ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (4), pp. 001-013, April, 2020. © International Scholars Journals
Full Length Research Paper
Comparative analysis on the curing effect of acridine orange (mutagen) on beta - lactamase producing Staphylococus aureus, from bovine and human origin
Onyenwe, N. E.1, Mbata T. I.2* and Adeleke, O. E.1
1Department of Pharmaceutical Microbiology, University of Ibadan, Oyo state, Nigeria.
2Department of Microbiology, Federal Polytechnic Nekede, Owerri, Nigeria.
Accepted 17 October, 2019
Abstract
The effect of the mutagen (acridine orange) on the multi-resistant antibiotics beta lactamase producing Staphylococcus aureus strain of bovine and human origin was investigated and tested individually using iodiometric (cell supervision) method for the level of beta-lactamase produced by each strain and antibiotic – sensitivity screening using single disc agar diffusion method. Analyses revealed that 80% of the isolates from bovine origin produced beta-lactamase while only 20% showed absence of the enzyme, while 68% of the human isolates produced beta-lactamase while only 32% showed absence of the enzyme sensitivity to cefotaxime, cefuroxime and ceftriaxine were observed in both strains of bovine and human origin. At high concentrations of the mutagen (250 µg/ml), zones of inhibitions were low for penicillin, amoxicillin and augmentine at reference MIC’s of 0.25 µg/ml, while the cephalosporins recorded high zones of inhibition at 0.5 and 1.0 µg/ml reference MIC’s in all the strains at 250 and 200 µg/ml of the concentration of the mutagen (acridine orange). The resistance bovine strains producing beta-lactamase resisted curing with acridine orange than the human strains of S. aureus.
Key words: Acridine orange, Staphylococcus aureus, beta-lactamase, iodometric.