ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Phtyochemical screening and comparative study of antimicrobial activity of Aloe vera various extracts
Yebpella G. G.1, Adeyemi Hassan M. M.1, Hammuel C.2, Magomya A. M.1, Agbaji A. S.1 and Okonkwo E. M.1
1Industrial Chemical Division, National Research Institute for Chemical Technology, Zaria, Kaduna State, Nigeria.
2Environmental Services and Pollution Control Unit, National Research Institute for Chemical Technology, Zaria, Kaduna State, Nigeria.
Accepted 11 January, 2019
Abstract
The phytochemical screening and comparative study of antimicrobial activity of Aloe vera extracts was carried out. The phytochemical screening revealed the following metabolites: saponins, alkaloids, glycosides, tannins, protein, and flavonoids. While cardiac glyocosides and steroids were absent. The antimicrobial activities of the gel in 10% DMSO, methanol and aqueous extracts of green rind and leaf pulp were carried against some pathogenic clinical isolates, namely, Bacillus subtilis, Staphylococcus aureus, Proteus mirabilis and Candida albicans. The DMSO extract of the gel and the methanol extract of the green rind showed greater potency against tested microorganisms in the order B. Subtilis> S. aureus> C. albicans and S. aureus>C. albicans>B. subtilis>P. mirabilis respectively with different zones of inhibition ranging from 18 to 35 mm gel, 15 to 18 mm for leaf pulp methanol, 20 to 32 mm green rind methanol, also 12 to 14 mm and 14 to 23 mm for leaf pulp aqueous and green rind aqueous respectively. The minimum inhibitory concentration (MIC) of the extracts against organisms ranged from 6.25 to 25 mg/ml while the minimum bactericidal concentration (MBC) was within the range of 12.5 to 50 mg/ml. The study revealed that the green rind methanol extract and gel have greater medicinal potential against B. subtilis.
Key words: Antimicrobial, gel and green rind extract, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and phytochemical screening, Aloe vera.