ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (9), pp. 001-007, September, 2018. © International Scholars Journals
Full Length Research Paper
Comparison of Norwegian poultry, waterborne and clinical isolates of Campylobacter jejuni by ribotyping
Olav Rosef1*, Algimantas Paulauskas2, Nils Grude3, Camilla Haslekås1 and Andrew Jenkins3
1Telemark University College, Hallvard Eikas Plass 1, 3800 Bø i Telemark, Norway.
2Vytautas Magnus University, Kaunas, Lithuania.
3Unilabs Telelab AS, Skien, Norway.
Accepted 20 July, 2018
Abstract
Campylobacteriosis is the most frequently occurring food and waterborne gastroenteritis in Norway and 90% of cases are caused by Campylobacter jejuni. Campylobacteriosis is epidemiologically associated with use of non-disinfected water and consumption of contaminated poultry. In order to provide molecular epidemiological support for this association isolates from local poultry and water were compared with human isolates from domestic and import infections using ribotyping. Automatic riboprints were performed with the PstI restriction enzyme and RiboPrinter® and compared with a library of recognised patterns (DUP-IDs). Patterns were further compared with each other using GelCompar software. The isolates from human clinical cases showed high heterogeneity. DUP-IDs found among human isolates were also found in 2 isolates from poultry, DUP-PSTI-1146 and DUP-PSTI-2061. Two human isolates were like isolates from water, DUP-PSTI-2073 and DUP-PSTI-1122, but these were from patients infected abroad. This study provides limited support for the importance of poultry as a source of infection and illustrates the need for studies of much larger scope to encompass the huge diversity of strains and sources of C. jejuni.
Key words: Campylobacter, similarity, clustering, genotyping, riboprint.