Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2004

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2004 International Scholars Journals

Full Length Research Paper

A Study on the use of carrot juice in the tissue culture of Daucus carota

D. Puchooa* and R. Ramburn

Faculty of Agriculture, University of Mauritius, Réduit, Mauritius.

Accepted 30 March 2004

Abstract

Effects of using carrot juice in the in vitro growth of Daucus carota were investigated. Callus was the only form of growth obtained after five weeks in culture. The increase in fresh weight, dry weight and moisture content of the explants with increasing concentration of carrot juice in the medium was much lower when compared to either the cytokinin benzyladenine (BA) and/or the auxin picloram. Spectrophotometric and chromatographic analyses revealed the presence of both indole-3-acetic acid (IAA) and abscissic acid (ABA) in the carrot juice, while gibberellic acid was absent.