Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (8), pp. 001-005, August, 2018. © International Scholars Journals

Full Length Research Paper

Evaluation of in-vitro inhibitory effect of honey on some microbial isolate

Raied Taha Al- Naama

Department of Microbiology, College of Dentistry, University of Basrah, Iraq. E-mail: [email protected]. Tel:

009647801298217.

Accepted 24 June, 2018

Abstract

The aim of this study was to investigate the antimicrobial activity of honey sample from Basrah region against certain microbial isolate. Different concentrations (25.0, 50.0, 75.0 and 100.0%) of honey sample where checked for their antimicrobial activities, using some medically important micro-organisms including Escherichia coli, Pseudomonas spp. and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) of the honey sample were determined on the selected micro-organisms by using broth dilution technique. The sample of honey show inhibitory effect in vitro at 50, 75 and 100% concentration on the various investigated micro-organism except at 50% concentration where no inhibition zone on S. aureus. However, no effect was observed at 25% concentration. The MIC for E. coli, Pseudomonas spp. and S. aureus were 6.25, 1.5 and 12.5 mg/ml respectively. The study shows that honey, like antibiotics, has certain organisms sensitive to it, and provides alternative therapy against certain bacteria and is also shown to have antimicrobial action against a broad spectrum of bacteria (both gram- positive and -negative bacteria).

Key words: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, honey, antibiotics, sensitivity, antimicrobial.