Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2004

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2004 International Scholars Journals

Short Communication

A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

D. Puchooa

Faculty of Agriculture, University of Mauritius, Réduit, Mauritius. E-mail: [email protected].

Accepted 16 March 2004

Abstract

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.