ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (4), pp. 001-006, April, 2019. © International Scholars Journals
Full Length Research Paper
Genetic characterization of food-and-mouth disease virus WFL strain
Linzhu Ren1, Lin Wang3, Yunzhi Ma4, Xinglong Wang2*
1College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, China.
2Department of Animal Food, The 11th Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun, China.
3Department of Pharmacology, School of Pharmacy, Second military medical university, No.800, Xiangyin Road, Shanghai, China.
4Heping Campus, Jilin University, 5333 Xi’an Road, Changchun, 130062, China.
Accepted 13 February, 2019
Abstract
The complete genome of the foot-and-mouth disease virus (FMDV) strain WFL was cloned and sequenced. The results showed that the complete genome was 8155 nucleotides (nt) in length (including the poly(C) tract, but excluding poly(A) tail) and was composed of a 1059-nt 5'-untranslated region (UTR), a 6969-nt open reading frame, and a 127-nt 3'-UTR. CRE region of 5’UTR was 55nt with 45.5% of G/C, and had a stem-loop. The stem-loops region of 3’UTR can fold into two stem-loops, SL1 and SL2. A phylogenetic tree was constructed based on complete amino acids sequences of WFL strain and reference strains. The strains were divided into 4 clusters. O/ES/2001, HKN/2002, LZ and WFL strain can be divided into one group. It was obvious that WFL strain had a close relationship to LZ strain, which indicated that the WFL strain was of serotype O. There were 16 different deduced amino acid residues between the WFL strain and the LZ strain.
Key words: Food-and-mouth disease virus, sequence, complete genome, untranslated region (UTR).